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    Laser capture microdissection method and apparatus
    11.
    发明申请
    Laser capture microdissection method and apparatus 审中-公开
    激光捕获显微解剖方法和装置

    公开(公告)号：US20060176470A1

    公开(公告)日：2006-08-10

    申请号：US11299266

    申请日：2005-12-09

    申请人： Thomas Baer ,  Mark Enright ,  David Head ,  Christopher Todd

    发明人： Thomas Baer ,  Mark Enright ,  David Head ,  Christopher Todd

    IPC分类号： G01N1/00

    CPC分类号： G01N1/2813 ,  G01N1/2806 ,  G01N1/286 ,  G01N2001/2833 ,  G01N2001/284 ,  G02B21/32

    摘要： Systems and methods for laser capture microdissection are disclosed. One method incorporates the step of changing the beam diameter of the laser to adjust the size of the activated area of the transfer film and as a result, the size of the at least one microdissected portion of the sample adhered to the transfer film. Changing the beam diameter while operating the laser in idle mode permits the user to visualize the spot on the desired portion of the tissue sample and simultaneously increase or decrease the beam spot to optimally match the beam spot size with the portion of the tissue to be extracted. This method advantageously provides increased accuracy in microdissection and reduced heating of the tissue sample.

    摘要翻译： 公开了用于激光捕获显微切割的系统和方法。 一种方法包括改变激光束的光束直径以调节转印膜的活化区域的尺寸的步骤，结果，样品的至少一个显微切割部分的尺寸粘附到转印膜上。 在空闲模式下操作激光器时改变光束直径允许用户将组织样本的所需部分上的斑点可视化，并且同时增加或减少光束斑点，以将光束斑点尺寸与要提取的组织的部分最佳地匹配 。 该方法有利地提高了组织样本的显微切割和减少加热的增加的精度。

    3' Biased Detection of Nucleic Acids
    16.
    发明申请
    3' Biased Detection of Nucleic Acids 审中-公开
    3'核酸的偏置检测

    公开(公告)号：US20090270271A1

    公开(公告)日：2009-10-29

    申请号：US12210807

    申请日：2008-09-15

    申请人： Mark G. Erlander ,  Xiao-Jun Ma ,  Thomas Baer

    发明人： Mark G. Erlander ,  Xiao-Jun Ma ,  Thomas Baer

    IPC分类号： C40B30/04

    CPC分类号： C12Q1/6876 ,  C12Q1/6809 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/501

    摘要： The invention provides materials and methods for the detection of nucleic acid expression via the 3′ portion of expressed sequences. Embodiments of the invention include the use of microarrays comprising nucleic acid probes that are complementary to the 3′ end of expressed sequences and by the use of quantitative PCR (Q-PCR) based amplification of sequences found at or near the 3′ end of expressed sequences. The invention may be used to detect the presence of expressed nucleic acids encoding particular gene products (sequences present in a “transcriptome”).

    摘要翻译： 本发明提供了通过表达序列的3'部分检测核酸表达的材料和方法。 本发明的实施方案包括使用包含与表达的序列的3'末端互补的核酸探针的微阵列，以及通过使用基于定量PCR（Q-PCR）的扩增在3'末端发现的序列的表达 序列。 本发明可用于检测编码特定基因产物（“转录组”中存在的序列）的表达的核酸的存在。