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    Apparatus for detecting contamination in food products
    15.
    发明授权
    Apparatus for detecting contamination in food products 失效
    用于检测食品污染的装置

    公开(公告)号：US06180335B2

    公开(公告)日：2001-01-30

    申请号：US09410341

    申请日：1999-09-30

    申请人： Ebtisam S. Wilkins ,  Plamen B Atanassov ,  Dmitri M. Ivnitski ,  Ihab A. Abdel-Hamid

    发明人： Ebtisam S. Wilkins ,  Plamen B Atanassov ,  Dmitri M. Ivnitski ,  Ihab A. Abdel-Hamid

    IPC分类号： C12Q100

    CPC分类号： A01K43/00 ,  G01N33/5306 ,  G01N33/54393 ,  G01N33/56916 ,  G01N2333/245 ,  Y10S435/817

    摘要： The present invention is a disposable element for assaying food samples and a method for using the element. The disposable element includes a container having first, second, and third ports, a prefilter disposed in the container, an immuno-sorbent layer having antibodies to a target microbe affixed thereto, the immuno-sorbent layer also being disposed in the container, and an electrode in contact with the immuno-sorbent layer. The prefilter and immuno-sorbent layers are positioned in the container such that a sample introduced into the first port passes through the prefilter and the immuno-sorbent layer when a pressure differential is created between the first and third ports. In addition, liquid entering the second port passes through the immuno-sorbent layer, but not the prefilter, when a pressure differential is created between the second and third ports. The prefilter preferably has a pore size between 10 and 30 microns. The disposable element may be used to measure the level of microbiological contamination in a solid sample caused by a predetermined microbe. The sample is pretreated by mixing the sample with a buffer solution and blending buffer and sample. An aliquot of the blended sample is introduced into the disposable element and is filtered through the prefilter and then passes through the immuno-sorbent layer. A solution having labeled antibodies to the microbe is passed through the immuno-sorbent layer via the second port and the amount of label bound to the immuno-sorbent is measured.

    摘要翻译： 本发明是用于测定食物样品的一次性元件和使用该元件的方法。 一次性元件包括具有第一，第二和第三端口的容器，设置在容器中的预过滤器，具有附着于其上的靶微生物的抗体的免疫吸附层，还设置在容器中的免疫吸附剂层， 电极与免疫吸附剂层接触。 预过滤器和免疫吸附剂层定位在容器中，使得当在第一和第三端口之间产生压差时，引入第一端口的样品通过预过滤器和免疫吸附剂层。 此外，当在第二和第三端口之间产生压力差时，进入第二端口的液体通过免疫吸附剂层，而不是预过滤器。 预过滤器优选具有10至30微米的孔径。 一次性元件可用于测量由预定微生物引起的固体样品中微生物污染的水平。 样品通过将样品与缓冲溶液和混合缓冲液和样品混合来预处理。 将混合样品的等分试样引入一次性元件中，并通过预过滤器过滤，然后通过免疫吸附剂层。 将具有针对微生物的标记抗体的溶液经由第二端口通过免疫吸附层，并测量与免疫吸附剂结合的标记物的量。