公开(公告)号：US09365838B2

公开(公告)日：2016-06-14

申请号：US14087307

申请日：2013-11-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Theo Nikiforov ,  Daniel Mazur ,  Xinzhan Peng ,  Tommie Lloyd Lincecum ,  Yuri Belosludtsev ,  Howard Reese ,  Dmitriy Gremyachinskiy ,  Roman Rozhkov ,  John Mauro ,  Joseph Beechem ,  Eric Tulsky ,  Imad Naasani ,  Kari Haley ,  Joseph Treadway

IPC: C12N9/12 ,  C07H19/20 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

公开(公告)号：US20250011760A1

公开(公告)日：2025-01-09

申请号：US18768211

申请日：2024-07-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Andersen ,  Sihong Chen ,  Daniel Mazur ,  Xinzhan Peng ,  Guobin Luo

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C40B20/04 ,  C40B40/06 ,  C40B50/06

Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

公开(公告)号：US11008612B2

公开(公告)日：2021-05-18

申请号：US16123140

申请日：2018-09-06

Applicant: Life Technologies Corporation

Inventor： Joseph Beechem ,  Theo Nikiforov ,  Vi-En Choong ,  Xinzhan Peng ,  Guobin Luo ,  Cheng-Yao Chen ,  Michael Previte

IPC: C12Q1/6869 ,  C07H19/20 ,  C12N9/12 ,  C12N9/96 ,  C12Q1/6818 ,  G01N33/58 ,  G01N21/64

Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

公开(公告)号：US10093972B2

公开(公告)日：2018-10-09

申请号：US15166372

申请日：2016-05-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Theo Nikiforov ,  Daniel Mazur ,  Xinzhan Peng ,  Tommie Lloyd Lincecum ,  Yuri Belosludtsev ,  Howard Reese ,  Dmitriy Gremyachinskiy ,  Roman Rozhkov ,  John Mauro ,  Joseph Beechem ,  Eric Tulsky ,  Imad Naasani ,  Kari Haley ,  Joseph Treadway

IPC: C07K14/00 ,  C12Q1/6869 ,  C07H19/20 ,  C12N9/12 ,  C12N9/96 ,  C12Q1/6818 ,  G01N33/58 ,  G01N21/64

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.