公开(公告)号：US09447426B2

公开(公告)日：2016-09-20

申请号：US13639522

申请日：2011-03-30

申请人： Sachihiro Matsunaga ,  Tsutomu Kohinata ,  Kiichi Fukui ,  Satoshi Tabata

发明人： Sachihiro Matsunaga ,  Tsutomu Kohinata ,  Kiichi Fukui ,  Satoshi Tabata

IPC分类号： C12N15/82 ,  C07K14/415

CPC分类号： C12N15/8273 ,  C07K14/415

摘要： By analyzing a Jatropha genome, NF-YB-encoding genes of SEQ ID NOs: 1 to 11, fragments of NF-YB-encoding genes of SEQ ID NOs: 12 and 13, and genes relating thereto were found. By transforming Jatropha with these NF-YB-encoding genes and the like, it is possible to overexpress a NF-YB polypeptide and so on, and to significantly improve the productivity of protein synthesis involved by the NF-YB polypeptide, and to significantly improve the dry stress resistance, for example. As a result, it is possible to create dry stress resistant Jatropha capable of ensuring high growth even under water deficient conditions.

摘要翻译： 通过分析麻风树基因组，发现SEQ ID NO：1至11的NF-YB编码基因，SEQ ID NO：12和13的NF-YB编码基因的片段，以及与之相关的基因。 通过使用这些NF-YB编码基因等将麻疯树进行转化，可以过表达NF-YB多肽等，并显着提高NF-YB多肽所涉及的蛋白质合成的生产力，并显着提高 耐干性，例如。 因此，即使在缺水条件下，也可以产生能够确保高生长的耐干旱麻疯树。

公开(公告)号：US09255278B2

公开(公告)日：2016-02-09

申请号：US13643396

申请日：2011-10-25

申请人： Kiichi Fukui ,  Joyce Cartagena ,  Naoki Wada ,  Tsutomu Kohinata ,  Yasuhisa Yushio ,  Naoki Ikeguchi ,  Satoshi Tabata

发明人： Kiichi Fukui ,  Joyce Cartagena ,  Naoki Wada ,  Tsutomu Kohinata ,  Yasuhisa Yushio ,  Naoki Ikeguchi ,  Satoshi Tabata

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N9/06 ,  C12N15/53 ,  C12N9/12

CPC分类号： C12N15/8247 ,  C12N9/1241 ,  C12N15/8243 ,  C12N15/8271 ,  C12N15/8273 ,  C12Y207/07003

摘要： A PPAT polypeptide of SEQ ID NO: 1 derived from Jatropha, a PPAT polynucleotide of SEQ ID NO: 2 and so on were found. By transforming Jatropha with these PPAT polynucleotides, it is possible to overexpress the PPAT polypeptide in comparison with a wild type, and biosynthesis of coenzyme A is promoted by these polypeptides, the metabolic function and viability of the transformed Jatropha are enhanced, and for example, stress resistance can be significantly improved.

摘要翻译： 发现衍生自麻疯树的SEQ ID NO：1的PPAT多肽，SEQ ID NO：2的PPAT多核苷酸等。 通过用这些PPAT多核苷酸转化麻疯树，可以与野生型相比过表达PPAT多肽，并且这些多肽促进了辅酶A的生物合成，增强了转化的麻疯树的代谢功能和活力，例如， 耐应力可显着提高。

公开(公告)号：US20050279928A1

公开(公告)日：2005-12-22

申请号：US11151466

申请日：2005-06-14

申请人： Kunihiko Ohkubo ,  Kiichi Fukui ,  Kazuyoshi Itoh

发明人： Kunihiko Ohkubo ,  Kiichi Fukui ,  Kazuyoshi Itoh

IPC分类号： H01J49/16 ,  H01J49/40 ,  B01D59/44

CPC分类号： H01J49/162 ,  H01J49/0463

摘要： The mass spectrometer according to the present invention includes a light source for emitting pulse light including a plurality of wavelengths; an ionizer for ionizing molecules of a sample by irradiating the light from the light source to the sample; and a mass analyzer for separating ions ionized in the ionizer according to their mass to charge ratios. For the light source, one including a plurality of ultrashort pulse laser sources each emitting a wavelength different from others, and one emitting ultrashort pulse light including plural wavelengths ranging from the visible region to the infrared region generated by dispersing an ultrashort pulse light with continuous (white) spectrum can be used. Pulse lights having plural wavelengths ranging from near infrared to the ultraviolet region respectively share the role; i.e., one of them vaporizes the sample without fragmenting it, and another ionizes the vaporized sample with the single-photon process or two-photon (or multi-photon) process. This enables ionization of protein complexes as a whole contained in the sample, and enables mass analyses of them.

