公开(公告)号：US06175692B1

公开(公告)日：2001-01-16

申请号：US09317944

申请日：1999-05-25

申请人： Hitoshi Onoda ,  Terutake Kadohara ,  Tomokazu Yoshida

发明人： Hitoshi Onoda ,  Terutake Kadohara ,  Tomokazu Yoshida

IPC分类号： G03B1336

CPC分类号： G03B13/16 ,  G02B7/36 ,  G02B27/28 ,  G03B13/36

摘要： A focus detecting device capable of focus detection in a plurality of focus-detecting areas, includes a setter for setting a first group including at least two focus-detecting areas, and a second group including a plurality of focus-detecting areas, which are not included in the first group, and a focus detector means for executing focus detection in the order from the focus-detecting areas included in the first group. The focus detecter ends focus detection without executing focus detection in the focus-detecting areas included in the second group, when the focus-detecting area included in the first group satisfies a predetermined condition.

摘要翻译： 一种能够在多个聚焦检测区域进行聚焦检测的聚焦检测装置，包括用于设置包括至少两个焦点检测区域的第一组的设定器和不包括多个焦点检测区域的第二组 包括在第一组中的焦点检测装置和用于从包括在第一组中的对焦检测区域起的顺序执行焦点检测的焦点检测器装置。 当第一组中包括的对焦检测区域满足预定条件时，焦点检测器在聚焦检测区域中不执行焦点检测，结束焦点检测。

公开(公告)号：US20110095166A1

公开(公告)日：2011-04-28

申请号：US12907278

申请日：2010-10-19

申请人： Tomokazu Yoshida

发明人： Tomokazu Yoshida

IPC分类号： G02B7/04

CPC分类号： G02B7/28

摘要： An auto focus adjustment apparatus includes: a correction unit which corrects a defocus amount detected by a defocus amount detection unit, correspondingly to a focus area selected from a plurality of focus areas; a storage unit which stores a correction value to be used by the correction unit at a time of correcting the defocus amount; and a correction value changing unit configured to change the correction value, wherein the correction value changing unit is arranged to be capable of changing the correction value for a plurality of selected focus areas and at a plurality of zoom positions.

摘要翻译： 自动对焦调整装置包括：校正单元，其对应于从多个焦点区域选择的对焦区域来校正由散焦量检测单元检测到的散焦量; 存储单元，其存储在校正所述散焦量时由所述校正单元使用的校正值; 以及校正值改变单元，被配置为改变校正值，其中校正值改变单元被布置为能够改变多个选择的聚焦区域和多个变焦位置处的校正值。

公开(公告)号：US07853138B2

公开(公告)日：2010-12-14

申请号：US12028231

申请日：2008-02-08

申请人： Tomokazu Yoshida

发明人： Tomokazu Yoshida

IPC分类号： G03B13/22 ,  G03B13/20

CPC分类号： G03B7/095

摘要： A camera is provided which is adapted to receive a photographic lens having a diaphragm. The camera includes a detection unit configured to execute electric charge accumulation for detecting a defocus amount when the diaphragm is in a state having a first aperture value, and a controller configured to, when causing the diaphragm to perform an aperture reducing operation from the first aperture value to a second aperture value, cause the diaphragm to start the aperture reducing operation without waiting for completion of the electric charge accumulation for detecting the defocus amount by the detection unit.

摘要翻译： 提供了适于接收具有隔膜的摄影镜头的照相机。 摄像机包括：检测单元，被配置为执行电荷累积，用于当所述隔膜处于具有第一孔径值的状态时检测散焦量;以及控制器，被配置为当使所述隔膜从所述第一孔径执行孔径减小操作时 值到第二光圈值，使得光阑开始光圈减小操作，而不用等待完成由检测单元检测散焦量的电荷累积。

公开(公告)号：US07842454B2

公开(公告)日：2010-11-30

申请号：US11802830

申请日：2007-05-25

申请人： Keiichiro Shomi ,  Tomokazu Yoshida

发明人： Keiichiro Shomi ,  Tomokazu Yoshida

IPC分类号： B65D39/00 ,  C12Q1/70

CPC分类号： B01L3/502707 ,  B01L3/502 ,  B01L3/5082 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/0672 ,  B01L2300/0822 ,  B01L2400/0406 ,  B01L2400/0683

摘要： A kit for preparing a cancer cell detection sample which is highly safe, simple, and high in precision, and can detect a cancer cell quantitatively, and a kit for cancer cell detection using the same, as well as a method for diagnosing cancer using those kits, and a method for preparing a sample for cancer cell detection are provided. A kit for preparing a detection sample for detecting a cancer cell of the present invention, comprising a test container having an opening for receiving a biological sample collected from a subject, and a reagent inclusion part for accommodating a reagent containing a virus, a seal part for sealing the reagent inclusion part of the test container, a cap for closing the opening, and an opener for breaking the seal part.

