公开(公告)号：US20090111188A1

公开(公告)日：2009-04-30

申请号：US12286668

申请日：2008-10-01

申请人： Qihua C. Cai ,  Vipula K. Shukla ,  Joseph F. Petolino ,  Lisa W. Baker ,  Robbi J. Garrison ,  Ryan C. Blue ,  Jon C. Mitchell ,  Nicole L. Arnold ,  Sarah E. Worden ,  Jeffrey Miller ,  Fyodor Urnov

发明人： Qihua C. Cai ,  Vipula K. Shukla ,  Joseph F. Petolino ,  Lisa W. Baker ,  Robbi J. Garrison ,  Ryan C. Blue ,  Jon C. Mitchell ,  Nicole L. Arnold ,  Sarah E. Worden ,  Jeffrey Miller ,  Fyodor Urnov

IPC分类号： C12N15/10

CPC分类号： C07K14/4702 ,  C07K2319/81 ,  C12N15/8213 ,  C12N15/8243

摘要： Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.

摘要翻译： 本文公开了包含CCHC锌配位残基的锌指。 还描述了锌指蛋白和包含这些CCHC锌指的融合蛋白以及编码这些蛋白的多核苷酸。 还描述了使用这些蛋白质进行基因编辑和基因调控的方法。

公开(公告)号：US09695432B2

公开(公告)日：2017-07-04

申请号：US13011666

申请日：2011-01-21

申请人： Sean M. Russell ,  Joseph F. Petolino

发明人： Sean M. Russell ,  Joseph F. Petolino

IPC分类号： C12N15/82 ,  C12N9/22 ,  A01H1/02

CPC分类号： C12N15/8213 ,  A01H1/02 ,  C12N9/22 ,  C12N15/8241 ,  C12N15/8265

摘要： A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.

公开(公告)号：US08592645B2

公开(公告)日：2013-11-26

申请号：US12925491

申请日：2010-10-22

申请人： Russell DeKelver ,  Manju Gupta ,  Jeffrey C. Miller ,  Stephen Novak ,  Joseph F. Petolino

发明人： Russell DeKelver ,  Manju Gupta ,  Jeffrey C. Miller ,  Stephen Novak ,  Joseph F. Petolino

IPC分类号： A01H5/00 ,  C07K14/00 ,  C12N5/04 ,  C12N15/82

CPC分类号： C12N15/8218 ,  C07K14/415 ,  C12N9/22 ,  C12N15/8216 ,  C12N15/8247 ,  C12Y203/01041 ,  C12Y301/00

摘要： The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.

摘要翻译： 本公开涉及靶向参与脂肪酸生物合成的植物中的基因的工程化锌指蛋白。 还提供了使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。

公开(公告)号：US20110167521A1

公开(公告)日：2011-07-07

申请号：US12925491

申请日：2010-10-22

申请人： Russell DeKelver ,  Manju Gupta ,  Jeffrey C. Miller ,  Stephen Novak ,  Joseph F. Petolino

发明人： Russell DeKelver ,  Manju Gupta ,  Jeffrey C. Miller ,  Stephen Novak ,  Joseph F. Petolino

IPC分类号： A01H5/10 ,  C07K2/00 ,  C07K19/00 ,  C07H21/00 ,  C12N15/82 ,  C12N5/10 ,  A01H5/00

CPC分类号： C12N15/8218 ,  C07K14/415 ,  C12N9/22 ,  C12N15/8216 ,  C12N15/8247 ,  C12Y203/01041 ,  C12Y301/00

摘要： The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.

摘要翻译： 本公开涉及靶向参与脂肪酸生物合成的植物中的基因的工程化锌指蛋白。 还提供了使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。

公开(公告)号：US20110008833A1

公开(公告)日：2011-01-13

申请号：US12509766

申请日：2009-09-09

申请人： Joseph F. Petolino ,  Qihua C. Cai ,  Weiting W. Ni

发明人： Joseph F. Petolino ,  Qihua C. Cai ,  Weiting W. Ni

IPC分类号： C12P21/00

CPC分类号： C12N15/8201

摘要： The present invention relates to methods and compositions for the expression of protein. Embodiments of methods may comprise the cleavage and repair of a nucleotide sequence encoding a highly expressed protein leading to a reduction in the expression of the highly expressed protein.