摘要翻译： 根据本发明的质谱仪包括用于发射包括多个波长的脉冲光的光源; 电离器，用于通过将来自光源的光照射到样品上来使样品的分子离子化; 以及质量分析器，用于根据电离质量与电离比分离在离子发生器中离子化的离子。 对于光源，包括多个发射不同于其他波长的超短脉冲激光源的光源，以及一个发射包括从可见光区域到红外区域的多个波长的超短脉冲光，通过分散具有连续的超短脉冲光 白色）光谱。 分别具有从近红外到紫外线区域的多个波长的脉冲光共同作用; 即，其中一个使样品蒸发而不使其分裂，另一个使用单光子过程或双光子（或多光子）过程将蒸发的样品电离。 这使得能够将样品中包含的蛋白质复合物电离，并且能够进行质量分析。

公开(公告)号：US06596540B2

公开(公告)日：2003-07-22

申请号：US09956166

申请日：2001-09-20

申请人： Kiichi Fukui ,  Akio Kobayashi ,  Satoshi Harashima ,  Eiichiro Fukusaki ,  Takefumi Sone

发明人： Kiichi Fukui ,  Akio Kobayashi ,  Satoshi Harashima ,  Eiichiro Fukusaki ,  Takefumi Sone

IPC分类号： C12N1500

CPC分类号： C12N15/89

摘要： A novel method for introduction of an exogenous substance or a physiologically active compound into cells is provided according to this invention. This method can realize introduction of an exogenous genetic substance or a physiologically active compound of large size with a large amount. Such substance is immobilized to beads of sphere fine particles having a particle size of 0.01 mm to 10 mm, and bio-active beads thus produced are introduced into cells. Bio-active beads comprising calcium alginate are particularly useful for the purpose of the present invention.

摘要翻译： 根据本发明提供了将外源物质或生理活性化合物引入细胞的新方法。 该方法可以实现大量外源遗传物质或生理活性化合物的引入。 将该物质固定在粒径为0.01mm〜10mm的球状微粒子珠上，将生成的生物活性珠子导入到细胞中。 包含藻酸钙的生物活性珠特别适用于本发明的目的。

公开(公告)号：US08629980B2

公开(公告)日：2014-01-14

申请号：US13375388

申请日：2010-06-02

申请人： Yasuyuki Ozeki ,  Kazuyoshi Itoh ,  Fumihiro Dake ,  Kiichi Fukui ,  Shinichiro Kajiyama ,  Yuma Kitagawa ,  Norihiko Nishizawa ,  Kazuhiko Sumimura

发明人： Yasuyuki Ozeki ,  Kazuyoshi Itoh ,  Fumihiro Dake ,  Kiichi Fukui ,  Shinichiro Kajiyama ,  Yuma Kitagawa ,  Norihiko Nishizawa ,  Kazuhiko Sumimura

IPC分类号： G01J3/44

CPC分类号： G01J3/4412 ,  G01N21/65 ,  G02B21/06 ,  G02B21/365

摘要： The invention provides an optical microscope that prevents an increase in the complexity of the light source system and is equipped with optics readily capable of adequate operation even when the modulation frequency is increased in order to reduce the impact of the intensity noise of the laser, etc. This optical microscope 100 irradiates a sample 6 with a first train of optical pulses having a first optical frequency, which is generated by a first light source, and a second train of optical pulses having a second optical frequency, which is temporally synchronized with the first train of optical pulses and is generated by a second light source, and detects light scattered from the sample 6. The repetition frequency of the train of optical pulses generated by the first light source is an integral sub-multiple of the repetition frequency of the train of optical pulses generated by the second light source.

