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    Enterobacteriaceae fermentation strains
    13.
    发明申请
    Enterobacteriaceae fermentation strains 审中-公开
    肠杆菌科发酵菌株

    公开(公告)号：US20070105203A1

    公开(公告)日：2007-05-10

    申请号：US11652353

    申请日：2007-01-11

    Applicant: Timothy Fowler ,  Stuart Causey

    Inventor： Timothy Fowler ,  Stuart Causey

    IPC: C12P7/58 ,  C12N15/74 ,  C12N1/21

    CPC classification number: C07K14/24 ,  C12N15/74 ,  C12P7/60

    Abstract: Methods are provided for preparing improved fermentation strains of the family Enterobacteriaceae which comprise the steps of eliminating the cryptic plasmid from the progenitor strain thereby creating the improved strain. Methods for reducing the mobilization properties of resident plasmids in an Enterobacteriaceae strain containing a cryptic plasmid are also provided. The present invention provides the nucleic acid sequence of pS, a cryptic plasmid found in Pantoea which can be used to identify the cryptic plasmid in strains of Enterobacteriaceae.

    Abstract translation: 提供了用于制备肠杆菌科的改良发酵菌株的方法，其包括从祖细胞菌株中消除神经质粒的步骤，从而产生改良的菌株。 还提供了用于降低含有隐性质粒的肠杆菌科菌株中驻留质粒的动员特性的方法。 本发明提供了在Pantoea中发现的可用于鉴定肠杆菌科菌株中的隐性质粒的隐神质的pS的核酸序列。

    Enzyme feed additive and animal feed including it
    15.
    发明授权
    Enzyme feed additive and animal feed including it 失效
    酶饲料添加剂和动物饲料包括它

    公开(公告)号：US07005128B1

    公开(公告)日：2006-02-28

    申请号：US10379978

    申请日：2003-03-04

    Applicant: Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

    Inventor： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

    IPC: A61K38/47 ,  A61K38/43 ,  A61K38/54 ,  A23K1/00

    CPC classification number: C12Y302/01004 ,  A23K20/189 ,  A23K50/75 ,  C12N9/2437 ,  C12Y302/01091

    Abstract: The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0–20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII, EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0–20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0–20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. Such enzyme-based feed additives can be incorporated into a cereal-based feed which includes one or more of barley, wheat, triticale, rye and maize. The feed additive has the advantage of improving the feed conversion ratio and/or increasing the digestibility of a cereal-based feed in which it is included.

    Abstract translation: 提供的组合物作为饲料添加剂，其包含一种或多种内切葡聚糖酶，并且基于组合物中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 所述凝集素可以是EGI，EGII，EGIII及其任何的任何功能活性衍生物中的一种或多种。 这样的内切葡聚糖酶可以从真菌木霉的遗传修饰菌株获得。 还提供了一种基于酶的饲料添加剂，其包含缺乏纤维素结合结构域的EGI和/或EGII，并且基于添加剂中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 提供了另外一种基于酶的饲料添加剂，其包含基于谷物的载体，一种或多种内切葡聚糖酶，和基于添加剂中的纤维素酶蛋白质含量为0-20重量％的纤维二糖水解酶。 这种基于酶的饲料添加剂可以掺入包括大麦，小麦，黑小麦，黑麦和玉米中的一种或多种的谷类饲料中。 饲料添加剂具有提高饲料转化率和/或增加其中包含谷物饲料的消化率的优点。

    Enterobacteriaceae fermentation strains
    16.
    发明申请
    Enterobacteriaceae fermentation strains 审中-公开

    公开(公告)号：US20050208662A1

    公开(公告)日：2005-09-22

    申请号：US11132997

    申请日：2005-05-19

    Applicant: Timothy Fowler ,  Stuart Causey

    Inventor： Timothy Fowler ,  Stuart Causey

    IPC: C07K14/24 ,  C12N15/31 ,  C12N15/74 ,  C12P7/60 ,  C12N1/21

    CPC classification number: C07K14/24 ,  C12N15/74 ,  C12P7/60

    Abstract: Methods are provided for preparing improved fermentation strains of the family Enterobacteriaceae which comprise the steps of eliminating the cryptic plasmid from the progenitor strain thereby creating the improved strain. Methods for reducing the mobilization properties of resident plasmids in an Enterobacteriaceae strain containing a cryptic plasmid are also provided. The present invention provides the nucleic acid sequence of pS, a cryptic plasmid found in Pantoea which can be used to identify the cryptic plasmid in strains of Enterobacteriaceae.

    Enzyme feed additive and animal feed including it
    17.
    发明授权
    Enzyme feed additive and animal feed including it 失效
    酶饲料添加剂和动物饲料包括它

    公开(公告)号：US06562340B1

    公开(公告)日：2003-05-13

    申请号：US08507362

    申请日：1995-08-16

    Applicant: Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

    Inventor： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

    IPC: A61K3847

    CPC classification number: C12Y302/01004 ,  A23K20/189 ,  C12N9/2437 ,  C12N15/80 ,  C12Y302/01021 ,  C12Y302/01091

    Abstract: The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. Such enzyme-based feed additives can be incorporated into a cereal-based feed which includes one or more of barley, wheat, tricale, rye and maize. The feed additive has the advantage of improving the feed conversion ratio and/or increasing the digestibility of a cereal-based feed in which it is included.

    Abstract translation: 提供的组合物作为饲料添加剂，其包含一种或多种内切葡聚糖酶，并且基于组合物中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 所述凝集素可以是一种或多种EGI，EGII EGIII及其任何功能活性的衍生物。 这样的内切葡聚糖酶可以从真菌木霉的遗传修饰菌株获得。 还提供了一种基于酶的饲料添加剂，其包含缺乏纤维素结合结构域的EGI和/或EGII，并且基于添加剂中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 提供了另外一种基于酶的饲料添加剂，其包含基于谷物的载体，一种或多种内切葡聚糖酶，和基于添加剂中的纤维素酶蛋白质含量为0-20重量％的纤维二糖水解酶。 这种基于酶的饲料添加剂可以掺入包括一种或多种大麦，小麦，三倍体，黑麦和玉米的谷类饲料中。 饲料添加剂具有提高饲料转化率和/或增加其中包含谷物饲料的消化率的优点。

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