摘要翻译： 一种用于制备高度安全，简单且高精度的癌细胞检测样品的试剂盒，并且可以定量检测癌细胞，以及使用该试剂盒进行癌细胞检测的试剂盒，以及使用这些试剂诊断癌症的方法 提供了用于癌细胞检测的样品的制备方法。 一种制备用于检测本发明的癌细胞的检测样品的试剂盒，包括具有用于接收从受试者收集的生物样品的开口的测试容器和用于容纳含有病毒的试剂的试剂包含部分，密封部分 用于密封测试容器的试剂包含部分，用于封闭开口的盖和用于破坏密封部的开启器。

公开(公告)号：US07718393B2

公开(公告)日：2010-05-18

申请号：US11939138

申请日：2007-11-13

申请人： Amane Harada ,  Tomokazu Yoshida

发明人： Amane Harada ,  Tomokazu Yoshida

IPC分类号： C12Q1/02

CPC分类号： G01N33/57415 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/158 ,  G01N2333/9121 ,  G01N2800/44

摘要： The present invention provides a method for determining susceptibility to an anthracycline anticancer agent, comprising the steps consisting of detecting the expression of activated Akt in a cancer cell or cancer tissue and determining the susceptibility of the cancer cell or cancer tissue to the anthracycline anticancer agent on the basis of the detection result. The method for determining the susceptibility to an anticancer agent can be used in objective and accurate determination of the susceptibility of a cancer cell or cancer tissue to an anthracycline anticancer agent without imposing a heavy burden on a patient.

摘要翻译： 本发明提供一种测定对蒽环类抗癌药敏感性的方法，包括以下步骤：检测癌细胞或癌组织中活化的Akt的表达，并测定癌细胞或癌组织对蒽环类抗癌剂的敏感性 检测结果的基础。 用于确定对抗癌剂易感性的方法可用于客观和准确地确定癌细胞或癌组织对蒽环类抗癌剂的敏感性，而不会对患者施加沉重的负担。

公开(公告)号：US20080166771A1

公开(公告)日：2008-07-10

申请号：US11826116

申请日：2007-07-12

申请人： Sachiyo Tada ,  Tomokazu Yoshida ,  Keiichiro Shohmi ,  Masahiro Nishida ,  Kazuki Nakabayashi ,  Koichi Yamagata

发明人： Sachiyo Tada ,  Tomokazu Yoshida ,  Keiichiro Shohmi ,  Masahiro Nishida ,  Kazuki Nakabayashi ,  Koichi Yamagata

IPC分类号： C12P19/34 ,  C07H21/00 ,  C12N9/10

CPC分类号： C12Q1/6806 ,  C12Q1/6844 ,  C12Q2531/113 ,  C12Q2527/127 ,  C12Q2525/119

摘要： In detecting a nucleic acid by using the nucleic acid amplification method, it is intended to shorten the time required for the measurement so as to systematically and efficiently examine a gene, etc. To achieve this object, use is made of a method of directly amplifying a nucleic acid by treating a collected biological sample wherein a means of inhibiting the degradation activity of the nucleic acid is introduced during or after the step of homogenizing the biological sample and thus the nucleic acid is directly amplified without isolating/purifying the nucleic acid component from the biological sample. In this method, the means of inhibiting the degradation activity of the nucleic acid is introduced at an acidic pH value (more specifically, from pH 2.5 to pH 5) with the use of a salt interacting with a substance inhibiting the nucleic acid amplification reaction and/or the nucleic acid component so as to directly amplify the nucleic acid without isolating/purifying the nucleic acid component.

摘要翻译： 通过使用核酸扩增法检测核酸，旨在缩短测定所需的时间，从而系统地且有效地检查基因等。为了达到这个目的，使用直接扩增方法 通过处理收集的生物样品的核酸，其中在均化生物样品的步骤期间或之后引入抑制核酸降解活性的手段，因此核酸直接扩增而不从核酸组分分离/纯化 生物样品。 在该方法中，使用与抑制核酸扩增反应的物质相互作用的盐，在酸性pH值（更具体地，pH 2.5〜pH 5））下引入抑制核酸降解活性的方法，以及 /或核酸组分，以便直接扩增核酸而不分离/纯化核酸组分。

公开(公告)号：US20060228098A1

公开(公告)日：2006-10-12

申请号：US11400397

申请日：2006-04-06

申请人： Tomokazu Yoshida

发明人： Tomokazu Yoshida

IPC分类号： G03B13/00

CPC分类号： H04N5/23212 ,  G02B7/102 ,  G02B7/346 ,  G02B15/177 ,  H04N5/335

摘要： A focus detection apparatus includes an image sensor that applies photoelectric conversion to incident light and accumulates charge, a first output unit that outputs electric signals based on the charge accumulated in the image sensor through multiple channels, and a focus detection unit that detects a phase difference on the basis of the electric signals outputted from the plurality of channels.

