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公开(公告)号：US07384746B2
公开(公告)日：2008-06-10
申请号：US10875094
申请日：2004-06-23
申请人： Victor Lyamichev , Zbigniev Skrzypczynski , Hatim T. Allawi , Sarah R. Wayland , Tsetska Y. Takova , Bruce P. Neri , James R. Prudent , Jeff G. Hall
发明人： Victor Lyamichev , Zbigniev Skrzypczynski , Hatim T. Allawi , Sarah R. Wayland , Tsetska Y. Takova , Bruce P. Neri , James R. Prudent , Jeff G. Hall
CPC分类号： C12N9/22 , C07F9/2408 , C07F9/59 , C12Q1/6816 , C12Q1/6823 , C12Q1/683 , C12Q2525/197 , C12Q2565/125 , C12Q2563/107
摘要： The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.
摘要翻译： 本发明涉及新型亚磷酰胺，包括正中和中性化合物。 本发明还提供用于附着到包括固体支持物和核酸的材料的电荷标签，其中电荷标签增加或减少材料的净电荷。 本发明还提供了基于修饰材料和未改性材料之间的电荷差异分离和表征分子的方法。
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公开(公告)号：US07851150B2
公开(公告)日：2010-12-14
申请号：US10740256
申请日：2003-12-18
申请人： James E. Dahlberg , Hatim T. Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn C. Olson-Munoz , LuAnne Chehak , Sarah M. Olson
发明人： James E. Dahlberg , Hatim T. Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn C. Olson-Munoz , LuAnne Chehak , Sarah M. Olson
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2565/1015 , C12Q2561/109 , C12Q2525/207
摘要： The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
摘要翻译： 本发明涉及用于检测和表征干扰RNA如微RNA（miRNA）和小干扰RNA（siRNA）和其他短核酸分子的组合物和方法。 更具体地，本发明涉及用于检测和定量干扰RNA表达的方法。 本发明还提供了miRNA和siRNA的变体和类型的检测。
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公开(公告)号：US20120149018A1
公开(公告)日：2012-06-14
申请号：US13368191
申请日：2012-02-07
申请人： James E. Dahlberg , Hatim T. Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn C. Olson-Munoz , LuAnne Chehak , Sarah M. Olson
发明人： James E. Dahlberg , Hatim T. Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn C. Olson-Munoz , LuAnne Chehak , Sarah M. Olson
CPC分类号： C12Q1/6816 , C12Q2565/1015 , C12Q2561/109 , C12Q2525/207
摘要： The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to improved methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
摘要翻译： 本发明涉及用于检测和表征干扰RNA如微RNA（miRNA）和小干扰RNA（siRNA）和其他短核酸分子的组合物和方法。 更具体地，本发明涉及用于检测和定量干扰RNA表达的改进方法。 本发明还提供了miRNA和siRNA的变体和类型的检测。
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公开(公告)号：US20080187926A1
公开(公告)日：2008-08-07
申请号：US11929878
申请日：2007-10-30
申请人： James E. Dahlberg , Hatim T. Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn C. Olson-Munoz , LuAnne Chehak , Sarah M. Olson
发明人： James E. Dahlberg , Hatim T. Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn C. Olson-Munoz , LuAnne Chehak , Sarah M. Olson
CPC分类号： C12Q1/6816 , C12Q2565/1015 , C12Q2561/109 , C12Q2525/207
摘要： The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to improved methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
摘要翻译： 本发明涉及用于检测和表征干扰RNA如微RNA（miRNA）和小干扰RNA（siRNA）和其他短核酸分子的组合物和方法。 更具体地，本发明涉及用于检测和定量干扰RNA表达的改进方法。 本发明还提供了miRNA和siRNA的变体和类型的检测。
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公开(公告)号：US08609336B2
公开(公告)日：2013-12-17
申请号：US13368191
申请日：2012-02-07
申请人： James E. Dahlberg , Hatim T Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn Olson-Munoz , LuAnne Chehak , Sarah M. Olson
发明人： James E. Dahlberg , Hatim T Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn Olson-Munoz , LuAnne Chehak , Sarah M. Olson
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2565/1015 , C12Q2561/109 , C12Q2525/207
摘要： The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
摘要翻译： 本发明涉及用于检测和表征干扰RNA如微RNA（miRNA）和小干扰RNA（siRNA）和其他短核酸分子的组合物和方法。 更具体地，本发明涉及用于检测和定量干扰RNA表达的方法。 本发明还提供了miRNA和siRNA的变体和类型的检测。
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公开(公告)号：US07482118B2
公开(公告)日：2009-01-27
申请号：US10783557
申请日：2004-02-20
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q2521/301
摘要： The present invention provides novel cleavage agents for the cleavage and modification of nucleic acid. The cleavage agents find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, an endonuclease activity is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新的切割剂。 裂解剂可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用内切核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07582436B2
公开(公告)日：2009-09-01
申请号：US11787843
申请日：2007-04-18
申请人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
发明人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
CPC分类号： C12Q1/6823 , C12Q1/6806 , C12Q1/6853 , C12Q2561/109 , C12Q2525/197
摘要： The present invention provides compositions comprising oligonucleotides that have 3′ end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3′ end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.
