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    Yield Improvement by PH-Stabilization of Enzymes
    11.
    发明申请
    Yield Improvement by PH-Stabilization of Enzymes 审中-公开
    通过酶的稳定化产生的产量提高

    公开(公告)号：US20160319264A1

    公开(公告)日：2016-11-03

    申请号：US15108344

    申请日：2015-01-08

    Applicant: NOVOZYMES A/S

    Inventor： Tomoko Matsui

    IPC: C12N9/44 ,  C12Q1/40 ,  C12N9/96

    CPC classification number: C12N9/2457 ,  C12N9/00 ,  C12N9/96 ,  C12Q1/40 ,  C12Y302/01041 ,  G01N2333/944

    Abstract: The invention relates to methods of improving the yield or productivity of a variant enzyme derived from a parent enzyme, said method comprising the step of selecting a host cell that produces a variant enzyme which has at least the specific enzymatic activity of the parent enzyme as well as an improved pH-stability in the pH range of 5-9.

    Abstract translation: 本发明涉及提高衍生自亲本酶的变体酶的产量或生产率的方法，所述方法包括选择产生至少具有亲本酶的特异性酶活性的变体酶的宿主细胞的步骤 作为在5-9的pH范围内改善的pH稳定性。

    Serine protease variants and polynucleotides encoding same
    13.
    发明授权
    Serine protease variants and polynucleotides encoding same 有权

    公开(公告)号：US12291730B2

    公开(公告)日：2025-05-06

    申请号：US17547098

    申请日：2021-12-09

    Applicant: Novozymes A/S

    Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben Peter Friis ,  Jens Erik Nielsen ,  Roland Alexander Pache

    IPC: C12N9/58 ,  C12P7/06

    Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

    Polypeptides having pullulanase activity suitable for use in liquefaction
    16.
    发明授权
    Polypeptides having pullulanase activity suitable for use in liquefaction 有权

    公开(公告)号：US11427811B2

    公开(公告)日：2022-08-30

    申请号：US15746162

    申请日：2016-07-11

    Applicant: NOVOZYMES A/S

    Inventor： Tomoko Matsui ,  Suzanne Clark ,  Aki Tomiki

    IPC: C12N9/44 ,  C12P7/06 ,  C12P19/02 ,  C12P19/14 ,  C12C5/00 ,  C12C7/04 ,  C12N9/34 ,  C13K1/06

    Abstract: The present invention relates to a variant pullulanase, wherein the pullulanase comprises at least the following combination of substitutions: N368G+N393A+Q431E+L432F+A492A,S+N610R+G624S+T631S+S632C, and optionally further comprises N222P+Q252A+Q256R; wherein the variant has pullulanase activity, and wherein the variants have at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3. Further aspect the present invention relates to a process for liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase and a thermo-stable pullulanase of the invention.

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