公开(公告)号：US11441137B2

公开(公告)日：2022-09-13

申请号：US16884766

申请日：2020-05-27

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12Q1/6809 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20200299660A1

公开(公告)日：2020-09-24

申请号：US16896711

申请日：2020-06-09

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20200017879A1

公开(公告)日：2020-01-16

申请号：US16335516

申请日：2017-09-28

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Jillian F. Banfield ,  David Burstein ,  Lucas Benjamin Harrington ,  Steven C. Strutt

IPC: C12N15/85 ,  C12N15/10 ,  C12N15/52 ,  C12N15/113 ,  C12N15/74 ,  C12N15/86 ,  C12N15/88 ,  C12N15/90 ,  C12N9/10 ,  C12N9/22

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

公开(公告)号：US20180346927A1

公开(公告)日：2018-12-06

申请号：US16054513

申请日：2018-08-03

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Jillian Banfield ,  David Burstein ,  Lucas Benjamin Harrington ,  Steven C. Strutt

IPC: C12N15/85 ,  C12N9/22 ,  C12N15/88 ,  C12N15/113 ,  C12N15/74 ,  C12N15/62 ,  C12N15/52 ,  C12N9/10 ,  C07K19/00

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

公开(公告)号：US12258575B2

公开(公告)日：2025-03-25

申请号：US17749017

申请日：2022-05-19

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Jillian F. Banfield ,  David Burstein ,  Lucas Benjamin Harrington

IPC: C12N9/22 ,  A61K38/46 ,  C12N15/113 ,  C12N15/90

Abstract: The present disclosure provides CasY proteins, nucleic acids encoding the CasY proteins, and modified host cells comprising the CasY proteins and/or nucleic acids encoding same. CasY proteins are useful in a variety of applications, which are provided. The present disclosure provides CasY guide RNAs that bind to and provide sequence specificity to the CasY proteins, nucleic acids encoding the CasY guide RNAs, and modified host cells comprising the CasY guide RNAs and/or nucleic acids encoding same. CasY guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides methods of identifying a CRISPR RNA-guided endonuclease.

公开(公告)号：US20240167052A1

公开(公告)日：2024-05-23

申请号：US18513347

申请日：2023-11-17

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Jillian F. Banfield ,  David Burstein ,  Lucas Benjamin Harrington ,  Steven C. Strutt

IPC: C12N15/85 ,  C07K19/00 ,  C12N9/10 ,  C12N9/22 ,  C12N15/10 ,  C12N15/113 ,  C12N15/52 ,  C12N15/62 ,  C12N15/74 ,  C12N15/86 ,  C12N15/88 ,  C12N15/90

CPC classification number: C12N15/8509 ,  C07K19/00 ,  C12N9/1007 ,  C12N9/1025 ,  C12N9/22 ,  C12N15/102 ,  C12N15/113 ,  C12N15/52 ,  C12N15/62 ,  C12N15/74 ,  C12N15/85 ,  C12N15/86 ,  C12N15/88 ,  C12N15/902 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/06 ,  C07K2319/09 ,  C07K2319/20 ,  C12N2310/20 ,  C12N2740/13043 ,  C12N2740/16043 ,  C12N2750/14143

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

公开(公告)号：US20240167009A1

公开(公告)日：2024-05-23

申请号：US18393303

申请日：2023-12-21

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C07K2319/09 ,  C12N2310/20 ,  C12N2800/80

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US11970719B2

公开(公告)日：2024-04-30

申请号：US16755535

申请日：2018-10-31

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/82

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/8213 ,  C12N2310/20

Abstract: Provided are compositions and methods that include one or more of: (1) a Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the effector protein, and/or a modified host cell comprising the effector protein (and/or a nucleic acid encoding the same); (2) a CRISPR/Cas guide RNA that binds to and provides sequence specificity to the Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the CRISPR/Cas guide RNA, and/or a modified host cell comprising the CRISPR/Cas guide RNA (and/or a nucleic acid encoding the same); and (3) a CRISPR/Cas transactivating noncoding RNA (trancRNA), a nucleic acid encoding the CRISPR/Cas trancRNA, and/or a modified host cell comprising the CRISPR/Cas trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20230323319A1

公开(公告)日：2023-10-12

申请号：US17935521

申请日：2022-09-26

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N2310/20 ,  C12N2800/80 ,  C07K2319/09

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US11453866B2

公开(公告)日：2022-09-27

申请号：US16896711

申请日：2020-06-09

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).