公开(公告)号：US20050106606A1

公开(公告)日：2005-05-19

申请号：US10965018

申请日：2004-10-13

申请人： Hsing-Yeh Parker ,  John Mulligan

发明人： Hsing-Yeh Parker ,  John Mulligan

IPC分类号： C07H21/04 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12P19/34 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00308 ,  B01J2219/00351 ,  B01J2219/00353 ,  B01J2219/00389 ,  B01J2219/00495 ,  B01J2219/00547 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00698 ,  B01J2219/00722 ,  C07H21/00

摘要： Polynucleotides having in excess of 1,000 nucleotides can be prepared using a solid phase synthesis technique. A feature of the technique is the use of a reusable solid support that contains covalently bound oligonucleotide. This covalently bound oligonucleotide is annealed to a bridge oligonucleotide, where the bridge is also annealed to a first oligonucleotide that forms a portion of the target polynucleotide. After the target polynucleotide is synthesized, it can be removed from the solid support under denaturing conditions, and the solid support re-used to prepare additional target polynucleotides. The yield of the target polynucleotide increases when shearing force is applied to the solid support that is linked to the growing oligonucleotide. This shearing force is thought to extend the growing end of the oligonucleotide away from contact with other oligonucleotide bound to the solid support and make that end more accessible to annealing with solution oligonucleotide. The synthesis is conveniently accomplished on a porous frit, where reagents and washing solutions are pumped through the frit.

摘要翻译： 具有超过1,000个核苷酸的多核苷酸可以使用固相合成技术制备。 该技术的特征是使用含有共价结合的寡核苷酸的可重复使用的固体支持物。 将共价结合的寡核苷酸与桥寡核苷酸退火，其中桥还与形成靶多核苷酸的一部分的第一寡核苷酸退火。 在合成靶多核苷酸之后，可以在变性条件下将其从固体支持物上除去，并且固体支持物再次用于制备另外的靶多核苷酸。 当剪切力施加到与生长的寡核苷酸连接的固体支持物上时，靶多核苷酸的产量增加。 认为这种剪切力使得寡核苷酸的生长末端远离与与固体支持物结合的其它寡核苷酸的接触，并使得该结果更容易用溶液寡核苷酸退火。 合成方便地在多孔玻璃料上完成，其中试剂和洗涤溶液通过玻璃料泵送。

公开(公告)号：US20110256031A1

公开(公告)日：2011-10-20

申请号：US12932334

申请日：2011-02-22

申请人： Hsing-Yeh Parker ,  John C. Tabone ,  John Mulligan

发明人： Hsing-Yeh Parker ,  John C. Tabone ,  John Mulligan

IPC分类号： B01J19/00

CPC分类号： B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00315 ,  B01J2219/0036 ,  B01J2219/00414 ,  B01J2219/00423 ,  B01J2219/00722

摘要： Embodiments of the present invention are directed to automated-polymer-synthesis systems that include discrete reagent-solution-addition, wait-time, and reagent-solution-draining sub-systems which together significantly increase throughput and decrease sub-system idle time. The automated-polymer-synthesis systems that represent embodiments of the present invention additionally include switches at points in which carriers can be received from multiple input paths or output to multiple different output paths. The automated-polymer-synthesis systems that represent embodiments of the present invention generally include an input spur and output spur in addition to a main loop, allowing carriers containing only completed polymers to be removed and new carriers input, so that carriers traverse the automated-polymer-synthesis systems independently from one another.

摘要翻译： 本发明的实施方案涉及自动聚合物合成系统，其包括离散的试剂 - 溶液添加，等待时间和试剂 - 溶液 - 排出子系统，这些子系统共同显着增加生产量并减少子系统空闲时间。 代表本发明实施例的自动化 - 聚合物合成系统另外包括在多个输入路径上可以接收载波或输出到多个不同输出路径的点处的交换机。 代表本发明实施例的自动化 - 聚合物 - 合成系统通常包括除了主回路之外的输入支线和输出支线，允许仅去除已完成的聚合物的载流子和新的载流子输入，使得载流子穿过自动化 - 聚合物合成系统彼此独立。

