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92 条记录 (耗时 0.039 秒)

    Method for determining azole resistance in Candida glabrata
    11.
    发明授权
    Method for determining azole resistance in Candida glabrata 有权
    确定念珠菌的唑类抗性的方法

    公开(公告)号：US08021846B2

    公开(公告)日：2011-09-20

    申请号：US12381492

    申请日：2009-03-12

    申请人： Scott E. Gygax ,  John-Paul Vermitsky ,  Sean G. Chadwick ,  Matthew J. Self ,  Eli Mordechai ,  Martin E. Adelson

    发明人： Scott E. Gygax ,  John-Paul Vermitsky ,  Sean G. Chadwick ,  Matthew J. Self ,  Eli Mordechai ,  Martin E. Adelson

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6895 ,  C12Q2600/158 ,  C12Q2600/16

    摘要： There is disclosed a method for determining azole resistance in Candida glabrata. A biological sample containing Candida glabrata is obtained and a normalized mRNA level of CDR1 gene is determined using qRT-PCR. Using a microbroth dilution assay conducted at azole concentrations of about 2-8 μg/mL, a susceptible isolate of Candida glabrata is obtained. A qRT-PCR assay is employed on the susceptible isolate and an average mRNA level of CDR1 is obtained. A fold-change value for CDR1 is obtained by comparing the CDR1 mRNA level of the biological sample with that of the average mRNA level. A ≧2-fold change value is indicative of an azole resistance in Candida glabrata. The present method provides a qRT-PCR assay for azole resistance that has a sensitivity of ≧90% and a specificity of ≧90%.

    摘要翻译： 公开了一种测定念珠菌的唑类抗性的方法。 获得含有念珠菌的生物样品，并使用qRT-PCR测定CDR1基因的归一化mRNA水平。 使用在约2-8μg/ mL的唑浓度下进行的微量稀释测定，获得了易裂片假丝酵母（Candida glabrata）的分离物。 在易感性分离株上使用qRT-PCR测定，获得CDR1的平均mRNA水平。 通过将生物样品的CDR1 mRNA水平与平均mRNA水平的CDR1 mRNA水平进行比较，获得CDR1的倍数值。 A≥2倍变化值表示光滑念珠菌中的唑类抗性。 本方法提供具有≥90％的灵敏度和≥90％的特异性的唑抗性的qRT-PCR测定。

    Method for determining azole resistance in candida glabrata
    12.
    发明申请
    Method for determining azole resistance in candida glabrata 有权
    确定念珠菌的唑类抗性的方法

    公开(公告)号：US20090305274A1

    公开(公告)日：2009-12-10

    申请号：US12381492

    申请日：2009-03-12

    申请人： Scott E. Gygax ,  John-Paul Vermitsky ,  Sean G. Chadwick ,  Matthew J. Self ,  Eli Mordechai ,  Martin E. Adelson

    发明人： Scott E. Gygax ,  John-Paul Vermitsky ,  Sean G. Chadwick ,  Matthew J. Self ,  Eli Mordechai ,  Martin E. Adelson

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6895 ,  C12Q2600/158 ,  C12Q2600/16

    摘要： There is disclosed a method for determining azole resistance in Candida glabrata. A biological sample containing Candida glabrata is obtained and a normalized mRNA level of CDR1 gene is determined using qRT-PCR. Using a microbroth dilution assay conducted at azole concentrations of about 2-8 μg/mL, a susceptible isolate of Candida glabrata is obtained. A qRT-PCR assay is employed on the susceptible isolate and an average mRNA level of CDR1 is obtained. A fold-change value for CDR1 is obtained by comparing the CDR1 mRNA level of the biological sample with that of the average mRNA level. A ≧2-fold change value is indicative of an azole resistance in Candida glabrata. The present method provides a qRT-PCR assay for azole resistance that has a sensitivity of ≧90% and a specificity of ≧90%.

    摘要翻译： 公开了一种测定念珠菌的唑类抗性的方法。 获得含有念珠菌的生物样品，并使用qRT-PCR测定CDR1基因的标准化mRNA水平。 使用在约2-8mug / mL的唑浓度下进行的微量级稀释测定法，获得了易裂片假丝酵母（Candida glabrata）的分离物。 在易感性分离株上使用qRT-PCR测定，获得CDR1的平均mRNA水平。 通过将生物样品的CDR1 mRNA水平与平均mRNA水平的CDR1 mRNA水平进行比较，获得CDR1的倍数值。 A> = 2倍变化值表示光滑念珠菌中的唑类抗性。 本方法提供灵敏度> = 90％，特异性> = 90％的唑类抗性的qRT-PCR测定。

