公开(公告)号：US20070187588A1

公开(公告)日：2007-08-16

申请号：US11624248

申请日：2007-01-18

申请人： Kiyomi Yoshinari ,  Yasushi Terui ,  Toshiyuki Yokosuka ,  Kinya Kobayashi ,  Atsumu Hirabayashi

发明人： Kiyomi Yoshinari ,  Yasushi Terui ,  Toshiyuki Yokosuka ,  Kinya Kobayashi ,  Atsumu Hirabayashi

IPC分类号： H01J49/00

CPC分类号： H01J49/02 ,  H01J49/004

摘要： The present invention provides a tandem type mass analysis system capable of carrying out the differential analysis with high efficiency by the tandem type mass analysis. A predetermined number of m/z regions are set up for carrying out the mass analysis with the all ions included therein being dissociated collectively for each m/z region so as to obtain measurement MS2 data. By comparing the measurement MS2 data with reference MS2 data stored in a reference data base, a difference thereof is detected. For the m/z region with a differential component detected, the mass analysis is carried out collectively without dissociation for the all ions included therein so as to obtain measurement MS1 data. By comparing the measurement MS1 data with the reference MS1 data, a difference thereof is detected. From the difference thereof, a parent ion considered to be the differential component factor is presumed for carrying out the mass analysis with the same being dissociated.

摘要翻译： 本发明提供一种能够通过串联型质量分析以高效率进行差示分析的串联式质量分析系统。 设定预定数量的m / z区域，用于进行质量分析，其中包含的全部离子为每个m / z区域共同解离，以获得测量MS 2的数据。 通过将测量MS 2 SUP数据与存储在参考数据库中的参考MS 2 SUP数据进行比较，检测其差异。 对于检测到差分成分的m / z区域，对于包含在其中的全部离子而不解离地进行质量分析，以获得测量MS 1的数据。 通过将测量MS＆lt; 1＆gt;数据与参考MS＆lt; 1＆gt;数据进行比较，检测其差异。 从它们的差异来看，推测认为是差分成分因子的母离子用于进行相同解离的质量分析。

公开(公告)号：US20060248942A1

公开(公告)日：2006-11-09

申请号：US11319611

申请日：2005-12-29

申请人： Fujio Oonishi ,  Kenichi Shinbo ,  Ritsuro Orihashi ,  Yasushi Terui ,  Tsukasa Shishika

发明人： Fujio Oonishi ,  Kenichi Shinbo ,  Ritsuro Orihashi ,  Yasushi Terui ,  Tsukasa Shishika

IPC分类号： G01N19/10

CPC分类号： H01J49/0036 ,  H01J49/40

摘要： For the achievement of data transfer time reduction, removal of noise data, and analytical efficiency improvement in an ADC data processing function of a time-of-flight mass spectrometer, the mass spectrometer comprises a data acquisition circuit including: an A/D converter; a signal intensity addition memory that stores data of ion signals such as a time range and the number of measurements and performs an addition process; a voltage value frequency addition memory that performs an addition process of frequencies of voltage values of the predetermined time range and the number of measurements and stores addition results; a threshold level computation circuit that computes a predetermined threshold level from the results in the memory; a compression memory that extracts only data exceeding the threshold level from the data in the signal intensity addition memory; and a counter that controls a measurement time for data acquisition and the operation of each circuit.

摘要翻译： 为了实现数据传输时间减少，噪声数据的去除以及飞行时间质谱仪的ADC数据处理功能的分析效率改进，质谱仪包括数据采集电路，包括：A / D转换器; 信号强度相加存储器，其存储诸如时间范围和测量次数的离子信号的数据，并执行加法处理; 电压值频率相加存储器，其执行预定时间范围的电压值的频率和测量次数的加法处理，并存储加法结果; 阈值电平计算电路，用于根据存储器中的结果计算预定的阈值电平; 压缩存储器，其从信号强度相加存储器中的数据中仅提取超过阈值电平的数据; 以及控制数据采集和每个电路的操作的测量时间的计数器。

公开(公告)号：US07126113B2

公开(公告)日：2006-10-24

申请号：US11041864

申请日：2005-01-25

申请人： Toshiyuki Yokosuka ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari

发明人： Toshiyuki Yokosuka ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/0036 ,  H01J49/004