公开(公告)号：US20110236270A1

公开(公告)日：2011-09-29

申请号：US12932337

申请日：2011-02-22

申请人： Hsing-Yeh Parker ,  John C. Tabone ,  John Mulligan

发明人： Hsing-Yeh Parker ,  John C. Tabone ,  John Mulligan

IPC分类号： B01J19/00

CPC分类号： B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00315 ,  B01J2219/0036 ,  B01J2219/00414 ,  B01J2219/00423 ,  B01J2219/00722

摘要： Embodiments of the present invention include processing steps and subsystems, within automated-biopolymer-synthesis systems and within other automated systems for organic-chemistry-based processing, for removing reagent solutions and solvents from reaction chambers following various synthetic reaction steps and washing steps undertaken during biopolymer synthesis. Embodiments of the present invention employ any of various different types of liquid-absorbing materials to wick, or remove by capillary action, liquids from reaction chambers. Wicking-based methods and subcomponents of the present invention remove significantly greater fractions of solutions from reaction chambers than conventional methods and subsystems and, in addition, are mechanically simpler and produce fewer deleterious side effects than currently used methods and subsystems.

摘要翻译： 本发明的实施方案包括在自动生物聚合物 - 合成系统内和在用于基于有机化学的处理的其他自动化系统内的处理步骤和子系统，用于在各种合成反应步骤之后从反应室中除去试剂溶液和溶剂，并在 生物聚合物合成。 本发明的实施例使用各种不同类型的液体吸收材料中的任何一种来吸收或者通过毛细管作用从反应室中除去液体。 与常规方法和子系统相比，本发明的基于芯吸的方法和子部件从常规方法和子系统中除去反应室的溶液的显着更多的部分，此外，与目前使用的方法和子系统相比，机械上更简单并且产生较少有害的副作用。

公开(公告)号：US20060233801A1

公开(公告)日：2006-10-19

申请号：US11399210

申请日：2006-04-05

申请人： Mary Brunkow ,  David Galas ,  Brian Kovacevich ,  John Mulligan ,  Bryan Paeper ,  Jeffrey Ness ,  David Winkler

发明人： Mary Brunkow ,  David Galas ,  Brian Kovacevich ,  John Mulligan ,  Bryan Paeper ,  Jeffrey Ness ,  David Winkler

IPC分类号： A61K39/395 ,  C07H21/04 ,  C12P21/06 ,  C07K16/26 ,  C07K16/28 ,  C12N5/06

CPC分类号： C07K16/22 ,  A01K2217/05 ,  A01K2217/075 ,  A61K48/00 ,  C07K14/51 ,  C07K16/18 ,  C07K2317/11 ,  C07K2317/23 ,  C07K2319/00 ,  C12N2799/021 ,  G01N33/6872 ,  G01N2333/495 ,  G01N2500/04 ,  G01N2800/108

摘要： A novel class or family of TGF-β binding proteins is disclosed. Also disclosed are assays for selecting molecules for increasing bone mineralization and methods for utilizing such molecules. In particular, compositions and methods relating to antibodies that specifically bind to TGF-beta binding proteins are provided. These methods and compositions relate to altering bone mineral density by interfering with the interaction between a TGF-beta binding protein sclerostin and a TGF-beta superfamily member, particularly a bone morphogenic protein. Increasing bone mineral density has uses in diseases and conditions in which low bone mineral density typifies the condition, such as osteopenia, osteoporosis, and bone fractures.

摘要翻译： 公开了一种新型的TGF-β结合蛋白类或家族。 还公开了用于选择用于增加骨矿化的分子的测定法和利用这种分子的方法。 特别地，提供了与特异性结合TGF-β结合蛋白的抗体相关的组合物和方法。 这些方法和组合物涉及通过干扰TGF-β结合蛋白质硬化蛋白和TGF-β超家族成员，特别是骨形态发生蛋白之间的相互作用来改变骨矿物质密度。 增加骨矿物质密度在低骨矿物质密度代表骨质减少症，骨质疏松症和骨折等疾病和病症中具有应用价值。

