公开(公告)号：US20050039225A1

公开(公告)日：2005-02-17

申请号：US10641100

申请日：2003-08-15

申请人： Moon-Soo Soh ,  Pill-Soon Song ,  Ki-Young Yang

发明人： Moon-Soo Soh ,  Pill-Soon Song ,  Ki-Young Yang

IPC分类号： C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/02

CPC分类号： C12N15/8237

摘要： This invention is about the functionally hyperactive light signal related molecule, HFR1-ΔN105, of which the nucleic acids that encode N-terminal 105 amino acid residues were deleted. HFR1 as a bHLH transcription factor functions in a subset of phytochrome A signaling cascade and it was reported to be regulated negatively by COP1. Experiments with a HFR1-ΔN105 overexpressing plant revealed that the deletion of N-terminal amino acids makes the HFR1 more active in photomorphogenic development such as germination and de-etiolation. In addition, the transgenic plants showed hypersensitive photo-responses in the inhibition of hypocotyl elongation, dependently on another positive element of light signaling, a bZIP protein, HY5. The end-of-day far-red light response and petiole elongation were suppressed in the HFR1-ΔN105 overexpressing plants. These results suggest that N-terminal region of HFR1 negatively regulate HFR1 function and that HFR1-ΔN105 is hyperactive.

摘要翻译： 本发明涉及功能性超活动光信号相关分子HFR1-DeltaN105，其中编码N末端105个氨基酸残基的核酸被缺失。 HFR1作为bHLH转录因子在植物色素A信号级联的一个子集中起作用，据报道COP1负调节。 HFR1-DeltaN105过表达植物的实验显示，N末端氨基酸的缺失使得HFR1在光形态发生如萌发和脱乙酰化中更具活性。 此外，转基因植物在抑制下胚轴延伸方面表现出过敏光响应，依赖于光信号传导的另一阳性元件bZIP蛋白HY5。 在HFR1-DeltaN105过表达植物中，终末的远红光反应和叶柄伸长被抑制。 这些结果表明，HFR1的N末端区域负调节HFR1功能，HFR1-DeltaN105是多活性的。

公开(公告)号：US06518484B2

公开(公告)日：2003-02-11

申请号：US09815622

申请日：2001-03-23

申请人： Jeong-Gu Kang ,  Ju Yun ,  Kyung-Sook Chung ,  Pill-Soon Song ,  Chung-Mo Park

发明人： Jeong-Gu Kang ,  Ju Yun ,  Kyung-Sook Chung ,  Pill-Soon Song ,  Chung-Mo Park

IPC分类号： A01H100

CPC分类号： C07K14/415 ,  C12N15/8216

摘要： The present invention provides a nucleic acid molecule isolated from the 5′ untranscribed region of a translationally controlled tumor protein (TCTP) gene from Arabidopsis. The nucleic acid molecule, referred to as TCTP promoter in the present invention, directs the high-level expression of a reporter gene that is properly placed under its control. The TCTP promoter initiates and regulates the gene expression to a level that is comparable to those by the currently available promoters in plant genetic engineering. A minimum of 300-bp region is enough for fill regulatory activity. The TCTP promoter is functional in all plant tissues tested with the highest activity in the meristem tissue of root tip. The present invention can be utilized to express useful genes to a high level in agronomically important plants.

公开(公告)号：US06284952B1

公开(公告)日：2001-09-04

申请号：US09239909

申请日：1999-01-29

申请人： Won Do Heo ,  Moo Je Cho ,  Pill-Soon Song ,  Chang Ho Chung

发明人： Won Do Heo ,  Moo Je Cho ,  Pill-Soon Song ,  Chang Ho Chung

IPC分类号： A01H500

CPC分类号： C12N15/8283 ,  C07K14/415 ,  C12N15/8279

摘要： The present invention provides transgenic plants and plant cells thereof which have been transformed with the soybean calmodulin isoform (SCaM5) gene to exhibit greatly enhanced resistance to a wide spectrum of plant pathogens. The present invention also provides the expression vector containing SCaM5 gene and to host cells into which the gene in the expression vector has been introduced to plant pathogens-resistant plants. Transgenic plants expressing a heterologous SCaM5 gene show increased resistance to fungi, bacteria and viruses which normally infect the plants.

