公开(公告)号：US08460893B2

公开(公告)日：2013-06-11

申请号：US12279271

申请日：2007-02-15

申请人： Keiji Endo ,  Shenghao Liu ,  Katsutoshi Ara

发明人： Keiji Endo ,  Shenghao Liu ,  Katsutoshi Ara

IPC分类号： C12Q1/34 ,  C12N1/21 ,  C12P21/00 ,  C12N15/00 ,  C12N9/42

CPC分类号： C12N9/2437

摘要： The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress a Bacillus subtilis secY gene or a gene corresponding thereto.

摘要翻译： 本发明的目的是提供一种提高纤维素酶生产率并可用于纤维素酶的工业生产的微生物，并提供使用微生物生产纤维素酶的方法。 本发明提供了一种通过将编码纤维素酶的基因转移到已被遗传修饰以使其过表达枯草芽孢杆菌SecY基因或相应基因的亲本微生物而产生的重组微生物。

公开(公告)号：US20100323896A1

公开(公告)日：2010-12-23

申请号：US12868032

申请日：2010-08-25

申请人： Koichi Araki ,  Yoshitaka Sato ,  Takuya Gomibuchi ,  Keiji Endo ,  Shinichi Shirakura ,  Shin Nakamura ,  Christopher Rosinger ,  Dieter Feucht

发明人： Koichi Araki ,  Yoshitaka Sato ,  Takuya Gomibuchi ,  Keiji Endo ,  Shinichi Shirakura ,  Shin Nakamura ,  Christopher Rosinger ,  Dieter Feucht

IPC分类号： A01N43/66 ,  C07D239/52 ,  C07D251/16 ,  A01N43/54 ,  A01P13/00

CPC分类号： C07D251/20 ,  A01N43/54 ,  A01N43/66 ,  C07D239/52

摘要： Herbicidal compositions comprising containing sulfonanilides of the formula (I) wherein R1 represents hydrogen, fluorine, chlorine, C1-4 alkyl, C1-4 alkoxy, C3-6 cycloalkyl-C1-4 alkyloxy or C1-4 haloalkoxy, R2 represents hydrogen, fluorine or chlorine, R3 represents hydrogen or fluorine, R4 represents hydrogen or C1-4 alkyl which may be optionally C1-4 alkoxy-substituted, C3-6 alkenyl or C3-6 alkynyl, R5 represents hydrogen, R6 represents hydroxy, fluorine or chlorine, or R5 and R6 may form, together with the carbon to which they are bonded, C═O, and X represents CH or N, provided that the following cases are excluded: (i) R1 represents hydrogen, fluorine or chlorine, R2 represents hydrogen, R3 represents hydrogen, R4 represents hydrogen, R5 represents hydrogen, and R6 represents hydroxy, (ii) R1 represents hydrogen, fluorine or chlorine, R2 represents hydrogen, R3 represents hydrogen, R4 represents hydrogen, and R5 and R6 form C═O together with the carbon to which they are bonded, (iii) R1 represents C1-4 alkyl, R2 represents hydrogen, R3 represents hydrogen, R4 represents hydrogen, R5 represents hydrogen, R6 represents hydroxy, and X represents CH, or (iv) R1 represents C1-4 alkyl, R2 represents hydrogen, R3 represents hydrogen, R4 represents hydrogen, R5 and R6 form C═O together with the carbon to which they are bonded, and X represents CH, and new compounds being embraced by the formula (I).

摘要翻译： 包含含有式（I）的磺酰苯胺的除草组合物，其中R 1表示氢，氟，氯，C 1-4烷基，C 1-4烷氧基，C 3-6环烷基-C 1-4烷氧基或C 1-4卤代烷氧基，R 2表示氢，氟 或氯，R 3表示氢或氟，R 4表示氢或可以任选被C 1-4烷氧基取代的C 1-4烷基，C 3-6烯基或C 3-6炔基，R 5表示氢，R 6表示羟基，氟或氯， 或者R 5和R 6可以与它们所键合的碳一起形成C = O，X表示CH或N，条件是排除以下情况：（i）R 1表示氢，氟或氯，R 2表示氢 ，R3表示氢，R4表示氢，R5表示氢，R6表示羟基，（ii）R1表示氢，氟或氯，R2表示氢，R3表示氢，R4表示氢，R5和R6表示C = O 与它们结合的碳， （iii）R 1表示C 1-4烷基，R 2表示氢，R 3表示氢，R 4表示氢，R 5表示氢，R 6表示羟基，X表示CH，或（ⅳ）R 1表示C 1-4烷基， R3表示氢，R4表示氢，R5和R6与它们所键合的碳一起形成C = O，X表示CH，并且新化合物被式（I）所包围。

