公开(公告)号：US20230204540A1

公开(公告)日：2023-06-29

申请号：US18068763

申请日：2022-12-20

Applicant: IMEC VZW ,  Katholieke Universiteit Leuven, KU LEUVEN R&D

Inventor： Willem Van Roy ,  Chengxun Liu ,  Tinne De Moor

IPC: G01N27/447 ,  B01L3/00

CPC classification number: G01N27/44791 ,  B01L3/502761 ,  G01N27/44713 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0838 ,  B01L2400/0406 ,  B01L2400/0421

Abstract: An electric field gradient focusing device is provided that includes: (i) a fluidic channel having an inlet and an outlet for a fluid, (ii) a first actuator configured to induce a fluid flow in the fluidic channel from the inlet to the outlet via AC electroosmosis, and (iii) a second actuator configured to generate a DC electric field gradient along at least part of the fluidic channel.

公开(公告)号：US20230191411A1

公开(公告)日：2023-06-22

申请号：US18068779

申请日：2022-12-20

Applicant: IMEC VZW ,  Katholieke Universiteit Leuven, KU LEUVEN R&D

Inventor： Chengxun Liu ,  Camila Dalben Madeira Campos ,  Xavier Rottenberg

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2400/0424 ,  B01L2300/0864

Abstract: Embodiments for sorting particles are provided that include a microfluidic channel configured to receive a microfluidic flow that comprises a plurality of particles having different characteristics, the microfluidic channel having a plurality of output flow channels, a first detector configured to detect the location of the particles, a plurality of actuators located along the direction of the microfluidic flow and defining a sorting electrode arrangement. The microfluidic device further comprises a controller configured to receive signals from the first detector and to provide force field profiles for each of the plurality of particles, wherein each force field profile comprises a plurality of deflection force settings along the direction of the microfluidic flow. The controller individually addresses the plurality of actuators to generate a plurality of actuation inducing fields along the direction of the microfluidic flow to generate the deflection force settings in the force field profiles.

公开(公告)号：US11454583B2

公开(公告)日：2022-09-27

申请号：US17133771

申请日：2020-12-24

Applicant: IMEC VZW ,  Katholieke Universiteit Leuven, KU LEUVEN R & D

Inventor： Chengxun Liu ,  Andim Stassen ,  Ying Ting Set

IPC: G01N15/10 ,  G01N15/00

Abstract: An example includes a field-flow fractionation device for the continuous separation of sample components including a channel comprising a sample inlet and a plurality of sample outlets, the channel being for coupling to a flow generator for translocating the sample components along the channel in a first direction from the sample inlet to the plurality of sample outlets, an actuator, which is not the flow generator, coupled to the channel, for translocating the sample components in a second direction, at a first angle with the first direction, an array of electrodes for connection to an AC power source, being in a path taken by the sample components in the channel, arranged in a plurality of rows, and in such a way that adjacent rows can be set at different potentials and every other row can be set at the same potential.

公开(公告)号：US20210199556A1

公开(公告)日：2021-07-01

申请号：US17133771

申请日：2020-12-24

Applicant: IMEC VZW ,  Katholieke Universiteit Leuven, KU LEUVEN R & D

Inventor： Chengxun Liu ,  Andim Stassen ,  Ying Ting Set

IPC: G01N15/10

Abstract: An example includes a field-flow fractionation device for the continuous separation of sample components including a channel comprising a sample inlet and a plurality of sample outlets, the channel being for coupling to a flow generator for translocating the sample components along the channel in a first direction from the sample inlet to the plurality of sample outlets, an actuator, which is not the flow generator, coupled to the channel, for translocating the sample components in a second direction, at a first angle with the first direction, an array of electrodes for connection to an AC power source, being in a path taken by the sample components in the channel, arranged in a plurality of rows, and in such a way that adjacent rows can be set at different potentials and every other row can be set at the same potential.

公开(公告)号：US20190351408A1

公开(公告)日：2019-11-21

申请号：US16472841

申请日：2017-12-20

Applicant: IMEC VZW

Inventor： Chengxun Liu ,  Rodrigo Sergio Wiederkehr

IPC: B01L3/00

Abstract: A micro-fluidic device is provided to sort out objects from a liquid stream. The device comprises a first channel comprising a first liquid and a second channel comprising a second liquid and the first liquid, and a third channel. The second channel is connected to the first channel and the channels are positioned such that a jet flow coming from the second channel can deflect objects in the first liquid into the third channel. The first liquid is a liquid which has a higher viscosity than water and the second liquid may be the same as or different from the first liquid. The micro-fluidic device is adapted for generating the jet flow in the second liquid.