摘要翻译： 本发明提供已经用大豆钙调蛋白同种型（SCaM5）基因转化的转基因植物及其植物细胞，以显示出对广谱植物病原体的极大增强的抗性。 本发明还提供含有SCaM5基因的表达载体和宿主细胞，其中表达载体中的基因已被引入植物病原体抗性植物中。 表达异源SCaM5基因的转基因植物对通常感染植物的真菌，细菌和病毒的抗性增加。

公开(公告)号：US20060037100A1

公开(公告)日：2006-02-16

申请号：US10916419

申请日：2004-08-12

申请人： Young Kim ,  Moon Ko ,  Hyo Seo ,  Jung Cho ,  Pill-Soon Song

发明人： Young Kim ,  Moon Ko ,  Hyo Seo ,  Jung Cho ,  Pill-Soon Song

IPC分类号： C12N15/82 ,  A01H1/00 ,  C12N15/87

CPC分类号： C12N9/16 ,  C07K14/415 ,  C12N15/8282

摘要： A stable pepper transformation was established using Agrobacterium mediated method. Pepper plants were transformed with PepEST or PepDef gene, where the expression of the nucleic acid sequence in the plant resulted in increased resistance to fungal infection as compared to the wild type plant. Provided are agricultural products including seeds produced by the transgenic plants. Also provided are vectors and host cells containing the nucleic acids coding PepEST and PepDef, respectively.

摘要翻译： 使用农杆菌介导的方法建立稳定的胡椒转化。 用PepEST或PepDef基因转化辣椒植物，与野生型植物相比，植物中核酸序列的表达导致对真菌感染的抗性增加。 提供农产品，包括由转基因植物生产的种子。 还提供了分别含有编码PepEST和PepDef的核酸的载体和宿主细胞。

公开(公告)号：US06998120B2

公开(公告)日：2006-02-14

申请号：US10848375

申请日：2004-05-19

申请人： Young Soon Kim ,  Hyo Hyoun Seo ,  Moon Kyung Ko ,  Boung-Jun Oh ,  Pill-Soon Song

发明人： Young Soon Kim ,  Hyo Hyoun Seo ,  Moon Kyung Ko ,  Boung-Jun Oh ,  Pill-Soon Song

IPC分类号： A61K38/46 ,  C12N9/16 ,  C12N9/20 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12Y301/01 ,  A01N37/18 ,  A01N37/46 ,  A01N65/08 ,  A61K38/00 ,  C12N9/18 ,  C12N15/8282

摘要： This invention relates to dual roles of the pepper esterase protein as a biocontrol agent that can elicit defense reactions in plant cells and as direct fungal inhibitor that can block fungal penetration into plant cells. The possible enzymatic actions of the PepEST protein were elucidated in both fungus and plant to gain insight into the molecular mechanisms involved in the fungal resistance. Exogenously treated PepEST protein on the unripe pepper fruits decomposed the cuticles of the fruits and released glycerol and 9-octadecenamide. The treatment resulted in the massive generation of hydrogen peroxide in the unripe pepper fruit and simultaneously elicited the expression of defense-related genes in both absence and presence of the fungus.

摘要翻译： 本发明涉及胡椒酯酶蛋白作为可在植物细胞中引发防御反应的生物防治剂的双重作用，以及可阻断真菌渗入植物细胞的直接真菌抑制剂。 在真菌和植物中阐明了PepEST蛋白的可能的酶作用，以了解参与真菌抗性的分子机制。 未成熟胡椒果实外源性处理的PepEST蛋白分解果实的角质层，释放甘油和9-十八碳烯酰胺。 该处理导致未成熟胡椒果实中大量生成过氧化氢，并且在真菌存在和存在的同时引发防御相关基因的表达。

