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22 条记录 (耗时 0.046 秒)

    Method of quantitating genomic DNA
    11.
    发明授权
    Method of quantitating genomic DNA 失效
    定量基因组DNA的方法

    公开(公告)号：US5858658A

    公开(公告)日：1999-01-12

    申请号：US533967

    申请日：1995-09-26

    申请人： Thomas Haemmerle ,  Falko-Guenter Falkner ,  Johann Kohl ,  Michele Himmelspach ,  Friedrich Dorner

    发明人： Thomas Haemmerle ,  Falko-Guenter Falkner ,  Johann Kohl ,  Michele Himmelspach ,  Friedrich Dorner

    IPC分类号： G01N33/50 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/70

    CPC分类号： C12Q1/6876 ,  C12Q1/6851

    摘要： A method of quantitating genomic DNA in a sample is provided. The method comprises the steps of adding to the sample a given amount of at least one nucleic acid as an internal standard, wherein the standard nucleic acid differs from the genomic DNA to be quantified in at least one detectable characteristic; amplifying the genomic DNA and the internal standard nucleic acid by means of a nucleic acid amplification process employing primers complementary to repetitive genomic sequences; determining as a first amount the amount of amplified genomic DNA, and determining as a second amount the amount of amplified standard nucleic acid; and determining from the first and second amounts, as a third amount, the amount of genomic DNA originally contained in the sample. Kits for performing the method and products substantially free of foreign DNA as determined by the method also are provided.

    摘要翻译： 提供了一种定量样品中基因组DNA的方法。 该方法包括以下步骤：向样品中加入给定量的至少一种作为内标的核酸，其中标准核酸与待定量的至少一种可检测特征的基因组DNA不同; 通过使用与重复基因组序列互补的引物的核酸扩增方法扩增基因组DNA和内标核酸; 确定扩增的基因组DNA的量作为第一量，并将第二量确定为扩增的标准核酸的量; 并且从第一和第二量确定作为第三量，最初包含在样品中的基因组DNA的量。 还提供了通过该方法确定的用于执行基本上不含外来DNA的方法和产物的试剂盒。

    Method of quantitating nucleic acid
    12.
    发明授权
    Method of quantitating nucleic acid 失效
    定量核酸的方法

    公开(公告)号：US5789153A

    公开(公告)日：1998-08-04

    申请号：US533820

    申请日：1995-09-26

    申请人： Falko-Guenter Falkner ,  Thomas Haemmerle ,  Michele Himmelspach ,  Johann Kohl ,  Friedrich Dorner

    发明人： Falko-Guenter Falkner ,  Thomas Haemmerle ,  Michele Himmelspach ,  Johann Kohl ,  Friedrich Dorner

    IPC分类号： G01N33/58 ,  A61K39/00 ,  A61K39/12 ,  C07H21/04 ,  C12N15/09 ,  C12P21/08 ,  C12Q1/68 ,  C12Q1/70 ,  C12P19/34 ,  C07H21/02

    CPC分类号： C12Q1/701 ,  C12Q1/6851

    摘要： There is disclosed a method of quantitating nucleic acids in a sample by using nucleic acid amplification, wherein, prior to the amplification step, a given amount of a known nucleic acid molecule is added to the sample as internal standard, which standard nucleic acid molecule differs from the nucleic acid to be quantitated by at least one detectable characteristic; to obtain a high precision and good reproducibility, known amounts of at least two known nucleic acid molecules which differ from each other and from the nucleic acid to be quantitated in at least one detectable characteristic are added as an internal standard to the sample prior to nucleic acid amplification, the amounts of amplified sample and standard nucleic acids obtained are determined, and from the amounts obtained, the amount of the nucleic acid to be quantitated originally present in the sample is determined.

    摘要翻译： 公开了通过使用核酸扩增来定量样品中的核酸的方法，其中在扩增步骤之前，将给定量的已知核酸分子作为内标添加到样品中，该标准核酸分子不同 从要由至少一个可检测特征定量的核酸; 为了获得高精度和良好的再现性，将已知量的至少两种彼此不同的已知核酸分子和从至少一个可检测特征定量的核酸作为内标物加入到核酸前的样品中 酸扩增，测定所获得的扩增样品和标准核酸的量，从得到的量确定原样存在于样品中的待定量核酸的量。

    Production of Viral Vaccine
    16.
    发明申请
    Production of Viral Vaccine 有权
    生产病毒疫苗

    公开(公告)号：US20100189745A1

    公开(公告)日：2010-07-29

    申请号：US12639917

    申请日：2009-12-16

    申请人： Otfried Kistner ,  Falko-Guenter Falkner ,  Hartmut Ehrlich ,  Noel Barrett

    发明人： Otfried Kistner ,  Falko-Guenter Falkner ,  Hartmut Ehrlich ,  Noel Barrett

    IPC分类号： A61K39/145 ,  C12N7/00

    CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/5258 ,  C12N7/00 ,  C12N2760/16134 ,  C12N2760/16161

    摘要： The present invention relates, in general, to materials and methods for production of an improved vaccine against influenza virus, wherein the vaccine comprises a reassortant virus having a hemagglutinin gene and a neuraminidase gene from the same influenza A virus subtype or influenza B strain of virus and internal genes from a different influenza A virus subtype or influenza B strain of virus. In one aspect, the HA and NA genes and the internal genes are from a highly pathogenic H5N1 strain of influenza A.

    摘要翻译： 本发明一般涉及用于生产针对流感病毒的改进疫苗的材料和方法，其中所述疫苗包含具有血凝素基因的重配病毒和来自相同甲型流感病毒亚型或乙型流感病毒的病毒的神经氨酸酶基因 和来自不同甲型流感病毒亚型或乙型流感病毒的内部基因。 一方面，HA和NA基因和内部基因来自流感A的高致病性H5N1毒株。