公开(公告)号：US07439051B2

公开(公告)日：2008-10-21

申请号：US10795676

申请日：2004-03-08

申请人： Alexander V. Sokoloff ,  Jon A. Wolff

发明人： Alexander V. Sokoloff ,  Jon A. Wolff

IPC分类号： C12N7/00 ,  C12N15/00 ,  C12Q1/70

CPC分类号： G01N33/6878 ,  G01N33/543

摘要： The present invention relates to compositions and methods for the selection and use of surface exposed epitopes. The present invention includes in vivo and in vitro phage peptide diplay methods for the identification and selection of peptides and peptide associated factors with desired properties (e.g., targeting specificity, stability, etc.). The present invention further provides methods and compositions for the isolation and identification of peptide-specific antibodies. The present invention also includes methods and compositions employing nuclear localization signals for enhanced nuclear transport and expression of DNA.

摘要翻译： 本发明涉及用于选择和使用表面暴露表位的组合物和方法。 本发明包括用于鉴定和选择具有所需性质（例如，靶向特异性，稳定性等）的肽和肽相关因子的体内和体外噬菌体肽双重显示方法。 本发明还提供了用于分离和鉴定肽特异性抗体的方法和组合物。 本发明还包括使用核定位信号用于增强核转运和DNA表达的方法和组合物。

公开(公告)号：US07148205B2

公开(公告)日：2006-12-12

申请号：US10600290

申请日：2003-06-20

申请人： Sean D. Monahan ,  Jon A. Wolff ,  Paul M. Slattum ,  James E. Hagstrom ,  Vladimir G. Budker ,  David B. Rozema

发明人： Sean D. Monahan ,  Jon A. Wolff ,  Paul M. Slattum ,  James E. Hagstrom ,  Vladimir G. Budker ,  David B. Rozema

IPC分类号： A61K31/70 ,  C07H21/04

CPC分类号： C12N15/87 ,  A61K31/70 ,  A61K47/59 ,  A61K47/593 ,  A61K47/645 ,  A61K48/00 ,  A61K48/0083 ,  C07H21/00 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/3233 ,  C12N2320/32 ,  A61K2300/00

摘要： Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.

公开(公告)号：US07087770B2

公开(公告)日：2006-08-08

申请号：US09779791

申请日：2001-02-08

申请人： Jon A. Wolff ,  Sean D. Monahan ,  Vladimir G. Budker ,  Paul M. Slattum ,  David B. Rozema

发明人： Jon A. Wolff ,  Sean D. Monahan ,  Vladimir G. Budker ,  Paul M. Slattum ,  David B. Rozema

IPC分类号： C11D1/60 ,  A61K9/127 ,  C12N15/88

CPC分类号： A61K48/00 ,  A61K31/155 ,  A61K31/198 ,  A61K38/08 ,  C07C323/60 ,  C07C323/62 ,  C07D207/46 ,  C07H21/04

摘要： A labile disulfide-containing compound under physiological conditions containing a labile disulfide bond and a transduction signal.

摘要翻译： 含有不稳定二硫键和转导信号的生理条件下的不稳定二硫键化合物。

公开(公告)号：US07045356B2

公开(公告)日：2006-05-16

申请号：US10795679

申请日：2004-03-08

申请人： Vladimir Trubetskoy ,  Jon A. Wolff ,  James E. Hagstrom ,  Vladimir G. Budker ,  So Chun Wong ,  Jason Klein

发明人： Vladimir Trubetskoy ,  Jon A. Wolff ,  James E. Hagstrom ,  Vladimir G. Budker ,  So Chun Wong ,  Jason Klein

IPC分类号： C12N15/88

CPC分类号： A61K48/0008

摘要： We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.

摘要翻译： 我们描述了一种产生多层颗粒的方法，包括将聚合物与带相反电荷的聚合物缩合形成颗粒，并依次向形成至少三层聚合物的颗粒中加入带相反电荷的聚合物。 该方法用于形成用于将生物活性化合物递送至细胞的组合物。

公开(公告)号：US07022525B2

公开(公告)日：2006-04-04

申请号：US10755785

申请日：2004-01-12

申请人： Vladimir Trubetskoy ,  Jon A. Wolff ,  Paul M. Slattum ,  Lisa Hanson ,  Vladimir G. Budker ,  James E. Hagstrom

发明人： Vladimir Trubetskoy ,  Jon A. Wolff ,  Paul M. Slattum ,  Lisa Hanson ,  Vladimir G. Budker ,  James E. Hagstrom

IPC分类号： C12N15/09 ,  C12N15/00 ,  C12N15/11 ,  A61K48/00 ,  A61K39/395

CPC分类号： C12N15/87 ,  A61K47/59 ,  A61K47/60 ,  A61K47/645 ,  A61K48/00 ,  A61K48/0008 ,  A61K48/0025 ,  A61K48/0041

摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.

公开(公告)号：US06818626B1

公开(公告)日：2004-11-16

申请号：US09234606

申请日：1999-01-21

申请人： Jon A. Wolff ,  James E. Hagstrom ,  Sean D. Monahan ,  Vladimir Budker ,  Vladimir Trubetskoy ,  Paul M. Slattum

发明人： Jon A. Wolff ,  James E. Hagstrom ,  Sean D. Monahan ,  Vladimir Budker ,  Vladimir Trubetskoy ,  Paul M. Slattum

IPC分类号： A61K4800

CPC分类号： A61K48/0025 ,  A61K48/00 ,  C12N15/87

摘要： Chelator containing compounds are utilized in the delivery of molecules, polymers, nucleic acids and genes to animal cells. At least one chelator such as crown ether is attached to a polymer and then associated with another polymer such as DNA. An ion is then added to the mixture thereby forming condensed DNA. In condensed form and in complex with the chelator, DNA can be delivered to a cell.

