公开(公告)号：US11203762B2

公开(公告)日：2021-12-21

申请号：US16952024

申请日：2020-11-18

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

公开(公告)号：US11053507B2

公开(公告)日：2021-07-06

申请号：US17072023

申请日：2020-10-15

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Charles Johnson ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US20210147858A1

公开(公告)日：2021-05-20

申请号：US16952024

申请日：2020-11-18

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N9/22 ,  C12N1/20

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

公开(公告)号：US20200056192A1

公开(公告)日：2020-02-20

申请号：US16540767

申请日：2019-08-14

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Charles Johnson ,  Clint Davis

IPC: C12N15/70 ,  C12M3/00 ,  C12M1/42 ,  C12M1/12 ,  C12N15/90

Abstract: The present disclosure provides methods, automated modules, and instruments for enrichment of live cells that have been edited by nucleic acid-guided nuclease genome editing. The disclosure provides improved methods and modules—including high throughput methods and modules—for enriching for cells that have been subjected to editing.

公开(公告)号：US20190292568A1

公开(公告)日：2019-09-26

申请号：US16358461

申请日：2019-03-19

Applicant: Inscripta, Inc.

Inventor： Christopher Abraham ,  Charles Johnson ,  Clint Davis ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/90 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure thus provides methods for higher efficiency cell editing that maintain the viability of the cells. In certain aspects, the present disclosure provides systems and instruments for automated methods of multiplexed nuclease-directed genome editing using nuclease expression under a constitutive promoter. In specific aspects, the constitutive promoter is an insulated promoter. In other aspects, the constitutive promoter is a heterologous promoter. In still other aspects, the constitutive promoter is a synthetic promoter.

公开(公告)号：US11965154B2

公开(公告)日：2024-04-23

申请号：US16545097

申请日：2019-08-20

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Isaac Wagner ,  Clint Davis ,  Julia Swavola ,  Phillip Belgrader

IPC: C12N1/20 ,  B01L3/00 ,  C12M1/00 ,  C12M1/42 ,  C12M3/00 ,  C12M3/04 ,  C12N9/22 ,  C12N13/00 ,  G01N35/00 ,  G01N35/10

CPC classification number: C12N1/20 ,  B01L3/502 ,  C12M27/10 ,  C12M29/04 ,  C12M35/00 ,  C12M35/02 ,  C12M45/00 ,  C12N9/22 ,  C12N13/00 ,  G01N35/00722 ,  G01N35/10 ,  B01L2200/16 ,  B01L2300/087 ,  C12M23/44 ,  G01N2035/009

Abstract: The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

公开(公告)号：US11634719B2

公开(公告)日：2023-04-25

申请号：US17676210

申请日：2022-02-20

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Tian Tian ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US11319542B2

公开(公告)日：2022-05-03

申请号：US17536067

申请日：2021-11-28

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.

公开(公告)号：US20220081693A1

公开(公告)日：2022-03-17

申请号：US17536067

申请日：2021-11-28

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome

公开(公告)号：US20210371799A1

公开(公告)日：2021-12-02

申请号：US17401362

申请日：2021-08-13

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Michael Graige ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M3/00 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.