    Methods and compositions related thereto for detecting and identifying distinct species of nucleic acids from causative agents
    16.
    发明授权
    Methods and compositions related thereto for detecting and identifying distinct species of nucleic acids from causative agents 有权
    与之相关的方法和组合物用于检测和鉴定来自致病因子的不同种类的核酸

    公开(公告)号：US08932832B2

    公开(公告)日：2015-01-13

    申请号：US13199380

    申请日：2011-08-26

    申请人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

    发明人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

    IPC分类号： C07H21/04 ,  C12Q1/70

    CPC分类号： C12Q1/705

    摘要： Methods are described herein for detecting and identifying distinct species of nucleic acids, in a single container, for example, from a certain genus of infectious agents or otherwise causative agents comprising, for example, providing a forward PCR primer common to a homologous gene region between the distinct species, and providing a reverse PCR primer common to a homologous gene region between the distinct species, to thereby define a PCR target region amongst the species, and providing a first oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a first species, providing a second oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a second species, wherein the first and second oligonucleotide probes are each detectably labeled with distinctly different detectable labels, conducting a PCR reaction in the container by means of the primers to amplify the target region amongst the species, and detecting the distinct labels, thereby identifying distinct species of nucleic acids corresponding to distinct species of infectious agents. Methods are preferred, for example, wherein the infectious agent is a member of the Herpesviridae family.

    摘要翻译： 在本文中描述了用于在单个容器中，例如来自某一感染因子或其他致病因子的单个容器中检测和鉴定不同种类的核酸的方法，所述诱导剂包括例如提供在同源基因区域之间共同的正向PCR引物 并且提供与不同物种之间的同源基因区域共有的反向PCR引物，从而在物种间限定PCR靶区域，并提供对靶区域内的核酸序列特异的第一寡核苷酸探针， 提供对目标区域内具有特征的第二种类的核酸序列特异性的第二寡核苷酸探针，其中第一和第二寡核苷酸探针各自可检测地用明显不同的可检测标记物标记，进行PCR反应 在容器中通过引物扩增目标区域amo 并检测不同的标签，从而识别与不同种类的感染因子相对应的不同种类的核酸。 方法是优选的，例如，其中感染剂是疱疹病毒科家族的成员。

    Method of determining types I, II, III, IV or V or methicillin-resistant staphylococcus aureus (MRSA) in a biological sample
    19.
    发明申请
    Method of determining types I, II, III, IV or V or methicillin-resistant staphylococcus aureus (MRSA) in a biological sample 有权
    在生物样品中测定I，II，III，IV或V型或耐甲氧西林金黄色葡萄球菌（MRSA）的方法

    公开(公告)号：US20110312876A1

    公开(公告)日：2011-12-22

    申请号：US12930663

    申请日：2011-01-13

    申请人： Scott Elliot Gygax ,  Christina Lim Overmyer ,  Lisa A. DeSalvia ,  Martin E. Adelson ,  Eli Mordechai

    发明人： Scott Elliot Gygax ,  Christina Lim Overmyer ,  Lisa A. DeSalvia ,  Martin E. Adelson ,  Eli Mordechai

    IPC分类号： A61K38/14 ,  A61P31/04 ,  C12Q1/68 ,  A61K31/5377 ,  C40B30/00 ,  C07H21/04

    CPC分类号： C12Q1/689 ,  A61K31/5377 ,  A61K38/12 ,  A61K38/14 ,  C12Q1/6844 ,  C12Q2600/16

    摘要： Disclosed are diagnostic methods for determining a subtype of methicillin-resistant Staphylococcus aureus (MRSA) in a biological sample of a mammal. Methods include providing a biological sample of the mammal, performing a PCR analysis of the biological sample, and analyzing the PCR amplicons with respect to their sizes so as to determine for type I, type II, type III, type IV or type V MRSA that may be present in the biological sample. Further example embodiments include using at least one mecA primer pair and/or using at least one Staphylococcus aureus nuc primer pair in the PCR analysis. Further disclosed are methods for screening populations for MRSA, and methods of treating a mammal testing positive for Type IV MRSA. Also disclosed are kits for determining a MRSA subtype in a mammal and isolated primers that may be used in the present methods and kits.

    摘要翻译： 公开了用于确定哺乳动物生物样品中耐甲氧西林金黄色葡萄球菌（MRSA）亚型的诊断方法。 方法包括提供哺乳动物的生物样品，对生物样品进行PCR分析，并根据其大小对PCR扩增子进行分析，以确定I型，II型，III型，IV型或V型MRSA， 可能存在于生物样品中。 另外的实施例包括在PCR分析中使用至少一个mecA引物对和/或使用至少一种金黄色葡萄球菌引物对。 进一步披露的是用于筛选MRSA群体的方法以及治疗IV型MRSA阳性的哺乳动物的方法。 还公开了用于测定哺乳动物中MRSA亚型的试剂盒和可用于本发明方法和试剂盒的分离的引物。