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公开(公告)号：US07570443B2
公开(公告)日：2009-08-04
申请号：US11086275
申请日：2005-03-22
申请人： Barry J. Blasenheim , H. Pin Kao , Mark F. Oldham
发明人： Barry J. Blasenheim , H. Pin Kao , Mark F. Oldham
IPC分类号： G02B7/02
CPC分类号： G01N21/253 , B01L3/0293 , B01L3/5025 , B01L3/5027 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2200/142 , B01L2300/021 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1883 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/0099 , G01N35/028 , G01N35/1002 , G01N35/1074 , G01N2035/00287 , G01N2035/00366 , G01N2035/0405 , G01N2035/0425 , G01N2035/1039
摘要： A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.
摘要翻译： 一种相机对准系统，其能够实现三个平面中的至少一个和至少一个平面的轴线的对准。 对准安装座可与摄像机和镜头配合使用。 对准安装座可以包括一个机构，用于相机相对于镜头调整到照相机的图像平面与预定取向的透镜的图像平面对准。 一个预定方向可以是相机的图像平面平行于透镜的图像平面。
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公开(公告)号：US08089623B2
公开(公告)日：2012-01-03
申请号：US12543459
申请日：2009-08-18
申请人： H. Pin Kao , Ian A. Harding , Achim Karger , Mark F. Oldham , Omead Ostadan , Greg Young
发明人： H. Pin Kao , Ian A. Harding , Achim Karger , Mark F. Oldham , Omead Ostadan , Greg Young
CPC分类号： G01N35/028 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N2035/00366 , H04N5/361 , H04N5/367 , Y10T436/10
摘要： Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.
摘要翻译： 用于使用设置在多个反应室中的至少一个中的参考标准或非荧光物质的仪器的输出正常化的方法。 该方法包括收集和分析与参考标准或非荧光物质相关联的信号以确定归一化偏差。 然后将归一化偏压施加到从多个反应室的其余部分收集的数据信号。
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公开(公告)号：US20100113285A1
公开(公告)日：2010-05-06
申请号：US12431685
申请日：2009-04-28
申请人： H. Pin Kao , Ian A. Harding , Kathleen C. Hayashibara , Achim Karger , Fiona C. Laird Hyland , Mark F. Oldham , Omead Ostadan , Mark R. Pratt , Junko F. Stevens , Austin B. Tomaney , Greg Young
发明人： H. Pin Kao , Ian A. Harding , Kathleen C. Hayashibara , Achim Karger , Fiona C. Laird Hyland , Mark F. Oldham , Omead Ostadan , Mark R. Pratt , Junko F. Stevens , Austin B. Tomaney , Greg Young
CPC分类号： G01N35/028 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/523 , B01L9/56 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , C12Q1/686 , C12Q2600/166 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N35/0099 , G01N2035/00356 , G01N2035/042 , G16H10/40 , H04N5/361 , H04N5/367 , C12Q2545/113
摘要： A method of using a standard to correct for variability in sample handling, can comprise (a) adding a template of known concentration to an assay comprising a sample; (b) preamplifying the assay; (c) amplifying the assay; (d) collecting data during the amplifying; and (e) correcting the data using a comparison of data collected from the template to data collected from the sample.
摘要翻译： 使用标准来校正样品处理的变异性的方法可以包括（a）将已知浓度的模板加入到包含样品的测定中; （b）预扩增分析; （c）扩增测定; （d）放大期间收集数据; 和（e）使用从模板收集的数据与从样本收集的数据的比较来校正数据。
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公开(公告)号：US20080288179A1
公开(公告)日：2008-11-20
申请号：US12176656
申请日：2008-07-21
申请人： H. Pin Kao , Ian A. Harding , Achim Karger , Mark F. Oldham , Omead Ostadan , Greg Young
发明人： H. Pin Kao , Ian A. Harding , Achim Karger , Mark F. Oldham , Omead Ostadan , Greg Young
IPC分类号： G06F19/00
CPC分类号： G01N35/028 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N2035/00366 , H04N5/361 , H04N5/367 , Y10T436/10
摘要： Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.
摘要翻译： 用于使用设置在多个反应室中的至少一个中的参考标准或非荧光物质的仪器的输出正常化的方法。 该方法包括收集和分析与参考标准或非荧光物质相关联的信号以确定归一化偏差。 然后将归一化偏压施加到从多个反应室的其余部分收集的数据信号。
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公开(公告)号：US07417726B2
公开(公告)日：2008-08-26
申请号：US11087103
申请日：2005-03-22
申请人： H. Pin Kao , Ian A. Harding , Achim Karger , Mark F. Oldham , Omead Ostadan , Greg Young
发明人： H. Pin Kao , Ian A. Harding , Achim Karger , Mark F. Oldham , Omead Ostadan , Greg Young
CPC分类号： G01N35/028 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N2035/00366 , H04N5/361 , H04N5/367 , Y10T436/10
摘要： An apparatus for normalizing a PCR instrument, can comprise a microplate comprising at least 6,000 wells and a system of dyes at known concentrations in a plurality of the at least 6,000 wells. A method for normalizing a system can comprise (a) providing a microplate comprising at least 6,000 wells and a system of dyes; (b) exciting at least one dye; (c) detecting an emission output for the at least one dye; (d) determining if the emission output is in an acceptable range; and (e) adjusting the system so that the emission output is in the acceptable range.
摘要翻译： 用于标准化PCR仪器的装置可以包括在多个至少6000个孔中包含至少6,000个孔的微板和已知浓度的染料体系。 标准化系统的方法可以包括（a）提供包含至少6,000个孔和染料体系的微板; （b）激发至少一种染料; （c）检测所述至少一种染料的排放输出; （d）确定排放量是否在可接受的范围内; 和（e）调整系统使发射输出在可接受的范围内。
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