摘要翻译： 本发明提供了一种光学显微镜，其防止光源系统的复杂性的增加，并且配备有即使当增加调制频率以便减少激光器的强度噪声的影响等时容易进行适当操作的光学器件 该光学显微镜100照射具有由第一光源产生的具有第一光学频率的第一列光脉冲的样品6和具有与第二光频同步的第二光学频率的第二光脉冲序列 第一光脉冲串，并由第二光源产生，并且检测从样品6散射的光。由第一光源产生的光脉冲序列的重复频率是该第一光源的重复频率的整数倍 由第二光源产生的光脉冲序列。

公开(公告)号：US20130072702A1

公开(公告)日：2013-03-21

申请号：US13639522

申请日：2011-03-30

申请人： Sachihiro Matsunaga ,  Tsutomu Kohinata ,  Kiichi Fukui ,  Satoshi Tabata

发明人： Sachihiro Matsunaga ,  Tsutomu Kohinata ,  Kiichi Fukui ,  Satoshi Tabata

IPC分类号： C11B1/06 ,  C12N15/82 ,  A01H5/10 ,  C07K14/415 ,  A01H5/00 ,  C12N15/29 ,  C12N5/10

CPC分类号： C12N15/8273 ,  C07K14/415

摘要： By analyzing a Jatropha genome, NF-YB-encoding genes of SEQ ID NOs: 1 to 11, fragments of NF-YB-encoding genes of SEQ ID NOs: 12 and 13, and genes relating thereto were found. By transforming Jatropha with these NF-YB-encoding genes and the like, it is possible to overexpress a NF-YB polypeptide and so on, and to significantly improve the productivity of protein synthesis involved by the NF-YB polypeptide, and to significantly improve the dry stress resistance, for example. As a result, it is possible to create dry stress resistant Jatropha capable of ensuring high growth even under water deficient conditions.

摘要翻译： 通过分析麻风树基因组，发现SEQ ID NO：1至11的NF-YB编码基因，SEQ ID NO：12和13的NF-YB编码基因的片段，以及与之相关的基因。 通过使用这些NF-YB编码基因等将麻疯树进行转化，可以过表达NF-YB多肽等，并显着提高NF-YB多肽所涉及的蛋白质合成的生产力，并显着提高 耐干性，例如。 因此，即使在缺水条件下，也可以产生能够确保高生长的耐干旱麻疯树。

公开(公告)号：US20120140217A1

公开(公告)日：2012-06-07

申请号：US13375388

申请日：2010-06-02

申请人： Yasuyuki Ozeki ,  Kazuyoshi Itoh ,  Fumihiro Dake ,  Kiichi Fukui ,  Shinichiro Kajiyama ,  Yuma Kitagawa ,  Norihiko Nishizawa ,  Kazuhiko Sumimura

发明人： Yasuyuki Ozeki ,  Kazuyoshi Itoh ,  Fumihiro Dake ,  Kiichi Fukui ,  Shinichiro Kajiyama ,  Yuma Kitagawa ,  Norihiko Nishizawa ,  Kazuhiko Sumimura

IPC分类号： G01N21/65

CPC分类号： G01J3/4412 ,  G01N21/65 ,  G02B21/06 ,  G02B21/365

摘要： The invention provides an optical microscope that prevents an increase in the complexity of the light source system and is equipped with optics readily capable of adequate operation even when the modulation frequency is increased in order to reduce the impact of the intensity noise of the laser, etc. This optical microscope 100 irradiates a sample 6 with a first train of optical pulses having a first optical frequency, which is generated by a first light source, and a second train of optical pulses having a second optical frequency, which is temporally synchronized with the first train of optical pulses and is generated by a second light source, and detects light scattered from the sample 6. The repetition frequency of the train of optical pulses generated by the first light source is an integral sub-multiple of the repetition frequency of the train of optical pulses generated by the second light source.