公开(公告)号：US07855165B2

公开(公告)日：2010-12-21

申请号：US11622514

申请日：2007-01-12

申请人： Keiji Endo ,  Shinichi Shirakura ,  Shin Nakamura ,  Natsuko Minegishi

发明人： Keiji Endo ,  Shinichi Shirakura ,  Shin Nakamura ,  Natsuko Minegishi

IPC分类号： A01N43/64 ,  A01N43/60

CPC分类号： A01N43/54 ,  A01N43/66 ,  A01N25/32 ,  A01N37/18 ,  A01N37/22 ,  A01N39/02 ,  A01N43/08 ,  A01N43/12 ,  A01N43/56 ,  A01N43/78 ,  A01N43/80 ,  A01N43/86 ,  A01N43/90 ,  A01N47/30 ,  A01N47/36 ,  A01N47/38 ,  A01N57/14 ,  A01N2300/00

摘要： A herbicide composition for paddy field characterized by containing (a) a herbicidal difluoromethanesulfonamide derivative represented by the formula (I) and (b) at least one herbicidal compound selected from the group consisting of pretilachlor, butachlor, alachlor, metolachlor, acetochlor, clomeprop, bromobutide, benfuresate, indanofan, pyrazolate, benzofenap, pyrazoxyfen, pyraclonil, oxaziclomefone, bensulfuron-methyl, azimsulfuron, imazosulfuron, pyrazosulfuron-ethyl, cyclosulfamuron, ethoxysulfuron, halosulfuron-methyl, orthosulfamuron, cinosulfuron, metsulfuron-methyl, penoxsulam, thiobencarb, pyributycarb, molinate, dimethametryn, simetryn, cafenstrole, quinclorac, anilofos, mefenacet, fentrazamide, pentoxazone, oxadiargyl, oxadiazon, benzobicyclon, mesotrione, AVH301 (code no.), cyhalofop-butyl, metamifop, bispyribac-sodium, pyriftalid, pyrimisulfan, pyrimenobac-methyl, chlormethoxynil, oxyfluorfen, dithiopyr, MCPA, MCPB, 2,4-D, dymron, cumyluron, quinoclamine and clomazone, and/or (c) one or more compounds selected from the group consisting of safeners dymron, isoxadifen(-ethyl), flurazole, fenchlorazole-ethyl, fenclorim, cloquintocet-mexyl, oxabetrinil, fluxofenim, mefenpyr-diethyl, furilazole, R-29148 (code no.), benoxacor, dichlormid and dicyclonone as effective components.

摘要翻译： 一种用于稻田的除草剂组合物，其特征在于含有（a）由式（I）和（b）表示的除草二氟甲磺酰胺衍生物，至少一种选自草酰草胺，丁草胺，甲草胺，异丙甲草胺，乙草胺，氯米普， 溴替丁，苯呋酮，吡唑啉，吡嘧磺隆，苯唑芬酮，吡嘧磺隆，吡嘧磺隆，恶唑磺隆，吡嘧磺隆，吡嘧磺隆，咪唑磺隆，吡嘧磺隆，环磺胺嘧啶，乙氧基磺隆，卤代磺隆，邻氨基磺隆， 茉莉酸，二甲基苯甲酸，西妥昔单抗，喹诺酮，芬太拉唑，依诺沙星，恶草精，恶二酮，苯并环己酮，甲基磺草酮，AVH301（编号），cyhalofop-butyl，metamifop，bispyribac-sodium，pyriftalid， ，氯氟乙氧基，氧氟芬，二硫代吡啶，MCPA，MCPB，2,4-D，胞嘧啶，异戊肾上腺素，喹诺酮和克罗莫他， d /或（c）一种或多种选自安息剂dymron，异恶草酮（ - 乙基），氟尿嘧啶，芬氯唑，芬舍洛芬，氯喹毒素，奥沙利坦，氟吡芬，咪芬吡啶二乙基，呋喃唑，R-29148 （代号），benoxacor，dichlormid和dicyclonone作为有效成分。

公开(公告)号：US07829322B2

公开(公告)日：2010-11-09

申请号：US11722162

申请日：2005-12-20

申请人： Takeko Kodama ,  Keiji Endo ,  Katsuya Ozaki ,  Junichi Sekiguchi

发明人： Takeko Kodama ,  Keiji Endo ,  Katsuya Ozaki ,  Junichi Sekiguchi

IPC分类号： C12N1/21 ,  C12N15/00 ,  C12N15/57 ,  C12N15/75

CPC分类号： C12P21/02 ,  C07K14/32 ,  C12N9/54 ,  C12N15/75

摘要： A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided.Also provided is a recombinant microorganism obtained by introducing into a host microorganism a gene encoding a heterologous protein or polypeptide, wherein in said host microorganism the Bacillus subtilis aprX gene or a gene corresponding to the aprX gene has been deleted or knocked out, and a method for producing a protein or polypeptide using the recombinant microorganism.