公开(公告)号：US20250066817A1

公开(公告)日：2025-02-27

申请号：US18813902

申请日：2024-08-23

Applicant: IMEC VZW

Inventor： Koen Johannes Hubertus Gerardus de Wijs ,  Maarten Fauvart ,  Chengxun Liu ,  Tim Stakenborg

IPC: C12N15/87 ,  C12M1/42

Abstract: A method and system for electroporation of a biological cell in a fluidic channel is provided. The method includes: introducing a fluidic flow in a first direction in the fluidic channel wherein the fluidic flow comprises the cell; applying a first electrical field to the cell in the fluidic flow to produce a first electroporation at a first position in the fluidic channel; measuring an impedance of the cell in the fluidic flow at a second position in the fluidic channel; determining a second electrical field depending on the impedance measurement of the cell in the fluidic flow at the second position in the fluidic channel; and applying the second electrical field to the cell in the fluidic flow to produce a second electroporation at a third position in the fluidic channel, wherein the first, second and third positions are sequentially arranged in the first direction of the fluidic flow.

公开(公告)号：US12181448B2

公开(公告)日：2024-12-31

申请号：US17129211

申请日：2020-12-21

Applicant: IMEC VZW ,  Katholieke Universiteit Leuven, KU LEUVEN R & D

Inventor： Chengxun Liu ,  Andim Stassen ,  Ying Ting Set

IPC: G01N30/00 ,  B01L3/00 ,  B03C5/02

Abstract: A method for continuously separating components from a sample includes providing a field-flow fractionation device including: a channel coupled to a flow generator for translocating the sample components along the channel in a first direction, an actuator for translocating the sample components in a second direction, at an angle with the first direction, and an array of electrodes electrically or capacitively connected to an AC power source, operating the actuator so as to translocate the sample components in a second direction at an angle with the first direction, operating the AC power source so as to generate an AC electric field between adjacent rows, and operating the flow generator, collecting sample components from the sample outlets.

公开(公告)号：US11772095B2

公开(公告)日：2023-10-03

申请号：US16771025

申请日：2018-12-20

Applicant: IMEC VZW

Inventor： Chengxun Liu ,  Bivragh Majeed ,  Abdulkadir Yurt

IPC: B01L3/00 ,  G01N15/14 ,  G01N15/00

CPC classification number: B01L3/502761 ,  B01L3/502707 ,  B01L3/502715 ,  G01N15/1434 ,  B01L2200/0652 ,  B01L2200/12 ,  B01L2300/0654 ,  B01L2300/0887 ,  G01N15/1484 ,  G01N2015/0065 ,  G01N2015/149

Abstract: A microfluidic routing device for routing objects of interest in a microfluidic flow includes a substrate; a first layer provided on the substrate, in which the first layer forms a bottom wall of a microfluidic channel. At least two holes through the first layer form respectively an inlet and an outlet for the microfluidic channel The microfluidic routing device further includes a second layer spaced away from the first layer, in which the second layer forms a top wall of the microfluidic channel. The second layer is configured to transmit an optical signal from the microfluidic channel. The microfluidic routing device includes an actuator for actuating the objects of interest in a sorting junction of the microfluidic channel.

公开(公告)号：US11358144B2

公开(公告)日：2022-06-14

申请号：US16472841

申请日：2017-12-20

Applicant: IMEC VZW

Inventor： Chengxun Liu ,  Rodrigo Sergio Wiederkehr

IPC: B01L3/00

Abstract: A micro-fluidic device is provided to sort out objects from a liquid stream. The device comprises a first channel comprising a first liquid and a second channel comprising a second liquid and the first liquid, and a third channel. The second channel is connected to the first channel and the channels are positioned such that a jet flow coming from the second channel can deflect objects in the first liquid into the third channel. The first liquid is a liquid which has a higher viscosity than water and the second liquid may be the same as or different from the first liquid. The micro-fluidic device is adapted for generating the jet flow in the second liquid.

公开(公告)号：US11313489B2

公开(公告)日：2022-04-26

申请号：US17135192

申请日：2020-12-28

Applicant: IMEC VZW

Inventor： Lei Zhang ,  Tim Stakenborg ,  Chengxun Liu ,  David Cheyns

IPC: F16K31/128 ,  F16K7/10 ,  F16K7/12 ,  F16K31/00 ,  F16K11/24

Abstract: Example embodiments relate to microfluidic devices for controlling pneumatic microvalves. One embodiment includes a microfluidic device for independently controlling a plurality of pneumatic microvalves. The microfluidic device is couplable to a pressure source. The microfluidic device includes a first substrate. The microfluidic device also includes a flexible membrane covering the first substrate. Additionally, the microfluidic device includes a second substrate covering the flexible membrane. Further, the microfluidic device includes one or more fluidic channels at least partially defined in the first substrate. In addition, the microfluidic device includes a pressure couplable to the pressure source and branching into a plurality of pressure channels. Still further, the microfluidic device includes at least one pressure control switch per pressure channel.