摘要翻译： 本发明提供了一种光学显微镜，其防止光源系统的复杂性的增加，并且配备有即使当增加调制频率以便减少激光器的强度噪声的影响等时容易进行适当操作的光学器件 该光学显微镜100照射具有由第一光源产生的具有第一光学频率的第一列光脉冲的样品6和具有与第二光频同步的第二光学频率的第二光脉冲序列 第一光脉冲串，并由第二光源产生，并且检测从样品6散射的光。由第一光源产生的光脉冲序列的重复频率是该第一光源的重复频率的整数倍 由第二光源产生的光脉冲序列。

公开(公告)号：US07989588B2

公开(公告)日：2011-08-02

申请号：US11883810

申请日：2006-02-09

申请人： Shinsaku Nakagawa ,  Tadanori Mayumi ,  Kiichi Fukui

发明人： Shinsaku Nakagawa ,  Tadanori Mayumi ,  Kiichi Fukui

IPC分类号： C07K7/08 ,  C07K19/00 ,  A61K47/48

CPC分类号： C40B40/02 ,  A61K39/385 ,  A61K47/62 ,  A61K47/645 ,  A61K47/646 ,  A61K2039/6031 ,  C07K7/06 ,  C07K7/08 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/735 ,  C12N15/1037 ,  C12N2740/16322

摘要： The number of peptides having an ability to bind to a cell or penetrate into a cell is narrowed down by being selectively enriched from a random peptide library with a diversity of not less than one hundred millions of peptides using a phage surface display technique, and then cytoplasmic transfer is evaluated by using protein synthesis inhibition as an indicator by adding to a cell, a fusion body of the selectively enriched peptide and a protein synthesis inhibitory factor (PSIF) that cannot solely penetrate into the cell.

摘要翻译： 通过使用噬菌体表面显示技术从具有不少于一亿多种肽的多样性的随机多肽文库中选择性富集具有结合细胞或穿透细胞的能力的肽的数量变窄，然后 通过添加细胞，选择性富集的肽的融合体和蛋白质合成抑制因子（PSIF）不能仅仅渗入细胞，通过使用蛋白质合成抑制作为指标来评价细胞质转移。

公开(公告)号：US06864975B2

公开(公告)日：2005-03-08

申请号：US10310032

申请日：2002-12-05

申请人： Kazuyoshi Itoh ,  Kiichi Fukui ,  Kunihiko Ohkubo

发明人： Kazuyoshi Itoh ,  Kiichi Fukui ,  Kunihiko Ohkubo

IPC分类号： G01J3/10 ,  G01J3/44 ,  G01N21/01 ,  G01N21/64 ,  G01J3/18

CPC分类号： G01J3/4406 ,  G01J3/0229 ,  G01J3/10 ,  G01J2003/1213 ,  G01N21/645 ,  G01N2021/6419

摘要： The present invention proposes a method of and system for analyzing samples (particularly, bio-samples), wherein the continuous spectrum white light pulse generated from an ultra-short optical pulse is used in a simple manner. By a system according to the invention, continuous spectrum white light pulses generated by converging ultra-short optical pulses on a point in a transparent substance 23 are dispersed into a spectrum of light by a dispersing element 25, and a plurality of objective wavelength components are separately selected by small reflection mirrors 37, masked mirrors or the like. The selected objective wavelength components are combined by the dispersing element 25 into a composite optical pulse containing the objective wavelength components. The composite optical pulse is irradiated onto the bio-sample 28 or other samples, whereby only the desired fluorescent substances are excited by the objective wavelength components corresponding to them. The selected objective wavelength components may preferably have different light path lengths. By this construction, the component optical pulses corresponding to the objective wavelengths in the composite optical pulse are separated from each other, so that the noise due to the interference of different wavelength components is prevented.

摘要翻译： 本发明提出了一种用于分析样品（特别是生物样品）的方法和系统，其中以简单的方式使用从超短光脉冲产生的连续光谱白光脉冲。 通过根据本发明的系统，通过在透明物质23中的点聚焦超短光脉冲而产生的连续光谱白光脉冲通过分散元件25分散到光谱中，并且多个目标波长分量是 通过小反射镜37分别选择掩模镜等。 所选择的目标波长分量由分散元件25组合成包含目标波长分量的复合光脉冲。 将复合光脉冲照射到生物样品28或其他样品上，由此只有所需的荧光物质被与它们相对应的目标波长成分激发。 所选择的目标波长分量可以优选地具有不同的光路长度。 通过这种结构，与复合光脉冲中的目标波长相对应的分量光脉冲彼此分离，从而防止了由于不同波长分量的干扰引起的噪声。