摘要翻译： 提供能够提高蛋白质或多肽的生产力的宿主微生物，通过将编码蛋白质或多肽的基因导入宿主微生物而获得的重组微生物，以及使用该重组微生物产生蛋白质或多肽的方法。 还提供了通过向宿主微生物中导入编码异源蛋白质或多肽的基因而获得的重组微生物，其中在所述宿主微生物中，枯草芽孢杆菌aprX基因或与aprX基因相对应的基因已被缺失或敲除， 用于使用重组微生物产生蛋白质或多肽。

公开(公告)号：US20090170154A1

公开(公告)日：2009-07-02

申请号：US10590275

申请日：2005-03-04

申请人： Keiji Endo ,  Katsuya Ozaki

发明人： Keiji Endo ,  Katsuya Ozaki

IPC分类号： C12P21/00 ,  C12N1/21 ,  C12N15/87

CPC分类号： C12N9/2417 ,  C12N9/2437 ,  C12N9/54 ,  C12Y302/01004

摘要： The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.

摘要翻译： 本发明提供能够提高蛋白质或多肽生产力的突变芽孢杆菌属细菌，通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌产生的重组微生物，以及通过使用重组微生物产生蛋白质或多肽的方法。 一种突变芽孢杆菌属细菌，其在基因组或质粒上具有在孢子形成阶段中特异性识别并转录并与sigA基因或其等同基因相连的基因的上游端的启动子序列的DNA，重组微生物 通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌而产生，以及通过使用重组微生物产生蛋白质或多肽的方法。

公开(公告)号：US20080026976A1

公开(公告)日：2008-01-31

申请号：US11590769

申请日：2006-11-01

申请人： Hiroyuki Araki ,  Keiji Endo ,  Hiroshi Hagihara ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Katsuya Ozaki

发明人： Hiroyuki Araki ,  Keiji Endo ,  Hiroshi Hagihara ,  Kazuaki Igarashi ,  Yasuhiro Hayashi ,  Katsuya Ozaki

IPC分类号： C11D3/386 ,  C07H21/00 ,  C12N1/00 ,  C12N15/63 ,  C12N9/26 ,  C12P21/00

CPC分类号： C12N9/2417 ,  C11D3/386

摘要： The invention relates to mutant α-amylases that may be produced at high yield from recombinant microorganisms.

摘要翻译： 本发明涉及可以从重组微生物以高产率产生的突变型α-淀粉酶。

公开(公告)号：US07033981B2

公开(公告)日：2006-04-25

申请号：US10383630

申请日：2003-03-10

申请人： Yoshihiro Hakamada ,  Kazuhisa Sawada ,  Keiji Endo ,  Hiroshi Kodama ,  Yasunao Wada ,  Shitsu Shikata ,  Tohru Kobayashi

发明人： Yoshihiro Hakamada ,  Kazuhisa Sawada ,  Keiji Endo ,  Hiroshi Kodama ,  Yasunao Wada ,  Shitsu Shikata ,  Tohru Kobayashi

IPC分类号： C11D3/386 ,  C12N9/42 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12Y302/01004

摘要： The present invention relates to alkaline cellulase variants having an amino acid sequence which exhibits at least 95% homology with the amino acid sequence of SEQ ID NO: 2, wherein said alkaline cellulase variant is mutated to include at least one substitution at (a) position 10, (b) position 16, (c) position 22, (d) position 33, (e) position 39, (f) position 76, (g) position 109, (h) position 242, (i) position 263, (j) position 308, (k) position 462, (l) position 466, (m) position 468, (n) position 552, (o) position 564, or (p) position 608 in SEQ ID NO: 2, or at a position corresponding thereto, wherein said alkaline cellulase variant has alkaline cellulase activity. The present invention also relates to a gene encoding the variant; a vector containing the gene; a transformant containing the vector; and a detergent composition containing the alkaline cellulase variant.