摘要： According to the existing mass spectrometric system, whether or not the informations are sufficient for analyzing substances (particularly proteins, sugars, etc.) cannot be judged in the process of measurement. Further, it is difficult to find out isomers having just the same mass number or compounds very close in mass only from the MS data. According to this invention, whether or not the retention time in the LC (or GC) of peptide formed at the time of enzymatic decomposition of protein coincides with the predicted retention time assumed from the amino acid sequence predicted from MS2 mass spectrometry data is judged within the actual time period of measurement, and thereby the quality of MS2 mass spectrometry data (quantity of information) is judged.

摘要翻译： 根据现有的质谱系统，不能在测量过程中判断信息是否足以分析物质（特别是蛋白质，糖等）。 此外，难以从MS数据中发现质量数量相同或质量非常接近的异构体。 根据本发明，在蛋白质酶促分解时形成的肽的LC（或GC）中的保留时间是否与从MS2预测的氨基酸序列假定的预测保留时间一致， SUP>质谱数据在实际测量时间段内进行判断，从而判断MS质谱质谱数据（信息量）的质量。

公开(公告)号：US07064319B2

公开(公告)日：2006-06-20

申请号：US10401944

申请日：2003-03-31

申请人： Yuichiro Hashimoto ,  Izumi Waki ,  Kiyomi Yoshinari ,  Yasushi Terui ,  Tsukasa Shishika ,  Marvin L. Vestal

发明人： Yuichiro Hashimoto ,  Izumi Waki ,  Kiyomi Yoshinari ,  Yasushi Terui ,  Tsukasa Shishika ,  Marvin L. Vestal

IPC分类号： H01J49/42

CPC分类号： H01J49/4205 ,  H01J49/004 ,  H01J49/0481 ,  H01J49/063 ,  H01J49/40

摘要： A mass spectrometer according to the present invention has an ionization source for generating ions; an ion trap for accumulating the ions; a time-of-flight mass spectrometer for performing mass spectrometry analysis on the ions by use of a flight time; a collision damping chamber disposed between the ion trap and the time-of-flight mass spectrometer and having a plurality of electrodes therein, which produce a multi-pole electric field, wherein a gas is introduced into the collision damping chamber to reduce kinetic energy of the ions ejected from the ion trap; and an ion transmission adjusting mechanism disposed between the ion trap and the collision damping chamber to allow or prevent injection of the ions from the ion trap to the collision damping chamber. The mass spectrometer provides greatly enhanced qualitative and quantitative analysis capabilities, as compared with conventional techniques.

摘要翻译： 根据本发明的质谱仪具有用于产生离子的电离源; 用于积聚离子的离子阱; 飞行时间质谱仪，用于通过使用飞行时间对离子进行质谱分析; 设置在离子阱和飞行时间质谱仪之间并且具有多个电极的碰撞阻尼室，其产生多极电场，其中气体被引入到碰撞阻尼室中以降低动能 从离子阱排出的离子; 以及设置在所述离子阱和所述碰撞阻尼室之间的离子透射调节机构，以允许或防止离子从所述离子阱向所述碰撞阻尼室注入。 与常规技术相比，质谱仪提供了大大提高的定性和定量分析能力。

公开(公告)号：US5822059A

公开(公告)日：1998-10-13

申请号：US633234

申请日：1996-04-16

申请人： Hayato Tobe ,  Kazuo Moriya ,  Yoshisada Ebata ,  Yasushi Terui

发明人： Hayato Tobe ,  Kazuo Moriya ,  Yoshisada Ebata ,  Yasushi Terui

IPC分类号： G01N21/31 ,  G01N21/74 ,  G01J3/30

CPC分类号： G01N21/74

摘要： The invention intends to provide an atomic absorption spectrophotometer which can establish a uniform heat distribution during heating of a sample and can improve analysis accuracy and analyzing efficiency. For this purpose, a graphite tube type cuvette mounted in a graphite atomizer furnace for an atomic absorption spectrophotometer comprises a large-diameter portion for retaining a sample in place, a small-diameter portion connected to the large-diameter portion and having a smaller diameter than the large-diameter portion, and a step portion for demarcating between the large-diameter portion and the small-diameter portion. The graphite tube type cuvette is formed such that its cross-sectional area in a plane perpendicular to the direction of passage of an electric current supplied to the cuvette is the same in any of the large-diameter portion, the small-diameter portion and the step portion. The amount of resistance heat is thereby also the same in any portions.