公开(公告)号：US20060057566A1

公开(公告)日：2006-03-16

申请号：US10465524

申请日：2003-06-19

申请人： Jeffrey Van Ness ,  John Tabone ,  J. Howbert ,  John Mulligan

发明人： Jeffrey Van Ness ,  John Tabone ,  J. Howbert ,  John Mulligan

IPC分类号： C12Q1/68 ,  C08G63/91

CPC分类号： C07H21/00 ,  C07B2200/11 ,  C12Q1/6825 ,  C12Q1/6846 ,  C12Q1/6872 ,  C40B40/00 ,  C12Q2563/149 ,  C12Q2563/113 ,  C12Q2563/167 ,  C12Q2525/191

摘要： Tags and linkers specifically designed for a wide variety of nucleic acid reactions are disclosed, which are suitable for a wide variety of nucleic acid reactions wherein separation of nucleic acid molecules based upon size is required.

摘要翻译： 公开了针对各种核酸反应专门设计的标签和接头，其适用于各种各样的核酸反应，其中需要基于尺寸分离核酸分子。

公开(公告)号：US09021290B2

公开(公告)日：2015-04-28

申请号：US13440829

申请日：2012-04-05

申请人： Robert J. Hueston ,  Julia D. Harper ,  John Mulligan ,  Michael Banatt

发明人： Robert J. Hueston ,  Julia D. Harper ,  John Mulligan ,  Michael Banatt

IPC分类号： G06F1/32

CPC分类号： G06F1/3206

摘要： A system and method of dynamically managing a power supply allocation for each one of the server blades in a blade server includes a blade server system having a blade chassis, multiple server blades coupled to the blade chassis, a power supply system coupled to the blade chassis, a chassis management module coupled to the blade chassis, wherein the blade chassis includes electrical and data communication interconnections between the server blades, the redundant power supply system and the chassis management module. The chassis management module includes computer readable media having program instructions for dynamically managing a power supply allocation for each one of the server blades.

摘要翻译： 动态管理刀片服务器中的每个服务器刀片的电源分配的系统和方法包括具有刀片机箱，耦合到刀片机箱的多个服务器刀片的刀片服务器系统，耦合到刀片机箱的电源系统 耦合到所述刀片机箱的机箱管理模块，其中所述刀片机箱包括所述服务器刀片之间的电和数据通信互连，所述冗余电源系统和所述机箱管理模块。 机箱管理模块包括具有用于动态管理每个服务器刀片的电源分配的程序指令的计算机可读介质。

公开(公告)号：US08580201B2

公开(公告)日：2013-11-12

申请号：US12932337

申请日：2011-02-22

申请人： Hsing-Yeh Parker ,  John C. Tabone ,  John Mulligan

发明人： Hsing-Yeh Parker ,  John C. Tabone ,  John Mulligan

IPC分类号： B01J19/00 ,  B01J8/00 ,  B01J8/04 ,  C12Q1/68 ,  G01N31/00 ,  G01N33/00 ,  G01N31/22 ,  G01N33/52 ,  B01L3/00 ,  B01L99/00 ,  B01L3/02 ,  F04D19/00 ,  B01D21/00

CPC分类号： B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00315 ,  B01J2219/0036 ,  B01J2219/00414 ,  B01J2219/00423 ,  B01J2219/00722

摘要： Embodiments of the present invention include processing steps and subsystems, within automated-biopolymer-synthesis systems and within other automated systems for organic-chemistry-based processing, for removing reagent solutions and solvents from reaction chambers following various synthetic reaction steps and washing steps undertaken during biopolymer synthesis. Embodiments of the present invention employ any of various different types of liquid-absorbing materials to wick, or remove by capillary action, liquids from reaction chambers. Wicking-based methods and subcomponents of the present invention remove significantly greater fractions of solutions from reaction chambers than conventional methods and subsystems and, in addition, are mechanically simpler and produce fewer deleterious side effects than currently used methods and subsystems.