公开(公告)号：US20050260184A1

公开(公告)日：2005-11-24

申请号：US10848375

申请日：2004-05-19

申请人： Young Kim ,  Hyo Seo ,  Moon Ko ,  Boung-Jun Oh ,  Pill-Soon Song

发明人： Young Kim ,  Hyo Seo ,  Moon Ko ,  Boung-Jun Oh ,  Pill-Soon Song

IPC分类号： A01N37/18 ,  A01N37/46 ,  A61K38/46 ,  C12N9/16 ,  C12N9/18 ,  C12N15/82

CPC分类号： C12Y301/01 ,  A01N37/18 ,  A01N37/46 ,  A01N65/08 ,  A61K38/00 ,  C12N9/18 ,  C12N15/8282

摘要： This invention relates to dual roles of the pepper esterase protein as a biocontrol agent that can elicit defense reactions in plant cells and as direct fungal inhibitor that can block fungal penetration into plant cells. The possible enzymatic actions of the PepEST protein were elucidated in both fungus and plant to gain insight into the molecular mechanisms involved in the fungal resistance. Exogenously treated PepEST protein on the unripe pepper fruits decomposed the cuticles of the fruits and released glycerol and 9-octadecenamide. The treatment resulted in the massive generation of hydrogen peroxide in the unripe pepper fruit and simultaneously elicited the expression of defense-related genes in both absence and presence of the fungus.

摘要翻译： 本发明涉及胡椒酯酶蛋白作为可在植物细胞中引发防御反应的生物防治剂的双重作用，以及可阻断真菌渗入植物细胞的直接真菌抑制剂。 在真菌和植物中阐明了PepEST蛋白的可能的酶作用，以了解参与真菌抗性的分子机制。 未成熟胡椒果实外源性处理的PepEST蛋白分解果实的角质层，释放甘油和9-十八碳烯酰胺。 该处理导致未成熟胡椒果实中大量生成过氧化氢，并且在真菌存在和存在的同时引发防御相关基因的表达。

公开(公告)号：US06586252B2

公开(公告)日：2003-07-01

申请号：US09916338

申请日：2001-07-30

申请人： Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park

发明人： Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park

IPC分类号： C12N1529

CPC分类号： C12N9/16 ,  C12N15/827

摘要： This invention relates to a nucleic acid molecule encoding the catalytic subunit of a protein phosphatase (PP2AC-JD) that belongs to the PP2A family. The PP2AC-JD interacts with the phytochrome A, a primary photoreceptor in the light signal transduction in plants, in the photoperiodic control of flowering. The present invention also provides the methods and processes for generating transgenic higher plants transformed with said nucleic acid molecule to engineer flowing time of economically important crop plants.

摘要翻译： 本发明涉及编码属于PP2A家族的蛋白质磷酸酶（PP2AC-JD）的催化亚基的核酸分子。 PP2AC-JD与植物色素A（植物光信号转导中的初级感光体）在开花的光周期控制中相互作用。 本发明还提供用于产生用所述核酸分子转化的转基因高等植物以设计经济上重要的作物植物的流动时间的方法和方法。

公开(公告)号：US06545202B2

公开(公告)日：2003-04-08

申请号：US09737300

申请日：2000-12-15

申请人： Jeong-Gu Kang ,  Ju Yun ,  Pill-Soon Song ,  Chung-Mo Park

发明人： Jeong-Gu Kang ,  Ju Yun ,  Pill-Soon Song ,  Chung-Mo Park

IPC分类号： A01H1100

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： The present invention provides transgenic higher plants and transgenic plant cells thereof which have been transformed with a plant translationally controlled tumor protein (TCTP) gene to improve growth rate in a growth stage-dependent manner, especially in the earlier vegetative growth stage from seedlings to young plants. Also, the invention provides methods for engineering higher plants so that they can be more efficiently transformed with other transforming vector constructs. Further, provided are TCTP expression vectors which contain an uninterrupted coding region for the TCTP protein used to generate transgenic plants and cells thereof.