摘要翻译： 本发明旨在提供一种原子吸收分光光度计，可在样品加热期间建立均匀的热分布，并可提高分析精度和分析效率。 为此，安装在用于原子吸收分光光度计的石墨雾化炉中的石墨管型比色皿包括用于将样品保持在适当位置的大直径部分，与大直径部分连接并具有较小直径的小直径部分 和大直径部分之间划分的台阶部分，以及用于在大直径部分和小直径部分之间划定的台阶部分。 石墨管型比色皿形成为使得其在垂直于供给到比色杯的电流的通过方向的平面中的横截面面积在大直径部分，小直径部分和 步骤部分。 因此，电阻热的量在任何部分也是相同的。

公开(公告)号：US07890074B2

公开(公告)日：2011-02-15

申请号：US11675173

申请日：2007-02-15

申请人： Kenichi Shinbo ,  Fujio Oonishi ,  Ritsuro Orihashi ,  Yasushi Terui ,  Tsukasa Shishika

发明人： Kenichi Shinbo ,  Fujio Oonishi ,  Ritsuro Orihashi ,  Yasushi Terui ,  Tsukasa Shishika

IPC分类号： H04B17/00

CPC分类号： H01J49/0036

摘要： In a data acquisition system of ADC system, a log amplifier is provided at the pre-stage of an A/D converter, a signal amplified by the log amplifier having a nonlinear input-output characteristic is A/D-converted, and an adding operation of data is performed while reconverting a voltage value data which is converted to a nonlinear characteristic to data with a linear scale according to a table memory for reverse-log conversion. A known voltage value is inputted into the log amplifier to perform measurement, and calibration of the table memory is performed by storing the voltage value and the voltage value data after A/D-converted.

摘要翻译： 在ADC系统的数据采集系统中，在A / D转换器的前级提供对数放大器，由具有非线性输入输出特性的对数放大器放大的信号进行A / D转换， 执行数据的操作，同时根据用于反向对数转换的表存储器，将转换成非线性特性的电压值数据重新转换成具有线性刻度的数据。 已知的电压值被输入到对数放大器中以进行测量，并且通过在A / D转换后存储电压值和电压值数据来执行表存储器的校准。

公开(公告)号：US07595484B2

公开(公告)日：2009-09-29

申请号：US11315162

申请日：2005-12-23

申请人： Toshiyuki Yokosuka ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Atsushi Otake ,  Atsumu Hirabayashi ,  Yasushi Terui

发明人： Toshiyuki Yokosuka ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Atsushi Otake ,  Atsumu Hirabayashi ,  Yasushi Terui

IPC分类号： B01D59/44

CPC分类号： H01J49/004 ,  H01J49/0031

摘要： The present invention can provide a mass spectrometric system judging whether a measurement target is a substance required by an operator within an actual measurement time, when a substance (particularly such as protein or sugar chains) is analyzed. In the mass spectrometric system using a tandem mass spectrometer, a particular substance obtained by separating a sample is ionized, and mass analysis of the ionized substance is performed to obtain a spectrum. This spectrum is compared with a particular spectrum stored in advance, to thereby determine whether both the spectra match with each other. When a match is determined, a particular ion is further ionized within a particular time for detailed analysis. The invention also provides a mass spectrometric method, a diagnosis system and an inspection system each using the mass spectrometric system, and a program for operating a computer to control those systems with desired functions.