摘要翻译： 本发明涉及具有与SEQ ID NO：2的氨基酸序列具有至少95％同源性的氨基酸序列的碱性纤维素酶变体，其中所述碱性纤维素酶变体突变以在（a）位置包括至少一个取代 10，（b）位置16，（c）位置22，（d）位置33，（e）位置39，（f）位置76，（g）位置109，（h）位置242，（i）位置263， （j）位置308，（k）SEQ ID NO：2中的位置462，（l）位置466，（m）位置468，（n）位置552，（o）位置564或（p）位置608，或 在相应的位置，其中所述碱性纤维素酶变体具有碱性纤维素酶活性。 本发明还涉及编码该变体的基因; 含有该基因的载体; 含有载体的转化体 和含有碱性纤维素酶变体的洗涤剂组合物。

公开(公告)号：US5081757A

公开(公告)日：1992-01-21

申请号：US697197

申请日：1991-05-08

申请人： Atsushi Sato ,  Shigenobu Kawakami ,  Keiji Endo ,  Hideyuki Dohi

发明人： Atsushi Sato ,  Shigenobu Kawakami ,  Keiji Endo ,  Hideyuki Dohi

IPC分类号： H01B3/22 ,  H01B3/44

CPC分类号： H01B3/44 ,  H01B3/22 ,  Y10T29/43

摘要： An electrical insulating oil composition having good low temperature characteristics which comprises at least 4 members selected from the group consisting of (a) m-ethylbiphenyl, (b) p-ethylbiphenyl, (c) o-benzyltoluene, (d) m-benzyltoluene, (e) p-benzyltoluene, (f) 1,1-diphenylethane, and (g) 1,1-diphenylethylene; and is characterized in that the proportion of solid phase at a temperature of -40.degree. C. of the electrical insulating oil composition is not more than 45% by weight and the proportion of the total quantity of solid phase is calculated according to the following general equation of solid-liquid equilibrium: ##EQU1## wherein X.sub.i is the equilibrium mole fraction of a component i in the liquid phase of the composition, .DELTA.H.sub.i.sup.f is the heat of fusion (cal.mol.sup.-1), T.sub.i.sup.f is the melting point (K), T is the temperature (K), and R is the gas constant (cal.mol.sup.-1.K.sup.-1).Electrical capacitors prepared with these electrical insulating oil compositions provide excellent operation at temperatures as low as -40.degree. C., preferably as low as -50.degree. C.

公开(公告)号：US4899009A

公开(公告)日：1990-02-06

申请号：US364490

申请日：1989-06-09

申请人： Shigenobu Kawakami ,  Keiji Endo ,  Hideyuki Dohi ,  Atsushi Sato

发明人： Shigenobu Kawakami ,  Keiji Endo ,  Hideyuki Dohi ,  Atsushi Sato

IPC分类号： B01J29/40 ,  C07B61/00 ,  C07C1/00 ,  C07C6/12 ,  C07C15/14 ,  C07C15/16 ,  C07C67/00

CPC分类号： C07C6/12 ,  C07C2529/40

摘要： A method for producing m-benzyltoluene with a high yield and excellent selectivity without producing undesirable heavier by-products. The method is characterized in that toluene and diphenylmethane are allowed to react at a reaction temperature in the range of 170.degree. to 400.degree. C. in the presence of a crystalline synthetic zeolite catalyst in which the molar ratio of SiO.sub.2 /Al.sub.2 O.sub.3 is 20 or higher and the openings of main pores are formed by ten-membered oxygen rings.

公开(公告)号：US4659365A

公开(公告)日：1987-04-21

申请号：US769123

申请日：1985-08-26

申请人： Kazuhiko Konno ,  Koichi Araki ,  Kaoru Ikeda ,  Keiji Endo ,  Mitsuru Hikido

发明人： Kazuhiko Konno ,  Koichi Araki ,  Kaoru Ikeda ,  Keiji Endo ,  Mitsuru Hikido

IPC分类号： A01N37/24 ,  A01N37/26 ,  A01N43/56 ,  C07C233/88 ,  C07D231/12 ,  C07D521/00 ,  C07C103/375

CPC分类号： C07D231/12 ,  A01N37/24 ,  A01N37/26 ,  A01N43/56 ,  C07D233/56 ,  C07D249/08

摘要： An acetanilide derivative of the following formula (I) and a herbicide containing one or more of the acetanilide derivative as the active ingredient are described. ##STR1## In the formula, R.sup.1 is a lower alkyl group; R.sup.2 is a fluorine-substituted lower alkyl group; A is ##STR2## B is an optionally substituted pyrazolyl group, an optionally substituted alkoxy group or a hydrogen atom.The acetanilide derivative and the herbicide have a selective weed killing activity to gramiveous weeds.

摘要翻译： 描述下式（I）的乙酰苯胺衍生物和含有一种或多种乙酰苯胺衍生物作为活性成分的除草剂。 在该式中，R 1为低级烷基; R2是氟取代的低级烷基; A是B是任选取代的吡唑基，任选取代的烷氧基或氢原子。 乙酰苯胺衍生物和除草剂对杂草杂草具有选择性除草活性。