公开(公告)号：US20160237462A1

公开(公告)日：2016-08-18

申请号：US14437672

申请日：2013-10-22

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO ,  Keiji MATSUMOTO

IPC: C12P7/62 ,  C12N15/74

CPC classification number: C12P7/625 ,  C12N9/18 ,  C12N15/74

Abstract: An object of the present invention is to provide a microorganism strain that accumulates a high molecular weight PHA, and a PHA production method using the microorganism. The present invention provides a method for producing a PHA copolymer, which includes culturing a microorganism, wherein at least a portion of either of the following genes (a) and (b) of the microorganism has been altered by substitution, deletion, insertion, and/or addition to reduce or eliminate the activity of a PHA degrading enzyme encoded by the gene: (a) a PHA degrading enzyme gene encoding the amino acid sequence of SEQ ID NO:2 in the sequence listing; and (b) a gene encoding a polypeptide having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:2 in the sequence listing and having PHA degrading enzyme activity.

Abstract translation: 本发明的目的是提供一种积累高分子量PHA的微生物菌株和使用该微生物的PHA生产方法。 本发明提供一种生产PHA共聚物的方法，其包括培养微生物，其中微生物的以下基因（a）和（b）中的至少一部分已经被替换，缺失，插入和 /或添加以减少或消除由该基因编码的PHA降解酶的活性：（a）编码序列表中SEQ ID NO：2的氨基酸序列的PHA降解酶基因; 和（b）编码与序列表中SEQ ID NO：2的氨基酸序列具有至少85％序列同一性且具有PHA降解酶活性的多肽的基因。

公开(公告)号：US20240417742A1

公开(公告)日：2024-12-19

申请号：US18695099

申请日：2022-09-28

Applicant: KANEKA CORPORATION

Inventor： Ken HARADA ,  Shunsuke SATO ,  Hisashi ARIKAWA

IPC: C12N15/74 ,  C12N1/20 ,  C12N9/02 ,  C12N9/10 ,  C12P7/625

Abstract: A microorganism is cultivated in the presence of a carbon source. The microorganism is a transformed microorganism having an introduced fadH gene encoding 2,4-dienoyl-CoA reductase. That is, the transformed microorganism has an introduced foreign gene encoding a protein having an amino acid sequence of SEQ ID NO: 1 or an introduced foreign gene encoding a protein having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 1. The carbon source contains an oil containing unsaturated fatty acids as constituents or free unsaturated fatty acids.

公开(公告)号：US20210254028A1

公开(公告)日：2021-08-19

申请号：US17252086

申请日：2019-06-12

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Shunsuke SATO ,  Naoaki TAOKA

IPC: C12N9/10 ,  C12N15/52 ,  C12P7/62

Abstract: Provided is a mutant polyhydroxyalkanoate synthetase having an amino acid sequence which has 85% or more sequence identity with the amino acid sequence of SEQ ID NO: 1 and which contains a mutation of substitution of at least one of the amino acids at the 27th to 33rd, 39th, 56th, 106th, 129th, 144th, 165th, 170th and 172nd to 187th positions from the N-terminus of the amino acid sequence of SEQ ID NO: 1 with another amino acid.