摘要翻译： 本发明提供了包含具有3'端基（例如亲脂部分）的寡核苷酸的组合物，其可用于浸润性切割反应，例如INVADER测定。 具体而言，本发明提供含有3'末端基团的寡核苷酸的组合物，其配置用于在具有高信号与背景比的侵入性切割分析中产生可检测信号，以及产生这种组合物的方法。
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公开(公告)号：US20080293930A1
公开(公告)日：2008-11-27
申请号：US11787843
申请日：2007-04-18
申请人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
发明人： Jeff G. Hall , Zbigniev Skrzypczynski , Sarah Wayland , Ned D. Reimer , Luis P. Reynaldo , Joerg Baier , Victor Lyamichev , Bruce P. Neri
IPC分类号： C07H1/00
CPC分类号： C12Q1/6823 , C12Q1/6806 , C12Q1/6853 , C12Q2561/109 , C12Q2525/197
摘要： The present invention provides compositions comprising oligonucleotides that have 3′ end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3′ end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.
摘要翻译： 本发明提供了包含具有3'端基（例如亲脂部分）的寡核苷酸的组合物，其可用于浸润性切割反应，例如INVADER测定。 具体而言，本发明提供含有3'末端基团的寡核苷酸的组合物，其配置用于在具有高信号与背景比的侵入性切割分析中产生可检测信号，以及产生这种组合物的方法。
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公开(公告)号：US06780982B2
公开(公告)日：2004-08-24
申请号：US09777430
申请日：2001-02-06
申请人： Victor Lyamichev , Zbigniew Skrzpczynski , Hatim T. Allawi , Sarah R. Wayland , Tsetska Takova , Bruce P. Neri
发明人： Victor Lyamichev , Zbigniew Skrzpczynski , Hatim T. Allawi , Sarah R. Wayland , Tsetska Takova , Bruce P. Neri
IPC分类号： C07H2100
CPC分类号： C12N9/22 , C07F9/2408 , C07F9/59 , C12Q1/6816 , C12Q1/6823 , C12Q1/683 , C12Q2525/197 , C12Q2565/125 , C12Q2563/107
摘要： The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.
摘要翻译： 本发明涉及新型亚磷酰胺，包括正中和中性化合物。 本发明还提供用于附着到包括固体支持物和核酸的材料的电荷标签，其中电荷标签增加或减少材料的净电荷。 本发明还提供了基于修饰材料和未改性材料之间的电荷差异分离和表征分子的方法。
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公开(公告)号：US07195871B2
公开(公告)日：2007-03-27
申请号：US10290386
申请日：2002-11-07
申请人： Victor Lyamichev , Bruce P. Neri , Jeff Hall , Andrew A. Lukowiak
发明人： Victor Lyamichev , Bruce P. Neri , Jeff Hall , Andrew A. Lukowiak
CPC分类号： C12Q1/6827 , A61K38/00 , C07K2319/02 , C12N9/1252 , C12N9/22 , C12Q2561/109 , C12Q2525/125 , C12Q2525/179 , C12Q2537/143
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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