公开(公告)号：US20230332069A1

公开(公告)日：2023-10-19

申请号：US18055373

申请日：2022-11-14

Applicant: Novozymes A/S

Inventor： Christian Hans Holm ,  Per Munk Nielsen ,  Fanny Longin ,  Sara Maria Landvik ,  Jesper Brask ,  Kim Borch ,  Robert Piotr Olinski ,  Allan Noergaard ,  Hanna Maria Lilbaek ,  Marianne Linde Damstrup ,  Tianqi Sun ,  Ming Li

IPC: C11B3/00 ,  C12N9/18

CPC classification number: C11B3/003 ,  C12N9/18

Abstract: The present invention relates to refining of vegetable oil. The invention provides processes in which phospholipids present in the vegetable oil are hydrolysed and the oil is subsequently subject to chemical refining.

公开(公告)号：US20180355404A1

公开(公告)日：2018-12-13

申请号：US15563692

申请日：2016-04-07

Applicant: NOVOZYMES A/S

Inventor： Robert Blazej ,  Nicholas Toriello ,  Charles Emrich ,  Allan Svendsen ,  Jesper Brask ,  Anne Olsen ,  Kathleen Hirano ,  Amy Twite

IPC: C12Q1/44

CPC classification number: C12Q1/44 ,  C12Q1/34

Abstract: Provided herein are methods and means for enhancing lipase activity. The system makes use of an emulsion for in vitro compartmentalization of a library of synthetic compounds which have a polynucleotide linked to a lipase substrate (e.g., a triglyceride). Expressed polypeptides having greater lipase activity will preferentially hydrolyze the substrate from the linked polynucleotide. Genes encoding polypeptides having less lipase activity will remain linked to the substrate and may be removed to enrich the library for more active variants. Also described are synthetic compounds and emulsions which can be used in the methods.

公开(公告)号：US09670470B2

公开(公告)日：2017-06-06

申请号：US14777617

申请日：2014-03-21

Applicant: Novozymes A/S

Inventor： Kim Borch ,  Sara Landvik ,  Marianne Linde Damstrup ,  Jesper Brask

IPC: C12N9/12 ,  C12P7/64 ,  C12N9/20 ,  C12N9/18 ,  C11B3/00

CPC classification number: C12N9/20 ,  C11B3/003 ,  C12N9/18 ,  C12Y301/01

Abstract: The present invention relates to isolated polypeptides having phospholipase A activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20170096620A1

公开(公告)日：2017-04-06

申请号：US15128010

申请日：2015-03-27

Applicant: Novozymes A/S

Inventor： Tianqi Sun ,  Ming Li ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Marianne Linde Damstrup ,  Hanna Maria Lilbaek

IPC: C11B3/00 ,  C12N15/52 ,  C12N9/20

CPC classification number: C11B3/003 ,  C07H21/00 ,  C12N9/20 ,  C12N15/52 ,  C12Y301/04003

Abstract: The present invention provides polypeptides having phospholipase C activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides. Furthermore, the present invention provides a method of reducing the phospholipid content in an oil composition using the polypeptide having phospholipase C activity as well as other relevant uses of the polypeptide.

公开(公告)号：US20170096619A1

公开(公告)日：2017-04-06

申请号：US15126098

申请日：2015-03-19

Applicant: Novozymes A/S

Inventor： Dorotea Raventos Segura ,  Robert Piotr Olinski ,  Kim Borch ,  Allan Noergaard ,  Jesper Brask ,  Rune Falkenberg Haller ,  Marianne Linde Damstrup

IPC: C11B3/00 ,  C12N9/20

CPC classification number: C11B3/003 ,  C12N9/16 ,  C12N9/20 ,  C12P7/6445 ,  C12Y301/04003 ,  C12Y301/04011

Abstract: The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20170058235A1

公开(公告)日：2017-03-02

申请号：US15308495

申请日：2015-05-15

Applicant: Novozymes A/S

Inventor： Marianne Linde Damstrup ,  Jesper Brask ,  Hanna Maria Lilbaek

IPC: C11B3/00 ,  C12N9/88 ,  C12N9/16

CPC classification number: C11B3/003 ,  A23K20/189 ,  C12N9/16 ,  C12N9/88 ,  C12P7/6418 ,  C12P7/649 ,  C12Y301/04003 ,  C12Y301/04011 ,  C12Y406/01013 ,  Y02E50/13

Abstract: The present invention relates to compositions comprising polypeptides having phospholipase C activity and to the use of such compositions for hydrolysing a phospholipid or lysophospholipid, The invention also relates to processes for hydrolysing a phospholipid or lysophospholipid, comprising contacting said phospholipid or lysophospholipid with a composition or a pluralities of polypeptides having Phospholipase C activity according to the invention.

Abstract translation: 本发明涉及包含具有磷脂酶C活性的多肽的组合物以及这种用于水解磷脂或溶血磷脂的组合物的用途。本发明还涉及水解磷脂或溶血磷脂的方法，包括使所述磷脂或溶血磷脂与组合物或 多种具有根据本发明的磷脂酶C活性的多肽。

公开(公告)号：US20160143309A1

公开(公告)日：2016-05-26

申请号：US15011920

申请日：2016-02-01

Applicant: Novozymes A/S

Inventor： Lan Tang ,  Ye Liu ,  Kim Borch ,  Jesper Brask

IPC: A23D9/04 ,  C12N9/16

CPC classification number: A23D9/04 ,  C11B3/003 ,  C12N9/16 ,  C12N9/20 ,  C12Y301/04003

Abstract: The present invention relates to isolated polypeptides having phospholipase C activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 本发明涉及具有磷脂酶C活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。