公开(公告)号：US5891648A

公开(公告)日：1999-04-06

申请号：US748415

申请日：1996-11-13

申请人： Mark T. Martin ,  Rodger G. Smith ,  Michael J. Darsley ,  David M. Simpson ,  Gary F. Blackburn

发明人： Mark T. Martin ,  Rodger G. Smith ,  Michael J. Darsley ,  David M. Simpson ,  Gary F. Blackburn

IPC分类号： G01N33/53 ,  B01J20/281 ,  C12N5/10 ,  C12N7/02 ,  C12N15/10 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/70 ,  C40B40/02 ,  G01N30/88 ,  G01N33/569 ,  C12Q1/25

CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12Q1/02 ,  C12Q1/6811 ,  C12Q1/70 ,  G01N33/56983

摘要： Disclosed and claimed are methods for selecting a recombinant virus, phage or cell expressing a catalytic antibody or catalytic portion thereof, or for selecting catalytic activity by a moiety. The method employs reaction-based selection for catalytic activity. The method can also be used to concentrate (increase the proportion of catalytic to non-catalytic moieties) a sample containing a catalytic moiety or viruses, phages or cells expressing a catalytic moiety. The selection or concentrating can be by employing a mechanism-based inhibitor, catalysis-accelerated movement, surface binding, changes in enthalpic component of binding as a function of temperature, or changes in binding by competition, or combinations thereof. The invention also comprehends a method for producing a recombinant virus or a cell-line expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof; and, this method can include infecting a suitable host with viruses which are screened for the expression. In addition, recombinant viruses and cell-lines so expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof are also disclosed and claimed.

公开(公告)号：US5593969A

公开(公告)日：1997-01-14

申请号：US123590

申请日：1993-09-17

申请人： Balreddy Kamireddy ,  Michael J. Darsley ,  David M. Simpson ,  Richard J. Massey

发明人： Balreddy Kamireddy ,  Michael J. Darsley ,  David M. Simpson ,  Richard J. Massey

IPC分类号： A61K38/00 ,  C07H13/06 ,  C07H15/04 ,  C07K16/12 ,  A61K31/70 ,  C07H3/02 ,  C07H3/04

CPC分类号： C07H13/06 ,  C07H15/04 ,  C07K16/1203 ,  A61K38/00

摘要： A compound of the formula: ##STR1## wherein: each of R.sub.1, R.sub.1 ', R.sub.2 and R.sub.2 ' independent of each other is a substituted or unsubstituted, branched or linear C.sub.1-12 alkyl, alkene or alkyne group, R.sub.3 is OH, OCH.sub.3, CH.sub.2 COOH or ##STR2## wherein each of R.sub.2" and R.sub.2 '41 independent of each other is a substituted or unsubstituted, branched or linear C.sub.1-12 alkyl, alkene or alkyne group and:A=NH.sub.2, X=P(OH), Y=Z=C, B=OCH.sub.3, orA=OH, X=P(OH), X=Z=C, B (if present)=OCH.sub.3, orA=OCO(CH.sub.2).sub.n NH.sub.2, X=P(OH), Y=Z=C, B=OCH.sub.3,wherein n=1-10, orA=OH, X=P(OH), Y=Z=C, B=O(CH.sub.2).sub.n CO.sub.2 H, wherein n=1-10, orA=OH, X=P(OH), Y=Z=C, B=(CH.sub.2).sub.n CO.sub.2 H, wherein n=1-10, orA=NH.sub.2, X=Z=C, Y=P(OH), B=OCH.sub.3, orA=OH, X=Z=C, Y=P(OH), B (if present)=OCH.sub.3, orA=OCO(CH.sub.2).sub.n NH.sub.2, X=Z=C, Y=P(OH), B=OCH.sub.3, wherein n=1-10, orA=OH, X=Z=C, Y=P(OH), B=O(CH.sub.2).sub.n CO.sub.2 H, wherein n=1-10, orA=OH, X=Z=C, Y=P(OH), B=(CH.sub.2).sub.n CO.sub.2 H, wherein n=1-11, orA=NH.sub.2, X=Y=C, Z=P(OH), B=OCH.sub.3, orA=OH, X=Y=C, Z=P(OH), B (if present)=OCH.sub.3, orA=OCO(CH.sub.2).sub.n NH.sub.2, X=Y=C, Z=P(OH), B=OCH.sub.3, wherein n=1-10, orA=OH, X=Y=C, Z=P(OH), B=O(CH.sub.2).sub.n CO.sub.2 H, wherein n=1-10, orA=OH, X=Y=C, Z=P (OH), B=(CH.sub.2).sub.n CO.sub.2 H and n=1-11 is disclosed. The compounds may be use to inhibit binding of Lipid A to Lipid A receptors.

摘要翻译： 下式的化合物：其中：R 1，R 1'，R 2和R 2'彼此独立地为取代或未取代的支链或直链C 1-12烷基，烯烃或炔基，R 3为 OH，OCH 3，CH 2 COOH或者其中每个R 2“和R 21'彼此独立地是取代或未取代的支链或直链C 1-12烷基，烯烃或炔基，并且：A = NH 2，X = P （OH），Y = Z = C，B = OCH 3或A = OH，X = P（OH），X = Z = C，B（如果存在）= OCH 3或A = OCO（CH 2）n NH 2，X = P（OH），Y = Z = C，B = OCH 3，其中n = 1-10，或A = OH，X = P（OH），Y = Z = C，B = O（CH 2）n CO 2 H，其中 n = 1-10，或A = OH，X = P（OH），Y = Z = C，B =（CH2）nCO2H，其中n = 1-10，或A = NH2，X = Z = C，Y = P（OH），B = OCH3或A = OH，X = Z = C，Y = P（OH），B（如果存在）= OCH 3或A = OCO（CH 2）n NH 2，X = Z = C ，Y = P（OH），B = OCH 3，其中n = 1-10，或A = OH，X = Z = C，Y = P（OH），B = O（CH 2）n CO 2 H，其中n = 10或A = OH，X = Z = C，Y = P（OH），B =（CH2）nCO2H，其中n = 1-11或A = NH2，X = Y = C，Z = P ），B = OCH 3或A = OH，X = Y = C，Z = P（OH），B（如果存在）= OCH 3或A = OCO（CH 2）n NH 2，X = Y = C，Z = P （OH），B = OCH 3， 其中n = 1-10，或A = OH，X = Y = C，Z = P（OH），B = O（CH 2）n CO 2 H，其中n = 1-10，或A = OH，X = Y = C ，Z = P（OH），B =（CH2）nCO2H和n = 1-11。 该化合物可用于抑制脂质A与脂质A受体的结合。