摘要翻译： 当分析物质（特别是蛋白质或糖链）时，本发明可以提供质谱系统，判定测量对象是否是实际测量时间内操作者所需的物质。 在使用串联质谱仪的质谱系统中，将通过分离样品获得的特定物质电离，并进行离子化物质的质量分析以获得光谱。 将该频谱与预先存储的特定谱进行比较，从而确定两个谱是否彼此匹配。 当确定匹配时，特定离子在特定时间内进一步电离以进行详细分析。 本发明还提供了使用质谱系统的质谱法，诊断系统和检查系统，以及用于操作计算机以控制具有期望功能的那些系统的程序。

公开(公告)号：US20090189063A1

公开(公告)日：2009-07-30

申请号：US12330374

申请日：2008-12-08

申请人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

发明人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

IPC分类号： H01J49/00

CPC分类号： G01N33/6851 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/004

摘要： During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.

摘要翻译： 在通过串联质谱法对蛋白质或肽进行结构分析期间，作为串联质谱目标，可以避免衍生自已经测量并且大量表达的蛋白质的肽离子。 迄今为止难以分析的，从微量蛋白质衍生的肽可以在实时测定中作为串联质谱图目标自动确定。 关于已经测量的蛋白质和源自蛋白质的肽的数据被自动存储在内部数据库中。 存储的数据与测量数据高精度对照以确定同位素峰。 以这种方式，可以在测量的实际时间内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。 包含在MSn谱中的信息在涉及多级解离和质谱（MSn）的MSn的每个步骤中被有效地利用，使得用于确定下一个分析内容的流程和为母体离子的选择 例如，MSn + 1分析可以在实时测量和高效率和精确度下进行优化。 因此，用户关注的目标可以进行串联质谱，无需浪费测量。

公开(公告)号：US20080073504A1

公开(公告)日：2008-03-27

申请号：US11675173

申请日：2007-02-15

申请人： Kenichi Shinbo ,  Fujio Oonishi ,  Ritsuro Orihashi ,  Yasushi Terui ,  Tsukasa Shishika

发明人： Kenichi Shinbo ,  Fujio Oonishi ,  Ritsuro Orihashi ,  Yasushi Terui ,  Tsukasa Shishika

IPC分类号： H01J49/00

CPC分类号： H01J49/0036

摘要： In a data acquisition system of ADC system, a log amplifier is provided at the pre-stage of an A/D converter, a signal amplified by the log amplifier having a nonlinear input-output characteristic is A/D-converted, and an adding operation of data is performed while reconverting a voltage value data which is converted to a nonlinear characteristic to data with a linear scale according to a table memory for reverse-log conversion. A known voltage value is inputted into the log amplifier to perform measurement, and calibration of the table memory is performed by storing the voltage value and the voltage value data after A/D-converted.

摘要翻译： 在ADC系统的数据采集系统中，在A / D转换器的前级提供对数放大器，由具有非线性输入输出特性的对数放大器放大的信号进行A / D转换， 执行数据的操作，同时根据用于反向对数转换的表存储器，将转换成非线性特性的电压值数据重新转换成具有线性刻度的数据。 已知的电压值被输入到对数放大器中以进行测量，并且通过在A / D转换后存储电压值和电压值数据来执行表存储器的校准。

公开(公告)号：US06765200B2

公开(公告)日：2004-07-20

申请号：US09792582

申请日：2001-02-23

申请人： Akira Maekawa ,  Akira Owada ,  Yasushi Terui

发明人： Akira Maekawa ,  Akira Owada ,  Yasushi Terui

IPC分类号： H01J4910

CPC分类号： H01J49/105

摘要： In order to provide an equipment that permits easy elimination of interference ions even in a low resolution mass spectrometer, the isotope mass number information and the isotope presence ratio information for each element are stored, and processing is done as is shown in FIG. 1 using such information and the measurement results, and the amount of target elemental ions is calculated by calculating and eliminating the amount of interference caused by other elemental isotopes to the target element. Because of this, when carrying out element analysis using a mass spectrometer with a relatively low resolution, the user can easily obtain the measurement result of the amount of any target elemental ions.

摘要翻译： 为了提供即使在低分辨率质谱仪中也容易消除干扰离子的设备，存储每个元件的同位素质量数信息和同位素存在比信息，并且如图1所示进行处理。 使用这样的信息和测量结果，并且通过计算和消除由其他元素同位素对目标元素引起的干扰量来计算目标元素离子的量。 因此，当使用分辨率相对较低的质谱仪进行元素分析时，用户可以容易地获得任何目标元素离子的量的测量结果。