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    Long Non-Coding RNA-Expression In Fungal Hosts
    11.
    发明公开
    Long Non-Coding RNA-Expression In Fungal Hosts 审中-公开

    公开(公告)号：US20230272353A1

    公开(公告)日：2023-08-31

    申请号：US16971174

    申请日：2019-02-21

    Applicant: Novozymes A/S

    Inventor： Debbie Yaver ,  Astrid Mach-Aigner ,  Robert Mach ,  Petra Till

    IPC: C12N9/02 ,  C12N15/113 ,  C12N15/80

    CPC classification number: C12N9/0061 ,  C12N15/1137 ,  C12Y302/01004 ,  C12N15/80 ,  C12N2800/10

    Abstract: The instant invention relates to a filamentous fungal host cell producing a polypeptide of interest, said host cell comprising one or more native or heterologous polynucleotide encoding a long non-coding RNA (lncRNA), wherein said lncRNA comprises more than 262 nucleotides and comprises a contiguous nucleotide sequence at its 3′ end, absent any poly-adenylation tail, that is at least 70% identical to that of SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 19, SEQ ID NO:20 or SEQ ID NO:21; and/or said lncRNA comprises 2 or more Xyr1 binding sequences, as well as methods of producing a polypeptide of interest in said host cells and methods of improving the production of a polypeptide of interest.

    Promoter Variants For Expressing Genes In A Fungal Cell
    17.
    发明申请
    Promoter Variants For Expressing Genes In A Fungal Cell 审中-公开

    公开(公告)号：US20170233751A1

    公开(公告)日：2017-08-17

    申请号：US15498011

    申请日：2017-04-26

    Applicant: Novozymes, Inc.

    Inventor： Debbie Yaver ,  Peter Nham

    IPC: C12N15/80 ,  C12N9/20 ,  C12N9/58 ,  C12N9/06 ,  C12N9/00

    CPC classification number: C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/58 ,  C12N9/93 ,  C12N15/113 ,  C12P1/02 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y301/01003 ,  C12Y304/21004 ,  C12Y603/04002

    Abstract: The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

    Methods for improving malic acid production in filamentous fungi
    19.
    发明申请
    Methods for improving malic acid production in filamentous fungi 审中-公开
    改善丝状真菌中苹果酸生产的方法

    公开(公告)号：US20150024446A1

    公开(公告)日：2015-01-22

    申请号：US14510582

    申请日：2014-10-09

    Applicant: Novozymes, Inc.

    Inventor： Stephen Brown ,  Sheryl Luttringer ,  Debbie Yaver ,  Alan Berry

    IPC: C12P7/46 ,  C12N15/80

    CPC classification number: C12P7/46 ,  C07K14/38 ,  C12N15/80

    Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.

    Abstract translation: 本发明涉及生产C4二羧酸的方法，其包括：（a）培养丝状真菌宿主细胞，所述丝状真菌宿主细胞包含选自以下的多核苷酸：编码C4二羧酸转运蛋白的异源第一多核苷酸，编码 苹果酸脱氢酶和编码丙酮酸羧化酶的异源第三多核苷酸; 其中当在相同条件下培养时，丝状真菌宿主细胞能够分泌与不含异源多核苷酸的丝状真菌宿主细胞相比增加的C4二羧酸水平; 和（b）回收C4二羧酸。 本发明还涉及增加C4二羧酸生产，丝状真菌宿主细胞和苹果酸脱氢酶变体的方法。

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