公开(公告)号：US20190093070A1

公开(公告)日：2019-03-28

申请号：US15998571

申请日：2017-01-31

Applicant: OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI

IPC: C12N5/00 ,  A61L27/50 ,  A61L27/22 ,  A61L27/38 ,  C12N5/077 ,  C12N5/071

Abstract: Provided is a method for producing a three-dimensional tissue having a vascular system structure, said method comprising: (a) a step for forming a vascular system structure template using a gel; (b) a step for forming a three-dimensional tissue in the vicinity of the template; (c) a step for dissolving the template using a cationic solution; and (d) a step for seeding vascular endothelial cells and/or lymphatic vessel endothelial cells in a void remaining after the dissolution of the template. Also provided is a method for producing a three-dimensional tissue having a vascular system structure, said method comprising: (i) a step for forming a vascular system structure template using a gel; (ii) a step for seeding vascular endothelial cells and/or lymphatic vessel endothelial cells on the template; (iii) a step for forming a three-dimensional tissue in the vicinity of the cells seeded above; and (iv) a step for dissolving the template using a cationic solution. Also provided is a three-dimensional tissue comprising a gel which has a vascular system structure.

公开(公告)号：US20170283778A1

公开(公告)日：2017-10-05

申请号：US15507083

申请日：2015-08-25

Applicant: HIROSAKI UNIVERSITY ,  OSAKA UNIVERSITY

Inventor： Hiroshi SHIMODA ,  Yoshiya ASANO ,  Mitsuru AKASHI ,  Michiya MATSUSAKI

IPC: C12N5/071

CPC classification number: C12N5/0697 ,  C12N11/02 ,  C12N2502/1192 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2513/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

Abstract: The present disclosure relates to: an artificial peritoneal tissue comprising a cellular tissue and a mesothelial cell layer that covers the surface of the cellular tissue, wherein the cellular tissue comprises a fibroblast, an extracellular matrix, and a vascular endothelial cell and/or a lymphatic endothelial cell each capable of forming a lumen; and a method for producing the artificial peritoneal tissue.

公开(公告)号：US20240301347A1

公开(公告)日：2024-09-12

申请号：US18578247

申请日：2022-06-29

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Asuka YAMADA ,  Shiro KITANO ,  Michiya MATSUSAKI

IPC: C12N5/00

CPC classification number: C12N5/0068 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2537/00

Abstract: One aspect of the present invention relates to a production method of a fragmented extracellular matrix component, the method including: a step of neutralizing a solution in which an extracellular matrix component is dissolved; a step of obtaining a mass containing the extracellular matrix component by removing the solvent through freeze-drying treatment after the neutralization step; a step of obtaining a liquid containing the fragmented extracellular matrix component by dispersing the mass in a solution containing a polar organic solvent and fragmenting the extracellular matrix component in the solution; and a step of removing a liquid component from the liquid containing the fragmented extracellular matrix component.

公开(公告)号：US20240191202A1

公开(公告)日：2024-06-13

申请号：US18552007

申请日：2022-03-02

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Yasuyuki HIRAOKA ,  Michiya MATSUSAKI

IPC: C12N5/071

CPC classification number: C12N5/0671 ,  C12N2501/91 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/56

Abstract: Disclosed is a method for producing a cell aggregate having bile canaliculi, including a contact step of bringing cells including hepatocytes into contact with heparin in an aqueous medium and a culture step of culturing the cells brought in contact with the heparin to form the cell aggregate.

公开(公告)号：US20240117313A1

公开(公告)日：2024-04-11

申请号：US18276912

申请日：2022-02-15

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY ,  Kyoto Prefectural Public University Corporation

Inventor： Shiro KITANO ,  Michiya MATSUSAKI ,  Fiona LOUIS ,  Yoshihiro SOWA

IPC: C12N5/077

CPC classification number: C12N5/0653 ,  C12N2500/36 ,  C12N2501/998 ,  C12N2506/1384 ,  C12N2513/00

Abstract: A method for producing a three-dimensional tissue construct comprising mature adipocytes, comprising incubating cells comprising at least fat-derived stein cells in the presence of one or more fatty acids selected from the group consisting of erucic acid, elaidic acid, oleic acid, palmitoleic acid, myristoleic acid, phytanic acid, and pristanic acid.

公开(公告)号：US20230389540A1

公开(公告)日：2023-12-07

申请号：US18250814

申请日：2021-10-15

Applicant: OSAKA UNIVERSITY ,  TOPPAN INC. ,  Kyoto Prefectural Public University Corporation

Inventor： Michiya MATSUSAKI ,  Fiona LOUIS ,  Shiro KITANO ,  Yoshihiro SOWA

IPC: A01N1/02

CPC classification number: A01N1/0284

Abstract: The present invention relates to a method for freezing a cell structure including freezing a cell structure including fragmented extracellular matrix components, cells and fibrin and having a three-dimensional tissue structure.

公开(公告)号：US20230323310A1

公开(公告)日：2023-10-12

申请号：US18043507

申请日：2021-09-03

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Tomoko KUNITOMI ,  Shiro KITANO ,  Kei TSUKAMOTO ,  Michiya MATSUSAKI

IPC: C12N5/071 ,  C12N5/077

CPC classification number: C12N5/069 ,  C12N5/0656 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2501/91

Abstract: The present invention relates to a method for producing a three-dimensional tissue body, including: a step of obtaining a mixture by mixing a cationic substance and a fragmented extracellular matrix component with cells; and a step of culturing the cells after the step of obtaining the mixture.

公开(公告)号：US20220130280A1

公开(公告)日：2022-04-28

申请号：US17441602

申请日：2020-03-25

Applicant: OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Agathe Elisabeth Marie FIGAROL ,  Kaoru SATO

IPC: G09B23/30 ,  C12N5/079

Abstract: Disclosed is a brain blood vessel model composed of a three-dimensional tissue containing defibrated extracellular matrix components and cells including brain microvascular endothelial cells, pericytes, and astrocytes, wherein at least a portion of the above-described cells adheres to the above-described defibrated extracellular matrix.

公开(公告)号：US20210063374A1

公开(公告)日：2021-03-04

申请号：US16644059

申请日：2018-09-03

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Shiro KITANO ,  Shinji IRIE

IPC: G01N33/15 ,  C12N5/077 ,  C07K14/78

Abstract: The present invention relates to an abnormal cardiac rhythm myocardial model composed of a three-dimensional tissue containing cells including the cardiomyocytes and collagen, wherein at least a portion of the cells adheres to the collagen.

公开(公告)号：US20250002865A1

公开(公告)日：2025-01-02

申请号：US18704322

申请日：2022-10-27

Applicant: TOPPAN Holdings Inc. ,  OSAKA UNIVERSITY ,  KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION

Inventor： Shiro KITANO ,  Michiya MATSUSAKI ,  Fiona LOUIS ,  Yoshihiro SOWA

IPC: C12N5/071

Abstract: There is provided a method for producing a tissue construct comprising vascular endothelial cells, the method comprising incubating cells comprising at least adipose-derived stem cells from human in the presence of a TGFβ type I receptor inhibitor.