公开(公告)号：US09663554B2

公开(公告)日：2017-05-30

申请号：US14857661

申请日：2015-09-17

Applicant: LI-COR, INC.

Inventor： Nisha Padhye ,  Rose Skopp ,  Ying Wang

IPC: G01N33/58 ,  G01N33/96 ,  C07K7/06 ,  C07K14/795 ,  C12Q1/28

CPC classification number: G01N33/96 ,  C07K7/06 ,  C07K14/795 ,  C12Q1/28 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/908 ,  G01N2550/00

Abstract: Multifunctional molecular weight protein ladders and methods of making thereof are disclosed herein that are useful for determining the molecular weight of a test protein and/or the relative mass or amount of the test protein in a protein separation assay, such as gel electrophoresis or western blotting. Also included are compounds of Formula I (e.g., mono acetylated MP-11 NHS ester) that may be used to label purified proteins of the protein ladder. The MP-11 label protein ladder can be detected on a blotting membrane by exposing the microperoxidase to a suitable substrate, such as a chromogenic substrate or a chemiluminescent substrate.

公开(公告)号：US20140213473A1

公开(公告)日：2014-07-31

申请号：US14192827

申请日：2014-02-27

Applicant: LI-COR, INC.

Inventor： Kousik Kundu ,  Nisha Padhye ,  William Volcheck

IPC: G01N33/58

CPC classification number: C09B23/164 ,  C07D209/08 ,  C09B23/0008 ,  C09B23/0025 ,  C09B23/0066 ,  C09B23/083 ,  C09B23/086 ,  C09B23/107 ,  G01N33/574 ,  G01N33/582

Abstract: The present invention provides novel compounds and methods for hydrocyanines derived from near-infrared cyanine dyes, as reactive oxygen species probes in imaging. In certain embodiments, the present invention provides reduced dyes as substrates for ELISA and Western blots.

Abstract translation: 本发明提供了新型化合物和衍生自近红外花青染料的氢氰酸的新型化合物和方法，作为成像中的活性氧探针。 在某些实施方案中，本发明提供还原染料作为用于ELISA和Western印迹的底物。

公开(公告)号：US20170219613A1

公开(公告)日：2017-08-03

申请号：US15491905

申请日：2017-04-19

Applicant: LI-COR, Inc.

Inventor： Nisha Padhye ,  Rose Skopp ,  Ying Wang

IPC: G01N33/96 ,  G01N33/58 ,  C07K7/06

CPC classification number: G01N33/96 ,  C07K7/06 ,  C07K14/795 ,  C12Q1/28 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/908 ,  G01N2550/00

Abstract: Multifunctional molecular weight protein ladders and methods of making thereof are disclosed herein that are useful for determining the molecular weight of a test protein and/or the relative mass or amount of the test protein in a protein separation assay, such as gel electrophoresis or western blotting. Also included are compounds of Formula I (e.g., mono acetylated MP-11 NHS ester) that may be used to label purified proteins of the protein ladder. The MP-11 label protein ladder can be detected on a blotting membrane by exposing the microperoxidase to a suitable substrate, such as a chromogenic substrate or a chemiluminescent substrate.

公开(公告)号：US09632034B2

公开(公告)日：2017-04-25

申请号：US15068373

申请日：2016-03-11

Applicant: LI-COR, Inc.

Inventor： Ying Wang ,  Xinshe Xu ,  Nisha Padhye

IPC: G01N21/78 ,  C12Q1/28 ,  G01N33/58 ,  G01N21/76

CPC classification number: G01N21/78 ,  C12Q1/28 ,  G01N21/76 ,  G01N33/582

Abstract: The present invention provides near-infrared fluorogenic and/or chromogenic formulations, methods, systems and kits. Upon contacting an azine with an oxidant and a peroxidase, the azine is converted to an azine derivative which is both visibly colored and fluorescent. This invention also provides high sensitive methods to detect biological molecules, showing better sensitivity than chemiluminescence methods. Advantageously, the formulation and reaction is free of luminol or luminol derivatives.

公开(公告)号：US20150267108A1

公开(公告)日：2015-09-24

申请号：US14262659

申请日：2014-04-25

Applicant: LI-COR, INC.

Inventor： Xinshe Xu ,  Rose Skopp ,  Mark Cradduck ,  Nisha Padhye

IPC: C09K11/07 ,  B43K1/12 ,  B43K23/12 ,  F21K2/06

CPC classification number: C09K11/07 ,  B43K1/12 ,  C09K2211/1029 ,  C09K2211/188 ,  F21K2/06

Abstract: The present invention provides a chemiluminescent ink formulation, comprising: a phthalocyanine metal catalyst; a visible dye; and a solvent. The formulation is useful in catalyzing a chemiluminescent reaction, by admixing for example, luminol or isoluminol with an oxidizing agent, a base and the chemiluminescent ink formulation to emit light.

Abstract translation: 本发明提供一种化学发光油墨制剂，其包含：酞菁金属催化剂; 可见染料; 和溶剂。 该制剂可用于催化化学发光反应，例如通过将鲁米诺或异鲁米诺与氧化剂，碱和化学发光油墨制剂混合以发光。