摘要翻译： 本发明的实施方案包括在自动生物聚合物 - 合成系统内和在用于基于有机化学的处理的其他自动化系统内的处理步骤和子系统，用于在各种合成反应步骤之后从反应室中除去试剂溶液和溶剂，并在 生物聚合物合成。 本发明的实施例使用各种不同类型的液体吸收材料中的任何一种来吸收或者通过毛细管作用从反应室中除去液体。 与常规方法和子系统相比，本发明的基于芯吸的方法和子部件从常规方法和子系统中除去反应室的溶液的显着更多的部分，此外，与目前使用的方法和子系统相比，机械上更简单并且产生较少有害的副作用。

公开(公告)号：US20080009613A1

公开(公告)日：2008-01-10

申请号：US11821531

申请日：2007-06-21

申请人： Jeffrey Van Ness ,  John Tabone ,  J. Howbert ,  John Mulligan

发明人： Jeffrey Van Ness ,  John Tabone ,  J. Howbert ,  John Mulligan

IPC分类号： C07C229/00 ,  C07C205/00 ,  C07D211/30 ,  C07H21/04

CPC分类号： C07H21/00 ,  C07B2200/11 ,  C07D211/78 ,  C07H21/04 ,  C12Q1/6872 ,  C12Q1/6874 ,  C40B40/00 ,  Y10T436/24 ,  C12Q2565/629 ,  C12Q2563/167

摘要： Methods and compounds, including compositions therefrom, are provided for determining the sequence of nucleic acid molecules. The methods permit the determination of multiple nucleic acid sequences simultaneously. The compounds are used as tags to generate tagged nucleic acid fragments which are complementary to a selected target nucleic acid molecule. Each tag is correlative with a particular nucleotide and, in a preferred embodiment, is detectable by mass spectrometry. Following separation of the tagged fragments by sequential length, the tags are cleaved from the tagged fragments. In a preferred embodiment, the tags are detected by mass spectrometry and the sequence of the nucleic acid molecule is determined therefrom. The individual steps of the methods can be used in automated format, e.g., by the incorporation into systems.

摘要翻译： 提供方法和化合物，包括其组合物，用于确定核酸分子的序列。 该方法允许同时测定多个核酸序列。 该化合物用作标签以产生与选定的靶核酸分子互补的标记的核酸片段。 每个标签与特定核苷酸相关，并且在优选实施方案中可通过质谱法检测。 在通过连续长度分离标记的片段之后，从标记的片段切割标签。 在优选的实施方案中，通过质谱检测标签，并由此确定核酸分子的序列。 方法的各个步骤可以以自动格式使用，例如通过并入系统。

公开(公告)号：US20050069928A1

公开(公告)日：2005-03-31

申请号：US10911239

申请日：2004-08-03

申请人： Jeffrey Nelson ,  John Mulligan ,  John Tabone

发明人： Jeffrey Nelson ,  John Mulligan ,  John Tabone

IPC分类号： A61K48/00 ,  C07H21/04 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12N15/1031

摘要： Disclosed is a significantly improved synthetic method of producing a set of mutagenized progeny polynucleotides which contain at least one substituted codon encoding for each of the 20 naturally encoded amino acids or any selected subset thereof. This in turn, similarly provides a method for producing from a parental template polypeptide, a set of mutagenized progeny polypeptides in which all 20 naturally encoded amino acids is represented at each original amino acid position or any selected subset thereof. The methods described herein enable the synthesis of defined, complex mixtures of oligonucleotides, in instances where the incorporation of degenerate bases is impractical. These oligonucleotide mixtures are useful for a variety of applications such as recombination methods, site-saturation mutagenesis, or the like.

摘要翻译： 公开了一种显着改进的合成方法，其生产一组诱变的后代多核苷酸，其含有至少一个编码20个天然编码氨基酸或其任何选定子集中的每一个的取代密码子。 这反过来类似地提供了从亲本模板多肽，一组诱变的后代多肽产生的方法，其中在每个原始氨基酸位置或其任何选定的亚组中表示所有20个天然编码的氨基酸。 在简并碱基的掺入是不实际的情况下，本文描述的方法能够合成寡核苷酸的限定的复杂混合物。 这些寡核苷酸混合物可用于各种应用，例如重组方法，位点饱和诱变等。