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公开(公告)号:US11015220B2
公开(公告)日:2021-05-25
申请号:US16123166
申请日:2018-09-06
Applicant: Life Technologies Corporation
Inventor: Theo Nikiforov , Daniel Mazur , Xinzhan Peng , Tommie Lloyd Lincecum , Yuri Belosludtsev , Howard Reese , Dmitriy Gremyachinskiy , Roman Rozhkov , John Mauro , Joseph Beechem , Eric Tulsky , Imad Naasani , Kari Haley , Joseph Treadway
IPC: C12Q1/6869 , C07H19/20 , C12N9/12 , C12N9/96 , C12Q1/6818 , G01N33/58 , G01N21/64
Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.
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公开(公告)号:US10093972B2
公开(公告)日:2018-10-09
申请号:US15166372
申请日:2016-05-27
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Theo Nikiforov , Daniel Mazur , Xinzhan Peng , Tommie Lloyd Lincecum , Yuri Belosludtsev , Howard Reese , Dmitriy Gremyachinskiy , Roman Rozhkov , John Mauro , Joseph Beechem , Eric Tulsky , Imad Naasani , Kari Haley , Joseph Treadway
IPC: C07K14/00 , C12Q1/6869 , C07H19/20 , C12N9/12 , C12N9/96 , C12Q1/6818 , G01N33/58 , G01N21/64
Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.
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公开(公告)号:US20180305673A1
公开(公告)日:2018-10-25
申请号:US15959839
申请日:2018-04-23
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Peter Vander Horn , Theo Nikiforov , Guobin Luo , Mindy Landes , Daniel Mazur , Eileen Tozer , Tommie Lloyd Lincecum
IPC: C12N9/12 , C12Q1/686 , C12Q1/6844
CPC classification number: C12N9/1252 , C12Q1/6846 , C12Q1/686 , C12Y207/07007
Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.
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公开(公告)号:US09365838B2
公开(公告)日:2016-06-14
申请号:US14087307
申请日:2013-11-22
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Theo Nikiforov , Daniel Mazur , Xinzhan Peng , Tommie Lloyd Lincecum , Yuri Belosludtsev , Howard Reese , Dmitriy Gremyachinskiy , Roman Rozhkov , John Mauro , Joseph Beechem , Eric Tulsky , Imad Naasani , Kari Haley , Joseph Treadway
CPC classification number: C12Q1/6869 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12N9/96 , C12Q1/6818 , C12Y207/07 , C12Y207/07007 , G01N21/6428 , G01N33/582 , G01N2021/6432
Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.
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