公开(公告)号：US08265473B2

公开(公告)日：2012-09-11

申请号：US12940959

申请日：2010-11-05

申请人： Tomokazu Yoshida

发明人： Tomokazu Yoshida

IPC分类号： G03B3/00

CPC分类号： G03B7/095

摘要： A camera is provided which is adapted to receive a photographic lens having a diaphragm. The camera includes a detection unit configured to execute electric charge accumulation for detecting a defocus amount when the diaphragm is in a state having a first aperture value, and a controller configured to, when causing the diaphragm to perform an aperture reducing operation from the first aperture value to a second aperture value, cause the diaphragm to start the aperture reducing operation without waiting for completion of the electric charge accumulation for detecting the defocus amount by the detection unit.

摘要翻译： 提供了适于接收具有隔膜的摄影镜头的照相机。 摄像机包括：检测单元，被配置为执行电荷累积，用于当所述隔膜处于具有第一孔径值的状态时检测散焦量;以及控制器，被配置为当使所述隔膜从所述第一孔径执行孔径减小操作时 值到第二光圈值，使得光阑开始光圈减小操作，而不用等待完成由检测单元检测散焦量的电荷累积。

公开(公告)号：US20110050955A1

公开(公告)日：2011-03-03

申请号：US12870598

申请日：2010-08-27

申请人： Tomokazu Yoshida

发明人： Tomokazu Yoshida

IPC分类号： H04N5/76 ,  G02B7/04

CPC分类号： H04N5/23293 ,  H04N1/32128 ,  H04N5/232 ,  H04N5/23209 ,  H04N9/8233 ,  H04N21/4223 ,  H04N21/4334 ,  H04N21/435 ,  H04N21/4424 ,  H04N2101/00 ,  H04N2201/3204 ,  H04N2201/3277

摘要： An imaging apparatus according to the present invention can generate an image from an output of an image sensor to record the image as an image file, and record first information and second information onto the image file as additional information. Accordingly, the imaging apparatus can provide a user or a staff person of a repair center with useful information regarding a failure, repair, and replacement that conventionally could not be provided.

摘要翻译： 根据本发明的成像装置可以从图像传感器的输出生成图像以将图像记录为图像文件，并将第一信息和第二信息记录到图像文件上作为附加信息。 因此，成像装置可以向维修中心的用户或工作人员提供关于通常不能提供的故障，修理和替换的有用信息。

公开(公告)号：US20070264624A1

公开(公告)日：2007-11-15

申请号：US11747077

申请日：2007-05-10

申请人： Hideki Ishihara ,  Tomokazu Yoshida ,  Masatoshi Yamasaki ,  Sachiyo Tada

发明人： Hideki Ishihara ,  Tomokazu Yoshida ,  Masatoshi Yamasaki ,  Sachiyo Tada

IPC分类号： C12Q1/00

CPC分类号： C07K16/40 ,  C12N9/1205 ,  C12Q1/48 ,  C12Q1/485 ,  G01N33/57407 ,  G01N33/57484 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/9121

摘要： A method for determining the activity of a cell cycle regulatory factor comprising the steps of preparing a sample for measuring a cyclin-dependent kinase/cyclin complex from living cells; reacting adenosine 5′-O-(3-thiotriphosphate) (ATP-γ S) with a substrate for the cyclin-dependent kinase in presence of the sample in order to introduce a monothiophosphate group into a serine or threonine residue of the substrate; labeling the substrate by coupling a labeling fluorophore or a labeling enzyme with a sulfur atom of the introduced monothiophosphate group; measuring the amount of fluorescence from the labeling fluorophore labeling the substrate, or reacting the labeling enzyme labeling the substrate with a substance which generates an optically detectable product by reaction with the labeling enzyme and optically measuring the amount of the generated product; and calculating the activity of the cyclin-dependent kinase from the measured amount of fluorescence or the measured amount of the generated product with reference to a pre-produced reference curve.

摘要翻译： 一种确定细胞周期调控因子活性的方法，包括以下步骤：制备用于从活细胞测量细胞周期蛋白依赖性激酶/细胞周期蛋白复合物的样品; 在样品存在下使腺苷5'-O-（3-硫代三磷酸）（ATP-γS）与用于细胞周期蛋白依赖性激酶的底物反应，以将一硫代磷酸酯基团引入底物的丝氨酸或苏氨酸残基中; 通过将标记的荧光团或标记酶与引入的一硫代磷酸酯基团的硫原子偶联来标记底物; 测量来自标记荧光团的荧光标记基质的荧光量，或使标记基质的标记酶与通过与标记酶反应产生可光学检测的产物并光学测量产生的产物的量的物质反应; 并且根据测量的荧光量或参考预先产生的参考曲线测量产生的产物的量计算细胞周期蛋白依赖性激酶的活性。