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    EXCISION OF TRANSGENES IN GENETICALLY MODIFIED ORGANISMS
    1.
    发明申请
    EXCISION OF TRANSGENES IN GENETICALLY MODIFIED ORGANISMS 有权
    在遗传改良机构中实施转基因

    公开(公告)号：US20110191877A1

    公开(公告)日：2011-08-04

    申请号：US13011666

    申请日：2011-01-21

    申请人： Sean M. Russell ,  Joseph F. Petolino

    发明人： Sean M. Russell ,  Joseph F. Petolino

    IPC分类号： A01H1/00 ,  A01H1/06 ,  A01H5/00 ,  C07H21/00 ,  C12N5/10

    CPC分类号： C12N15/8213 ,  A01H1/02 ,  C12N9/22 ,  C12N15/8241 ,  C12N15/8265

    摘要： A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.

    摘要翻译： 一种删除植物中DNA区域的方法。 在一些实施方案中，所述方法包括用核酸分子转化植物，其中所述核酸分子编码与一个或多个组织特异性启动子可操作地连接的一个或多个锌指核酸酶（ZFN），例如， 花粉特异性启动子。 方法包括在植物中切除天然基因。 因此，在一些实施方案中，ZFN被设计为识别与天然植物基因相邻的序列。 在进一步的实施方案中，ZFN在发育阶段特异性启动子的控制下表达，使得例如在相对较晚的发育阶段在植物中特异性切除核酸序列。 包括用于实施所公开的方法的核酸分子和通过该方法生产的植物。

    Increasing the anther culturability of maize
    4.
    发明授权
    Increasing the anther culturability of maize 失效
    增加玉米的花药培养

    公开(公告)号：US5602310A

    公开(公告)日：1997-02-11

    申请号：US221297

    申请日：1994-03-31

    申请人： Joseph F. Petolino

    发明人： Joseph F. Petolino

    IPC分类号： A01H1/02 ,  A01H4/00 ,  A01H5/00 ,  A01H1/04 ,  C12N15/02

    CPC分类号： A01H4/00 ,  A01H1/02

    摘要： This invention provides a process for producing germplasm of plant species exhibiting enhanced response to anther culture. The anther culture procedure itself is used as a selection criterion for genes favoring in vitro androgenesis. After subjecting anthers to standard anther culture regeneration procedures, the regenerated plants are intermated and self-pollinated to generate valuable genetic variability for improved culture response. The transfer of increased anther culturability to other selected germplasm is also possible.

    摘要翻译： 本发明提供了一种用于生产对花药培养具有增强反应的植物物种的种质的方法。 花药培养程序本身被用作有利于体外雄激素发生的基因的选择标准。 在使花药进行标准的花药培养再生程序后，再生植物被间接和自花授粉以产生有价值的遗传变异性以改善培养反应。 将花药培养增加转移到其他选择的种质也是可能的。

    Increasing the anther culturability of maize
    8.
    发明授权
    Increasing the anther culturability of maize 失效
    增加玉米的花药培养

    公开(公告)号：US5306864A

    公开(公告)日：1994-04-26

    申请号：US839024

    申请日：1992-02-18

    申请人： Joseph F. Petolino

    发明人： Joseph F. Petolino

    IPC分类号： A01H1/02 ,  A01H4/00 ,  A01H5/00 ,  A01H1/04

    CPC分类号： A01H4/00 ,  A01H1/02

    摘要： This invention provides a process for producing germplasm of plant species exhibiting enhanced response to anther culture. The anther culture procedure itself is used as a selection criterion for genes favoring in vitro androgenesis. After subjecting anthers to standard anther culture regeneration procedures, the regenerated plants are intermated and self-pollinated to generate valuable genetic variability for improved culture response. The transfer of increased anther culturability to other selected germplasm is also possible.

    摘要翻译： 本发明提供了一种用于生产对花药培养具有增强反应的植物物种的种质的方法。 花药培养程序本身被用作有利于体外雄激素发生的基因的选择标准。 在使花药进行标准的花药培养再生程序后，再生植物被间接和自花授粉以产生有价值的遗传变异性以改善培养反应。 将花药培养增加转移到其他选择的种质也是可能的。

    Excision of transgenes in genetically modified organisms
    9.
    发明授权
    Excision of transgenes in genetically modified organisms 有权

    公开(公告)号：US09695432B2

    公开(公告)日：2017-07-04

    申请号：US13011666

    申请日：2011-01-21

    申请人： Sean M. Russell ,  Joseph F. Petolino

    发明人： Sean M. Russell ,  Joseph F. Petolino

    IPC分类号： C12N15/82 ,  C12N9/22 ,  A01H1/02

    CPC分类号： C12N15/8213 ,  A01H1/02 ,  C12N9/22 ,  C12N15/8241 ,  C12N15/8265

    摘要： A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.