公开(公告)号：US06379966B2

公开(公告)日：2002-04-30

申请号：US09450315

申请日：1999-11-29

申请人： Sean D. Monahan ,  Jon A. Wolff ,  Paul M. Slattum ,  James E. Hagstrom ,  Vladimir G. Budker ,  David B. Rozema

发明人： Sean D. Monahan ,  Jon A. Wolff ,  Paul M. Slattum ,  James E. Hagstrom ,  Vladimir G. Budker ,  David B. Rozema

IPC分类号： C12N1585

CPC分类号： A61K48/0041 ,  A61K48/0008 ,  A61K48/0083

摘要： Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.

摘要翻译： 公开了用于在细胞中提供核酸表达的复合物。 将多核苷酸和聚合物混合在一起形成复合物，其中络合物的ζ电位不是正的。 然后将复合物递送到其中表达核酸的细胞。

公开(公告)号：US6126964A

公开(公告)日：2000-10-03

申请号：US778657

申请日：1997-01-03

申请人： Jon A. Wolff ,  James E. Hagstrom ,  Vladimir G. Budker ,  Vladimir S. Trubetskoy ,  Paul M. Slattum ,  Lisa J. Hanson

发明人： Jon A. Wolff ,  James E. Hagstrom ,  Vladimir G. Budker ,  Vladimir S. Trubetskoy ,  Paul M. Slattum ,  Lisa J. Hanson

IPC分类号： A61K47/48 ,  A61K48/00 ,  C12N15/87 ,  A61K9/127 ,  C12N15/64

CPC分类号： A61K47/48315 ,  A61K47/48192 ,  A61K47/48215 ,  A61K48/00 ,  A61K48/0008 ,  A61K48/0025 ,  A61K48/0041 ,  C12N15/87

摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.

公开(公告)号：US20120172412A1

公开(公告)日：2012-07-05

申请号：US13336028

申请日：2011-12-23

申请人： David B. Rozema ,  Darren H. Wakefield ,  David L. Lewis ,  Jon A. Wolff ,  Andrei V. Blokhin ,  Jonathan D. Benson ,  Jeffrey C. Carlson ,  Philipp Hadwiger ,  Eric A. Kitas ,  Torsten Hoffmann ,  Kerstin Jahn-Hofmann ,  Peter Mohr ,  Hans Martin Mueller ,  Guenther Ott ,  Ingo Roehl

发明人： David B. Rozema ,  Darren H. Wakefield ,  David L. Lewis ,  Jon A. Wolff ,  Andrei V. Blokhin ,  Jonathan D. Benson ,  Jeffrey C. Carlson ,  Philipp Hadwiger ,  Eric A. Kitas ,  Torsten Hoffmann ,  Kerstin Jahn-Hofmann ,  Peter Mohr ,  Hans Martin Mueller ,  Guenther Ott ,  Ingo Roehl

IPC分类号： A61K48/00 ,  C08G63/91 ,  C07C69/96

CPC分类号： A61K38/00 ,  A61K31/7088 ,  A61K47/60 ,  A61K47/65 ,  A61K48/00 ,  C07C69/96 ,  C07C271/20 ,  C07H15/04 ,  C07H15/20 ,  C07H15/26 ,  C07K7/02 ,  C07K2317/77 ,  C12N15/1138 ,  C12N15/87 ,  C12N2310/14 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/351 ,  C12N2320/30

摘要： The present invention is directed compositions for delivery of RNA interference (RNAi) polynucleotides to cells in vivo. The compositions comprise amphipathic membrane active polyamines reversibly modified with enzyme cleavable dipeptide-amidobenzyl-carbonate masking agents. Modification masks membrane activity of the polymer while reversibility provides physiological responsiveness. The reversibly modified polyamines (dynamic polyconjugate or DPC) are further covalently linked to an RNAi polynucleotide or co-administered with a targeted RNAi polynucleotide-targeting molecule conjugate.

摘要翻译： 本发明涉及用于将RNA干扰（RNAi）多核苷酸递送至细胞体内的组合物。 组合物包含用酶切割二肽 - 氨基苄基 - 碳酸酯掩蔽剂可逆地修饰的两亲膜活性多胺。 改性掩盖聚合物的膜活性，而可逆性提供生理反应性。 可逆修饰的多胺（动态多偶联物或DPC）进一步共价连接到RNAi多核苷酸或与靶向RNAi多核苷酸靶向分子缀合物共同施用。

公开(公告)号：US07524680B2

公开(公告)日：2009-04-28

申请号：US10095681

申请日：2002-03-11

申请人： Jon A. Wolff ,  James E. Hagstrom ,  Vladimir G. Budker ,  Vladimir S. Trubetskoy

发明人： Jon A. Wolff ,  James E. Hagstrom ,  Vladimir G. Budker ,  Vladimir S. Trubetskoy

IPC分类号： C12N15/88

CPC分类号： C12N15/88 ,  A61K47/58 ,  A61K47/59 ,  A61K47/60 ,  A61K47/645 ,  A61K48/00 ,  A61K48/0008 ,  A61K48/0025 ,  A61K48/0041 ,  C12N15/87

摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.