公开(公告)号：US20180066023A1

公开(公告)日：2018-03-08

申请号：US15817761

申请日：2017-11-20

Applicant: The Catholic University of America

Inventor： Venigalla B. RAO ,  Wadad ALSALMI

IPC: C07K14/005 ,  A61K9/70 ,  C12N7/00

CPC classification number: C07K14/005 ,  A61K9/7023 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/54 ,  A61K2039/627 ,  C07K1/22 ,  C07K1/36 ,  C07K14/162 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/40 ,  C07K2319/50 ,  C12N7/00 ,  C12N2740/16022 ,  C12N2740/16043 ,  C12N2740/16051 ,  C12N2740/16111 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16234

Abstract: An approach of producing recombinant trimers that mimic native HIV-1 envelope trimers is developed. A recombinant protein forming the recombinant trimers encompasses a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140. The linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix. After expressed in a cell, the recombinant protein is secreted into the culture medium and assembles into recombinant trimers therein. The recombinant trimers may be directly purified from the culture medium. Cleaved and uncleaved trimers from different clade viruses are produced.

公开(公告)号：US20160272947A1

公开(公告)日：2016-09-22

申请号：US14806739

申请日：2015-07-23

Applicant: The Catholic University of America

Inventor： Venigalla B. RAO ,  Wadad ALSALMI

IPC: C12N7/00 ,  C07K14/005

CPC classification number: C07K14/005 ,  A61K9/7023 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/54 ,  A61K2039/627 ,  C07K1/22 ,  C07K1/36 ,  C07K14/162 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/40 ,  C07K2319/50 ,  C12N7/00 ,  C12N2740/16022 ,  C12N2740/16043 ,  C12N2740/16051 ,  C12N2740/16111 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16234

Abstract: An approach of producing recombinant trimers that mimic native HIV-1 envelope trimers is developed. A recombinant protein forming the recombinant trimers encompasses a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140. The linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix. After expressed in a cell, the recombinant protein is secreted into the culture medium and assembles into recombinant trimers therein. The recombinant trimers may be directly purified from the culture medium. Cleaved and uncleaved trimers from different clade viruses are produced.

公开(公告)号：US20160271243A1

公开(公告)日：2016-09-22

申请号：US14806751

申请日：2015-07-23

Applicant: THE CATHOLIC UNIVERSITY OF AMERICA

Inventor： Venigalla B. RAO ,  Wadad ALSALMI

IPC: A61K39/21 ,  A61K9/70

CPC classification number: C07K14/005 ,  A61K9/7023 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/54 ,  A61K2039/627 ,  C07K1/22 ,  C07K1/36 ,  C07K14/162 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/40 ,  C07K2319/50 ,  C12N7/00 ,  C12N2740/16022 ,  C12N2740/16043 ,  C12N2740/16051 ,  C12N2740/16111 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16234

Abstract: Provided herein are HIV vaccines that encompasses recombinant trimers that mimic native HIV-1 envelope trimers. Also provided are methods of administering to a subject in need thereof an HIV vaccine provided herein to elicit antibodies against a recombinant trimer in the subject. A recombinant trimer is formed by a recombinant protein comprising a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140, wherein the linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix during purification of the recombinant trimer.

Abstract translation: 本文提供涵盖模仿天然HIV-1包膜三聚体的重组三聚体的HIV疫苗。 还提供了向有需要的受试者施用本文提供的HIV疫苗以引发针对受试者中重组三聚体的抗体的方法。 通过重组蛋白质形成重组三聚体，所述重组蛋白质包含通过重组HIV-1 gp140的C-末端的接头与标签融合的重组HIV-1 gp140，其中所述连接体足够长，使得所述标签可通过 在纯化重组三聚体期间结合在固体基质上的结合分子。

公开(公告)号：US20220184205A1

公开(公告)日：2022-06-16

申请号：US17548629

申请日：2021-12-13

Applicant: The Catholic University of America

Inventor： Venigalla B. RAO ,  Jingen ZHU

IPC: A61K39/215 ,  C12N7/00 ,  C12N15/86 ,  C12N15/90

Abstract: The present disclosure relates to a system for and a method of incorporating SARS-CoV-2 genes and proteins into T4 phages. The present disclosure also relates to vaccine against SARS-CoV-2 containing recombinant T4 phages created using the method provided in the present disclosure.

公开(公告)号：US20220090139A1

公开(公告)日：2022-03-24

申请号：US17546183

申请日：2021-12-09

Applicant: The Catholic University of America

Inventor： Venigalla B. RAO ,  Jingen ZHU

IPC: C12N15/86 ,  C12N7/00

Abstract: Described is an “artificial virus” (AV) programmed with biomolecules that can enter human cells and carry out precise human genome modification. The AVs comprise: at least one viral vector, such as bacteriophage T4; at least one therapeutic molecule, such as DNA, RNA, protein and their complex; and a lipid coating. Also described is a method of human genome modification, using such an AV, and a method of program such an AV.

