公开(公告)号：US20210214671A1

公开(公告)日：2021-07-15

申请号：US16740420

申请日：2020-01-11

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Erik Zimmerman ,  Emily Feldman ,  Benjamin Mijts ,  Andrew Garst

IPC: C12M1/36 ,  C12N15/10

Abstract: The present disclosure provides compositions, automated multi-module instruments and methods to increase the percentage of edited mammalian cells in a cell population when employing nucleic-acid guided editing.

公开(公告)号：US10801008B1

公开(公告)日：2020-10-13

申请号：US16930269

申请日：2020-07-15

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/26 ,  C12N15/87 ,  B01D71/02 ,  B01D63/08 ,  B01D69/06

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US10723995B1

公开(公告)日：2020-07-28

申请号：US16820292

申请日：2020-03-16

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/12 ,  B01D25/00 ,  B01D63/08 ,  C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00 ,  B01D69/06 ,  B01D71/02

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200216795A1

公开(公告)日：2020-07-09

申请号：US16823269

申请日：2020-03-18

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US10704033B1

公开(公告)日：2020-07-07

申请号：US16714320

申请日：2019-12-13

Applicant: Inscripta, Inc.

Inventor： Juhan Kim ,  Benjamin Mijts ,  Aamir Mir ,  Andrew Garst ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/90 ,  C12N15/10 ,  C12N15/81 ,  C12N15/85 ,  C12N15/74

Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.

公开(公告)号：US10689669B1

公开(公告)日：2020-06-23

申请号：US16740418

申请日：2020-01-11

Applicant: Inscripta, Inc.

Inventor： Emily Feldman ,  Benjamin Mijts ,  Aamir Mir ,  Erik Zimmerman ,  Andrew Garst

IPC: C12M3/00 ,  C12M1/00 ,  C12N15/87 ,  C12M1/34

Abstract: The present disclosure provides automated multi-module instruments, compositions and methods to increase the percentage of edited mammalian cells in a cell population when employing nucleic-acid guided editing.

公开(公告)号：US10655114B1

公开(公告)日：2020-05-19

申请号：US16798309

申请日：2020-02-22

Applicant: Inscripta, Inc.

Inventor： Benjamin Mijts ,  Juhan Kim ,  Aamir Mir ,  Kyle Seamon ,  Andrew Garst

IPC: C12N9/22

Abstract: The present disclosure provides engineered RNA-guided enzymes for editing live cells.

公开(公告)号：US12180502B2

公开(公告)日：2024-12-31

申请号：US17829316

申请日：2022-05-31

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C07K14/195 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20220389417A1

公开(公告)日：2022-12-08

申请号：US17829316

申请日：2022-05-31

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/11 ,  C12N15/90 ,  C12N9/22

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US11512297B2

公开(公告)日：2022-11-29

申请号：US17522399

申请日：2021-11-09

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Tian Tian ,  Daniel Held

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or “RGN”)/single-strand binding protein (“SSB”) fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (“SSAP”) interactions to drive enhanced recruitment.