公开(公告)号：US11208688B2

公开(公告)日：2021-12-28

申请号：US15887159

申请日：2018-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda Wong ,  Caifu Chen ,  Yalei Wu ,  Shoulian Dong ,  Chunmei Liu

IPC: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6832 ,  C12Q1/6848

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

公开(公告)号：US10954553B2

公开(公告)日：2021-03-23

申请号：US15208215

申请日：2016-07-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shoulian Dong ,  Chunmei Liu

IPC: C12P19/34 ,  C12Q1/6855 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6832 ,  C12Q1/6848

Abstract: The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.

公开(公告)号：US09416405B2

公开(公告)日：2016-08-16

申请号：US14071444

申请日：2013-11-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shoulian Dong ,  Chunmei Liu

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/191 ,  C12Q2525/301

Abstract: The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.

Abstract translation: 本公开提供了用于检测特异性核酸序列的新型引物和方法。 本文提供的引物和方法可用于多种分子生物学应用，并且特别适用于等位基因特异性PCR。