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33 条记录 (耗时 0.056 秒)

    Methods controlling gene expression
    16.
    发明授权
    Methods controlling gene expression 有权
    控制基因表达的方法

    公开(公告)号：US09085774B2

    公开(公告)日：2015-07-21

    申请号：US11918554

    申请日：2006-04-13

    申请人： Peifeng Ren ,  Hee-Sook Song ,  John McMillan

    发明人： Peifeng Ren ,  Hee-Sook Song ,  John McMillan

    IPC分类号： C12N15/82 ,  C12N15/79

    CPC分类号： C12N15/8218 ,  C12N15/79 ,  C12N15/8216

    摘要： The present invention is in the field of genetics, especially plant genetics, and provides agents capable of controlling gene expression. The present invention specifically provides sequences of naturally occurring, tissue-specifically expressed microRNAs. The invention further provides for transgenic expression constructs comprising sequences encoding said microRNAs. By incorporation of the microRNA encoding sequence the expression from said expression construct is specifically silenced in the tissue where the naturally occurring microRNA is naturally expressed. Thereby the expression profile resulting from the promoter is modulated and leakiness is reduced. The invention further provides for a method for modulating transgenic expression by incorporating sequences encoding said microRNAs into transgenic expression constructs. The compositions and methods of the invention can be used to enhance performance of agricultural relevant crops and for therapy, prophylaxis, research and diagnostics in diseases and disorders, which afflict mammalian species.

    摘要翻译： 本发明涉及遗传学，特别是植物遗传学领域，并提供能够控制基因表达的试剂。 本发明具体提供了天然存在的组织特异性表达的微小RNA的序列。 本发明还提供包含编码所述微小RNA的序列的转基因表达构建体。 通过并入microRNA编码序列，所述表达构建体的表达在天然存在的微RNA天然表达的组织中被特异性沉默。 因此，由启动子产生的表达谱被调节并且泄漏降低。 本发明还提供了通过将编码所述微小RNA的序列并入转基因表达构建体来调节转基因表达的方法。 本发明的组合物和方法可用于增强农业相关作物的性能以及影响哺乳动物物种的疾病和病症的治疗，预防，研究和诊断。

    Method Of Excising A Nucleic Acid Sequence From A Plant Genome
    18.
    发明申请
    Method Of Excising A Nucleic Acid Sequence From A Plant Genome 审中-公开
    从植物基因组中切除核酸序列的方法

    公开(公告)号：US20100162428A1

    公开(公告)日：2010-06-24

    申请号：US12600902

    申请日：2008-05-30

    申请人： Jeffrey A. Brown ,  Fang-Ming Lai ,  Christina E. Roche ,  Hee-Sook Song

    发明人： Jeffrey A. Brown ,  Fang-Ming Lai ,  Christina E. Roche ,  Hee-Sook Song

    IPC分类号： A01H1/00 ,  A01H5/00

    CPC分类号： C12N15/8201 ,  C12N9/22

    摘要： The present invention relates to a method for excising a nucleic acid sequence from the genome of a plant or a plant cell. This method is based on the steps of transforming a plant cell with a construct encoding a DNA double strand break inducing enzyme (DSBI), generating a transgenic plant line, performing a transient assay to analyze the functionality of the transgenic enzyme, crossing the plant line with a line containing a nucleic acid sequence to be excised and performing an immature embryo conversion or a tissue culture regeneration through callus formation. The method can also be reversed, which means that a plant cell is transformed with a construct encoding a nucleic acid sequence to be excised, and the crossing is performed with a plant line containing a DSBI. As an alternative to the crossing step, a re-transformation of a transgenic plant line with a second construct can also be performed. The invention is also directed to a plant obtained by this method, or progeny, propagation material, part, tissue, cell or cell culture, derived from such a plant. Finally, the invention relates to the use of a plant or progeny, propagation material, part, tissue, cell or cell culture, derived from this method, as aliment, fodder or seeds or for the production of pharmaceuticals or chemicals.

    摘要翻译： 本发明涉及从植物或植物细胞的基因组中切除核酸序列的方法。 该方法基于用编码DNA双链断裂诱导酶（DSBI）的构建体转化植物细胞的步骤，产生转基因植物系，进行瞬时测定以分析转基因酶的功能，穿过植物系 含有要切除的核酸序列的线，并通过愈伤组织形成进行未成熟胚转化或组织培养再生。 该方法也可以相反，这意味着用编码待切除的核酸序列的构建体转化植物细胞，并且用含有DSBI的植物系进行杂交。 作为交叉步骤的替代方案，也可以进行具有第二构建体的转基因植物系的再转化。 本发明还涉及通过该方法获得的植物，或从这种植物衍生的后代，繁殖材料，部分，组织，细胞或细胞培养物。 最后，本发明涉及来源于该方法的植物或后代，繁殖材料，部分，组织，细胞或细胞培养物作为营养物，饲料或种子或用于生产药物或化学品的用途。

    Nucleotide sequences encoding anthranilate synthase
    19.
    发明授权
    Nucleotide sequences encoding anthranilate synthase 失效
    编码邻氨基苯甲酸合酶的核苷酸序列

    公开(公告)号：US06563025B1

    公开(公告)日：2003-05-13

    申请号：US09264854

    申请日：1999-03-08

    申请人： Hee-Sook Song ,  Jeffrey E. Brotherton ,  Jack M. Widholm

    发明人： Hee-Sook Song ,  Jeffrey E. Brotherton ,  Jack M. Widholm

    IPC分类号： C12N1509

    CPC分类号： C12N15/8216 ,  C12N9/88 ,  C12N15/8209 ,  C12N15/8222

    摘要： A 5-methyltryptophan resistant Nicotiana anthranilate synthase genes are disclosed, in particular the ASA2 gene of Nicotiana tabacum. Constructs containing the genes are also disclosed. This genes are useful for transforming plant cells and producing transformed plants and progenies which, compared to untransformed plant cells and plants, are more resistant to tryptophan analogs and/or produce increased levels of tryptophan. The transformed seeds are also disclosed. The genes are also useful as selectable markers. The amino acid sequence of ASA2, purified ASA2, and recombinant ASA2 are also disclosed.

    摘要翻译： 公开了一种5-甲基色氨酸耐药烟碱氨基酸合酶基因，特别是烟草属的ASA2基因。 还披露了含有这些基因的构建体。 该基因可用于转化植物细胞并产生转化的植物和后代，其与未转化的植物细胞和植物相比对色氨酸类似物更有抗性和/或产生增加的色氨酸水平。 转化的种子也被披露。 这些基因也可用作选择标记。 还公开了ASA2的氨基酸序列，纯化的ASA2和重组ASA2。