公开(公告)号：US20140067345A1

公开(公告)日：2014-03-06

申请号：US14011190

申请日：2013-08-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Francis T. Cheng ,  Casey R. McFarland

IPC: G06F17/50

CPC classification number: G06F17/5009 ,  C12Q1/6816 ,  G06F19/18 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

Abstract: Methods are provided that operate on raw dissociation data and dissociation curves to generate calibrations of the detected data and to further improve analysis of the data. The data can be taken from each support region of a multi-region platform, for example, from each well of a multi-well plate. Each support region can be loaded with portions of the same sample. In some embodiments, a dissociation curve correction can be calibrated for the sample, prior to a run of an experiment using such sample. In some embodiments, a method is provided for generating a melting transition region of dissociation curves that show the melting characteristics of the sample. In some embodiments, dye temperature dependence correction can be performed on the dissociation curve data to further improve analysis. In some embodiments, a feature vector can be derived from the melt data, and the feature vector can be used to further improve genotyping analysis of the dissociation curves.

Abstract translation: 提供了对原始解离数据和解离曲线进行操作以产生检测数据的校准并进一步改进数据分析的方法。 可以从多区域平台的每个支撑区域（例如，从多孔板的每个孔）获取数据。 每个支撑区域可以加载相同样品的部分。 在一些实施方案中，在使用这种样品的实验运行之前，可以对样品校准解离曲线校正。 在一些实施方案中，提供了一种产生解析曲线的熔融转变区域的方法，其显示样品的熔融特性。 在一些实施方案中，可以对解离曲线数据进行染料温度依赖性校正，以进一步改进分析。 在一些实施例中，可以从熔体数据导出特征向量，并且可以使用特征向量来进一步改进解离曲线的基因分型分析。

公开(公告)号：US09772339B2

公开(公告)日：2017-09-26

申请号：US14348031

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Fortescue ,  Ming Shen ,  Woon Liang Soh ,  Francis T. Cheng

IPC: G06K7/00 ,  G01N35/00 ,  G06T7/00 ,  G01N35/02 ,  G01N15/00

CPC classification number: G01N35/00732 ,  G01N35/028 ,  G01N2015/0053 ,  G01N2035/00752 ,  G06T7/0012 ,  G06T2207/10004 ,  G06T2207/30004

Abstract: A computer-implemented method for identifying a first object-of-interest is provided. The first object-of-interest includes two identifiers and a sample portion. The method includes imaging the first object-of-interest including the two identifiers. The imaging generates a first set of image data. The method further includes determining a position of the first object-of-interest in the field-of-view of an optical sensor and determining the two identifiers from the first set of image data. The method includes identifying the first object-of-interest based on the two identifiers.

公开(公告)号：US20140294268A1

公开(公告)日：2014-10-02

申请号：US14348206

申请日：2012-09-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jacob K. Freudenthal ,  Jeffrey A. Marks ,  Francis T. Cheng ,  Thomas Wessel

IPC: G06T7/00

CPC classification number: G06T7/0012 ,  G06T5/008 ,  G06T5/50 ,  G06T2207/10024 ,  G06T2207/10064 ,  G06T2207/10144 ,  G06T2207/20021 ,  G06T2207/20216 ,  G06T2207/30072

Abstract: A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.

Abstract translation: 提供了一种用于校准生物仪器的方法。 该方法包括以下步骤：获取至少一个生物样本阵列的图像，确定图像内的第一感兴趣区域，其中所述第一感兴趣区域包括所述至少一个生物阵列上的第一多个位置; 以及在所述第一感兴趣区域内识别与所述至少一个生物阵列上的所述第一多个位置中的每一个相关联的多个图像元素。