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11.发明申请METHOD, REAGENT AND KIT FOR DETERMINATION OF CHOLESTEROL IN REMNANT-LIKE PARTICLES (RLP) 有权方法，试剂和试剂盒，用于确定残留样颗粒中的胆固醇（RLP）公开(公告)号：US20090023167A1
公开(公告)日：2009-01-22
申请号：US11815225
申请日：2006-02-14
申请人： Kazuhito Miyauchi , Mayumi Fujinaka
发明人： Kazuhito Miyauchi , Mayumi Fujinaka
IPC分类号： C12Q1/60
摘要： The present invention provides a method, a reagent and a kit for simple and sensitive determination of cholesterol in remnant-like particles without separation of components of a sample. A method for quantitatively determining remnant-like particle cholesterol in a sample, which comprises: in an aqueous medium containing the sample and in the presence of a combination of specific surfactants and a phospholipid-hydrolyzing enzyme, allowing remnant-like particle cholesterol in the sample to react with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase (in the presence of oxidized coenzyme); and determining the formed hydrogen peroxide or reduced coenzyme.
摘要翻译： 本发明提供了用于简单且灵敏地测定残留样颗粒中胆固醇而不分离样品组分的方法，试剂和试剂盒。 一种用于定量测定样品中残留样颗粒胆固醇的方法，其包括：在含有样品的水性介质中，并且在特定表面活性剂和磷脂水解酶的组合存在下，允许样品中的残留物颗粒胆固醇 与胆固醇酯酶和胆固醇氧化酶或胆固醇脱氢酶（在氧化型辅酶存在下）反应; 并测定形成的过氧化氢或还原型辅酶。
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12.发明申请Method, reagent and kit for determination of cholesterol in very low-density lipoprotein remnant (vldl remnant) 审中-公开用于测定非常低密度脂蛋白残留物（vldl残留物）中胆固醇的方法，试剂和试剂盒公开(公告)号：US20070161068A1
公开(公告)日：2007-07-12
申请号：US10586868
申请日：2005-01-28
申请人： Kazuhito Miyauchi
发明人： Kazuhito Miyauchi
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60 , C12Q1/34 , G01N33/92 , G01N2800/044
摘要： According to the present invention, esterified and free cholesterol in very low-density lipoprotein remnant (hereinafter collectively referred to as very low-density lipoprotein remnant cholesterol) in a sample is determined in an aqueous medium containing the sample in the presence of a combination of (a) a polyoxyethylene-polyoxyalkylene alkylaryl ether and (b) one kind of surfactant selected from the group consisting of a polyoxyethylene-polyoxybutylene copolymer, a polyoxyethylene styrenated-phenyl ether and a polyoxyalkylene long-chain alkyl ether which combination is capable of causing cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase to act specifically on very low-density lipoprotein remnant cholesterol.
摘要翻译： 根据本发明，在包含样品的水性介质中，在存在以下组合的情况下，测定样品中极低密度脂蛋白残留物（以下统称为极低密度脂蛋白残留胆固醇）中的酯化游离胆固醇 （a）聚氧乙烯 - 聚氧化烯烷基芳基醚和（b）一种选自聚氧乙烯 - 聚氧丁烯共聚物，聚氧乙烯苯乙烯基苯基醚和聚氧化烯长链烷基醚的表面活性剂，其组合能够引起胆固醇 酯酶和胆固醇氧化酶或胆固醇脱氢酶作用于非常低密度脂蛋白残留胆固醇。
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公开(公告)号：US09360432B2
公开(公告)日：2016-06-07
申请号：US12090906
申请日：2006-10-31
CPC分类号： G01N21/78 , C12Q1/44 , C12Q1/61 , G01N33/92 , G01N2333/91215 , G01N2333/918
摘要： The present invention provides a method for a simple and accurate measurement of triglycerides in low-density lipoprotein in a sample comprising performing the following steps sequentially:(i) a step of generating free glycerol by allowing lipoprotein lipase to act on a sample, in an aqueous medium comprising the sample and a specific surfactant such as polyoxyethylene polyoxyalkylene polycyclic phenyl ether; (ii) a step of removing free glycerol present in the reaction solution of the above step (i); (iii) a step of generating free glycerol by allowing lipoprotein lipase to act on the reaction solution from which free glycerol has been removed in step (ii), in the presence of a specific surfactant such as polyoxyethylene polyoxyalkylene alkyl ether; and (iv) a step of measuring free glycerol generated in step (iii), and a kit used for the method.
摘要翻译： 本发明提供了一种用于简单且准确地测量样品中低密度脂蛋白中的甘油三酸酯的方法，包括依次执行以下步骤：（i）通过使脂蛋白脂肪酶作用于样品，产生游离甘油的步骤， 包含样品的水性介质和特定表面活性剂如聚氧乙烯聚氧化烯多环苯基醚; （ii）除去上述步骤（i）的反应溶液中存在的游离甘油的步骤; （iii）在特定表面活性剂如聚氧乙烯聚氧化烯烷基醚的存在下，通过使脂蛋白脂肪酶作用于在步骤（ii）中除去游离甘油的反应溶液中，产生游离甘油的步骤; 和（iv）测量步骤（iii）中产生的游离甘油的步骤和用于该方法的试剂盒。
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14.发明申请METHOD FOR MEASURING TRIGLYCERIDE IN LOW-DENSITY LIPOPROTEIN AND KIT FOR MEASUREMENT 有权用于测量低密度脂蛋白中的TRIGLYCERIDE的方法和用于测量的试剂盒公开(公告)号：US20090226944A1
公开(公告)日：2009-09-10
申请号：US12090906
申请日：2006-10-31
IPC分类号： C12Q1/60
CPC分类号： G01N21/78 , C12Q1/44 , C12Q1/61 , G01N33/92 , G01N2333/91215 , G01N2333/918
摘要： The present invention provides a method for a simple and accurate measurement of triglycerides in low-density lipoprotein in a sample comprising performing the following steps sequentially:(i) a step of generating free glycerol by allowing lipoprotein lipase to act on a sample, in an aqueous medium comprising the sample and a specific surfactant such as polyoxyethylene polyoxyalkylene polycyclic phenyl ether; (ii) a step of removing free glycerol present in the reaction solution of the above step (i); (iii) a step of generating free glycerol by allowing lipoprotein lipase to act on the reaction solution from which free glycerol has been removed in step (ii), in the presence of a specific surfactant such as polyoxyethylene polyoxyalkylene alkyl ether; and (iv) a step of measuring free glycerol generated in step (iii), and a kit used for the method.