摘要翻译： 本发明涉及蛋白质表达的方法和组合物。 方法的实施方案可以包括编码高表达蛋白质的核苷酸序列的切割和修复，导致高度表达的蛋白质的表达降低。

公开(公告)号：US07166768B2

公开(公告)日：2007-01-23

申请号：US10391707

申请日：2003-03-19

申请人： Bridget D. Kosegi ,  Jeffrey R. Beringer ,  Asha Mehra Palta ,  Joseph F. Petolino ,  Raghav Ram

发明人： Bridget D. Kosegi ,  Jeffrey R. Beringer ,  Asha Mehra Palta ,  Joseph F. Petolino ,  Raghav Ram

IPC分类号： C12N15/87

CPC分类号： C12N15/8207

摘要： Embryogenic cotton suspension cultures can be transformed by elongated, needle-like structures called “whiskers”. The process comprises the agitation of cotton suspension cultures in the presence of DNA and whiskers, whereby DNA uptake and integration thereof is facilitated.

摘要翻译： 胚胎生长棉悬浮培养物可以被称为“晶须”的细长的针状结构转化。 该方法包括在DNA和晶须的存在下搅拌棉花悬浮培养物，从而促进DNA吸收和整合。

公开(公告)号：US06730824B2

公开(公告)日：2004-05-04

申请号：US10024070

申请日：2001-12-18

申请人： Joseph F. Petolino ,  Dayakar R. Pareddy ,  Nicole L. Hopkins ,  Katherine Armstrong

发明人： Joseph F. Petolino ,  Dayakar R. Pareddy ,  Nicole L. Hopkins ,  Katherine Armstrong

IPC分类号： C12N1582

CPC分类号： C12N15/8216 ,  C12N15/8207 ,  C12N15/8222

摘要： Cotton embryogenic callus tissues can be transformed by elongated, needle-like structures called “whiskers”. The process comprises the agitation of plant cell aggregates and plant tissues of the plant to be transformed in the presence of DNA and whiskers, whereby DNA uptake and integration thereof is facilitated.

摘要翻译： 棉花胚发生愈伤组织可以通过称为“晶须”的细长的针状结构转化。 该方法包括在DNA和晶须的存在下搅拌待转化的植物的植物细胞聚集体和植物组织，由此促进其DNA吸收和整合。

公开(公告)号：US06699984B1

公开(公告)日：2004-03-02

申请号：US09643971

申请日：2000-08-22

申请人： Michael Ainley ,  Katherine Armstrong ,  Scott Belmar ,  Otto Folkerts ,  Nicole Hopkins ,  Michael A. Menke ,  Dayakar Pareddy ,  Joseph F. Petolino ,  Kelley Smith ,  Aaron Woosley

发明人： Michael Ainley ,  Katherine Armstrong ,  Scott Belmar ,  Otto Folkerts ,  Nicole Hopkins ,  Michael A. Menke ,  Dayakar Pareddy ,  Joseph F. Petolino ,  Kelley Smith ,  Aaron Woosley

IPC分类号： C07H2104

CPC分类号： C12N9/0065 ,  C12N15/8216 ,  C12N15/8222 ,  C12N15/8227

摘要： Regulatory sequences derived from the maize per5 gene have utility in plant biotechnology.

摘要翻译： 源自玉米per5基因的调节序列在植物生物技术中具有实用价值。

公开(公告)号：US09249422B2

公开(公告)日：2016-02-02

申请号：US12509766

申请日：2009-09-09

申请人： Joseph F. Petolino ,  Qihua C. Cai ,  Weiting W. Ni

发明人： Joseph F. Petolino ,  Qihua C. Cai ,  Weiting W. Ni

IPC分类号： C12N15/09 ,  C12N15/82

CPC分类号： C12N15/8201

摘要： The present invention relates to methods and compositions for the expression of protein. Embodiments of methods may comprise the cleavage and repair of a nucleotide sequence encoding a highly expressed protein leading to a reduction in the expression of the highly expressed protein.

摘要翻译： 本发明涉及蛋白质表达的方法和组合物。 方法的实施方案可以包括编码高表达蛋白质的核苷酸序列的切割和修复，导致高度表达的蛋白质的表达降低。

公开(公告)号：US09187758B2

公开(公告)日：2015-11-17

申请号：US12001939

申请日：2007-12-13

申请人： Qihua C. Cai ,  Vipula K. Shukla ,  Joseph F. Petolino ,  Lisa W. Baker ,  Robbi J. Garrison ,  Ryan C. Blue ,  Jon C. Mitchell ,  Nicole L. Arnold ,  Sarah E. Worden ,  Jeffrey Miller ,  Fyodor Urnov

发明人： Qihua C. Cai ,  Vipula K. Shukla ,  Joseph F. Petolino ,  Lisa W. Baker ,  Robbi J. Garrison ,  Ryan C. Blue ,  Jon C. Mitchell ,  Nicole L. Arnold ,  Sarah E. Worden ,  Jeffrey Miller ,  Fyodor Urnov

IPC分类号： C12N15/82 ,  C07K19/00 ,  C12N15/62

CPC分类号： C07K14/4702 ,  C07K2319/81 ,  C12N15/8213 ,  C12N15/8243

摘要： Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.