公开(公告)号：US20180194811A1

公开(公告)日：2018-07-12

申请号：US15908372

申请日：2018-02-28

Applicant: The Catholic University of America

Inventor： Venigalla B. RAO ,  Wadad ALSALMI

IPC: C07K14/005 ,  A61K9/70 ,  C12N7/00

CPC classification number: C07K14/005 ,  A61K9/7023 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/54 ,  A61K2039/627 ,  C07K1/22 ,  C07K1/36 ,  C07K14/162 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/40 ,  C07K2319/50 ,  C12N7/00 ,  C12N2740/16022 ,  C12N2740/16043 ,  C12N2740/16051 ,  C12N2740/16111 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16234

Abstract: Provided herein are HIV vaccines that encompasses recombinant trimers that mimic native HIV-1 envelope trimers. Also provided are methods of administering to a subject in need thereof an HIV vaccine provided herein to elicit antibodies against a recombinant trimer in the subject. A recombinant trimer is formed by a recombinant protein comprising a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140, wherein the linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix during purification of the recombinant trimer.

公开(公告)号：US20160272685A1

公开(公告)日：2016-09-22

申请号：US14806735

申请日：2015-07-23

Applicant: THE CATHOLIC UNIVERSITY OF AMERICA

Inventor： Venigalla B. RAO ,  Wadad ALSALMI

IPC: C07K14/005 ,  C07K1/36 ,  C12N7/00 ,  C07K1/22

CPC classification number: C07K14/005 ,  A61K9/7023 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/54 ,  A61K2039/627 ,  C07K1/22 ,  C07K1/36 ,  C07K14/162 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/40 ,  C07K2319/50 ,  C12N7/00 ,  C12N2740/16022 ,  C12N2740/16043 ,  C12N2740/16051 ,  C12N2740/16111 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16234

Abstract: An approach of producing recombinant trimers that mimic native HIV-1 envelope trimers is developed. A recombinant protein forming the recombinant trimers encompasses a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140. The linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix. After expressed in a cell, the recombinant protein is secreted into the culture medium and assembles into recombinant trimers therein. The recombinant trimers may be directly purified from the culture medium. Cleaved and uncleaved trimers from different clade viruses are produced.

公开(公告)号：US20160272684A1

公开(公告)日：2016-09-22

申请号：US14806727

申请日：2015-07-23

Applicant: The Catholic University of America

Inventor： Venigalla B. RAO ,  Wadad ALSALMI

IPC: C07K14/005 ,  C12N7/00

CPC classification number: C07K14/005 ,  A61K9/7023 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/54 ,  A61K2039/627 ,  C07K1/22 ,  C07K1/36 ,  C07K14/162 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/40 ,  C07K2319/50 ,  C12N7/00 ,  C12N2740/16022 ,  C12N2740/16043 ,  C12N2740/16051 ,  C12N2740/16111 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16234

Abstract: An approach of producing recombinant trimers that mimic native HIV-1 envelope trimers is developed. A recombinant protein forming the recombinant trimers encompasses a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140. The linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix. After expressed in a cell, the recombinant protein is secreted into the culture medium and assembles into recombinant trimers therein. The recombinant trimers may be directly purified from the culture medium. Cleaved and uncleaved trimers from different clade viruses are produced.

公开(公告)号：US20160207964A1

公开(公告)日：2016-07-21

申请号：US15080804

申请日：2016-03-25

Applicant: The Catholic University of America

Inventor： Venigalla B. RAO ,  Guofen GAO

IPC: C07K14/005 ,  C12N7/00

CPC classification number: C07K14/005 ,  C07K2319/35 ,  C07K2319/70 ,  C07K2319/735 ,  C12N7/00 ,  C12N7/02 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2795/10122 ,  C12N2795/10123

Abstract: Described herein is a soluble HIV-1 retrovirus transmembrane glycoprotein gp41 trimer (Soc-gp41M-Fd) containing a partial ectodomain and the cytoplasmic domain, that is fused to the small outer capsid (Soc) protein of bacteriophage T4 and the Foldon domain of the bacteriophage T4 fibritin (Fd). The gp41 trimer that has a prehairpin structure could be utilized to understand the mechanism of viral entry and as a candidate for development of HIV-1 vaccines, diagnostics and therapeutics. Other secondary embodiments of the gp41 proteins containing different modifications are also disclosed. According to one embodiment, the gp41 trimer is further attached to a cell penetration peptide (CPP). Methods of producing gp41 trimers are also disclosed.

Abstract translation: 本文描述的是含有部分胞外域和细胞质结构域的可溶性HIV-1逆转录病毒跨膜糖蛋白gp41三聚体（Soc-gp41M-Fd），其与噬菌体T4的小外壳（Soc）蛋白和 噬菌体T4纤维蛋白（Fd）。 具有前贴片结构的gp41三聚体可用于了解病毒进入的机制，并且作为HIV-1疫苗，诊断和治疗学的发展的候选者。 还公开了含有不同修饰物的gp41蛋白质的其它次要实施方案。 根据一个实施方案，gp41三聚体进一步连接于细胞穿透肽（CPP）。 还公开了生产gp41三聚体的方法。