摘要翻译： 本发明提供了一种用于简单且准确地测量样品中低密度脂蛋白中的甘油三酸酯的方法，包括依次执行以下步骤：（i）通过使脂蛋白脂肪酶作用于样品，产生游离甘油的步骤， 包含样品的水性介质和特定表面活性剂如聚氧乙烯聚氧化烯多环苯基醚; （ii）除去上述步骤（i）的反应溶液中存在的游离甘油的步骤; （iii）在特定表面活性剂如聚氧乙烯聚氧化烯烷基醚的存在下，通过使脂蛋白脂肪酶作用于在步骤（ii）中除去游离甘油的反应溶液中，产生游离甘油的步骤; 和（iv）测量步骤（iii）中产生的游离甘油的步骤和用于该方法的试剂盒。
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15.发明申请Inhibitor for adhesion of metal hydroxide-containing substance to waste liquor line of automatic analyzer 审中-公开含有金属氢氧化物的物质与自动分析仪废液管道粘合的抑制剂公开(公告)号：US20050130323A1
公开(公告)日：2005-06-16
申请号：US10504581
申请日：2003-02-17
申请人： Kazuhito Miyauchi
发明人： Kazuhito Miyauchi
CPC分类号： C23F15/005 , C02F5/10 , G01N2035/00277
摘要： The present invention provides an inhibitor for adhesion of a metal hydroxide-containing substance to a waste liquid line of an autoanalyzer comprising a polysaccharide; a method of inhibiting adhesion of a metal hydroxide-containing substance to a waste liquid line of an autoanalyzer wherein the inhibitor is used; a reagent for measuring a biological component in a sample, which is effective for inhibiting adhesion of a metal hydroxide-containing substance to a waste liquid line, said reagent containing the said inhibitor; and a method for measuring a biological component in a sample wherein the reagent for measuring a biological component is used.
摘要翻译： 本发明提供了一种用于将含金属氢氧化物的物质粘附到包含多糖的自动分析仪的废液管线上的抑制剂; 一种抑制含金属氢氧化物的物质与使用抑制剂的自动分析仪的废液管线粘附的方法; 用于测量样品中的生物成分的试剂，其有效地抑制含金属氢氧化物的物质与废液管线的粘附，所述试剂含有所述抑制剂; 以及用于测量样品中的生物成分的方法，其中使用了用于测量生物成分的试剂。
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公开(公告)号：US06811994B1
公开(公告)日：2004-11-02
申请号：US09889742
申请日：2001-07-20
IPC分类号： C12Q134
CPC分类号： C12Q1/61 , C12Q1/44 , G01N2333/904 , G01N2333/908 , G01N2333/918
摘要： The present invention relates to a method for conveniently quantitating TGs contained in various lipoproteins. A method for quantitating trigyceride (TG) in a particular lipoprotein which comprises eliminating free glycerol from a sample containing free glycerol and TG in the particular lipoprotein, then allowing the resulting sample to react with lipoprotein lipase and an enzyme system which generates hydrogen peroxide from free glycerol, and quantitating the generated hydrogen peroxide, is provided.
摘要翻译： 本发明涉及方便地定量各种脂蛋白中含有的TG的方法。 一种在特定脂蛋白中定量三环霉素（TG）的方法，其包括从含有游离甘油和特定脂蛋白中的TG的样品中除去游离甘油，然后使得到的样品与脂蛋白脂肪酶和从游离体产生过氧化氢的酶系统反应 甘油，并定量生成的过氧化氢。
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公开(公告)号：US5807696A
公开(公告)日：1998-09-15
申请号：US737504
申请日：1996-11-13
申请人： Kazuhito Miyauchi , Akira Miike
发明人： Kazuhito Miyauchi , Akira Miike
CPC分类号： G01N33/92 , Y10S435/975
摘要： The present invention relates to a method for the determination of cholesterol in low-density lipoprotein (LDL) in a sample containing LDL, which comprises eliminating cholesterol in high-density lipoprotein in the sample, subjecting the sample to a reaction utilizing the action of a cholesterol ester-hydrolyzing enzyme and the action of a cholesterol-oxidizing enzyme or of cholesterol oxidoreductase, and determining the amount of hydrogen peroxide or a reduced type coenzyme generated by the reaction.
摘要翻译： PCT No.PCT / JP96 / 00664 Sec。 371日期：1996年11月13日 102（e）1996年11月13日PCT 1996年3月15日PCT公布。 公开号WO96 / 28734 日期1996年9月19日本发明涉及含有LDL的样品中低密度脂蛋白（LDL）中胆固醇的测定方法，该方法包括消除样品中高密度脂蛋白中的胆固醇，使样品进行反应 利用胆固醇酯水解酶的作用和胆固醇氧化酶或胆固醇氧化还原酶的作用，并测定通过反应产生的过氧化氢或还原型辅酶的量。
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