公开(公告)号：US20190062825A1

公开(公告)日：2019-02-28

申请号：US15996084

申请日：2018-06-01

申请人： University of Washington ,  The UAB Research Foundation

发明人： Jens H. Gundlach ,  Michael Niederweis ,  Thomas Z. Butler ,  Mikhail Pavlenok ,  Mark A. Troll ,  Suja Sukumaran

IPC分类号： C12Q1/6869 ,  A61K39/04 ,  G01N33/487 ,  G01N27/447 ,  G01N33/68 ,  C12N13/00 ,  C07K14/35 ,  A61K39/00 ,  G01N24/00

摘要： Provided herein are Mycobacterium smegmatis porin nanopores, systems that comprise these nanopores, and methods of using and making these nanopores. Such nanopores may be wild-type MspA porins, mutant MspA porins, wild-type MspA paralog porins, wild-type MspA homolog porins, mutant MspA paralog porins, mutant MspA homolog porins, or single-chain Msp porins. Also provided are bacterial strains capable of inducible Msp porin expression.

公开(公告)号：US09624275B2

公开(公告)日：2017-04-18

申请号：US14318072

申请日：2014-06-27

申请人： University of Washington ,  The UAB Research Foundation

发明人： Jens H. Gundlach ,  Michael Niederweis ,  Thomas Z. Butler ,  Mikhail Pavlenok ,  Mark A. Troll ,  Suja Sukumaran

IPC分类号： A61K39/04 ,  A61K39/02 ,  C12Q1/70 ,  C07K14/35 ,  C12N13/00 ,  G01N33/68 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  A61K39/00 ,  A61K39/04 ,  C07K14/35 ,  C12N13/00 ,  G01N24/00 ,  G01N27/44704 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/6872 ,  C12Q2523/31 ,  C12Q2565/631

摘要： Provided herein are Mycobacterium smegmatis porin nanopores, systems that comprise these nanopores, and methods of using and making these nanopores. Such nanopores may be wild-type MspA porins, mutant MspA porins, wild-type MspA paralog porins, wild-type MspA homolog porins, mutant MspA paralog porins, mutant MspA homolog porins, or single-chain Msp porins. Also provided are bacterial strains capable of inducible Msp porin expression.

公开(公告)号：US09534024B2

公开(公告)日：2017-01-03

申请号：US14216349

申请日：2014-03-17

申请人： University of Washington ,  The UAB Research Foundation

发明人： Jens H. Gundlach ,  Michael Niederweis ,  Thomas Z. Butler ,  Mikhail Pavlenok ,  Mark A. Troll ,  Suja Sukumaran

IPC分类号： C07H21/02 ,  C07H21/04 ,  A61K39/04 ,  C07K14/35 ,  C12N13/00 ,  G01N33/68 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  A61K39/00 ,  A61K39/04 ,  C07K14/35 ,  C12N13/00 ,  G01N24/00 ,  G01N27/44704 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/6872 ,  C12Q2523/31 ,  C12Q2565/631

摘要： Provided herein are Mycobacterium smegmatis porin nanopores, systems that comprise these nanopores, and methods of using and making these nanopores. Such nanopores may be wild-type MspA porins, mutant MspA porins, wild-type MspA paralog porins, wild-type MspA homolog porins, mutant MspA paralog porins, mutant MspA homolog porins, or single-chain Msp porins. Also provided are bacterial strains capable of inducible Msp porin expression.

摘要翻译： 本文提供了耻垢分枝杆菌孔蛋白纳米孔，包含这些纳米孔的体系，以及使用和制备这些纳米孔的方法。 这样的纳米孔可以是野生型MspA孔蛋白，突变MspA孔蛋白，野生型MspA旁系同源孔蛋白，野生型MspA同源孔蛋白，突变MspA旁系同源孔蛋白，突变MspA同源孔蛋白或单链Msp孔蛋白。 还提供能够诱导Msp孔蛋白表达的细菌菌株。

公开(公告)号：US20140256913A1

公开(公告)日：2014-09-11

申请号：US14216349

申请日：2014-03-17

申请人： The UAB Research Foundation ,  University of Washington

发明人： Jens H. Gundlach ,  Michael Niederweis ,  Thomas Z. Butler ,  Mikhail Pavlenok ,  Mark A. Troll ,  Suja Sukumaran

IPC分类号： C07K14/35

CPC分类号： C12Q1/6869 ,  A61K39/00 ,  A61K39/04 ,  C07K14/35 ,  C12N13/00 ,  G01N24/00 ,  G01N27/44704 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/6872 ,  C12Q2523/31 ,  C12Q2565/631

摘要： Provided herein are Mycobacterium smegmatis porin nanopores, systems that comprise these nanopores, and methods of using and making these nanopores. Such nanopores may be wild-type MspA porins, mutant MspA porins, wild-type MspA paralog porins, wild-type MspA homolog porins, mutant MspA paralog porins, mutant MspA homolog porins, or single-chain Msp porins. Also provided are bacterial strains capable of inducible Msp porin expression.

公开(公告)号：US20240360502A1

公开(公告)日：2024-10-31

申请号：US18769519

申请日：2024-07-11

申请人： University of Washington through its Center for Commercialization ,  Illumina, Inc.

发明人： Jens H. Gundlach ,  Andrew Laszlo ,  Ian Derrington ,  Jeffrey G. Mandell

IPC分类号： C12Q1/6869 ,  B01D57/02 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  B01D57/02 ,  G01N33/48721

摘要： The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.

公开(公告)号：US10858700B2

公开(公告)日：2020-12-08

申请号：US14419217

申请日：2013-08-02

申请人： University of Washington through its Center for Commercialization

发明人： Jens H. Gundlach ,  Andrew Laszlo

IPC分类号： C12Q1/68 ,  C12Q1/6869

摘要： The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.

公开(公告)号：US10017814B2

公开(公告)日：2018-07-10

申请号：US14915611

申请日：2014-09-02

申请人： University of Washington through its Center for Commercialization ,  Illumina, Inc.

发明人： Jens H. Gundlach ,  Andrew Laszlo ,  Ian Derrington ,  Jeffrey G. Mandell

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  B01D57/02 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  B01D57/02 ,  G01N33/48721 ,  C12Q2521/101 ,  C12Q2523/303 ,  C12Q2525/107 ,  C12Q2525/113 ,  C12Q2525/119 ,  C12Q2565/631 ,  C12Q2523/125

摘要： The present disclosure provides method and systems for improving nanopore-based analysis of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.

公开(公告)号：US20170227494A1

公开(公告)日：2017-08-10

申请号：US15432897

申请日：2017-02-14

申请人： University of Washington

发明人： Jens H. Gundlach ,  Ian M. Derrington ,  Marcus D. Collins

IPC分类号： G01N27/447 ,  C12Q1/68

CPC分类号： G01N27/44791 ,  B01D69/06 ,  B01D2325/02 ,  B01D2325/24 ,  B82Y5/00 ,  B82Y15/00 ,  C07K14/35 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/44704 ,  G01N27/44726 ,  G01N27/44747 ,  G01N27/44786 ,  G01N33/48721 ,  Y10S977/712 ,  Y10S977/714 ,  Y10S977/781 ,  Y10S977/924

摘要： Provided herein are methods and systems pertaining to sequencing units of analytes using nanopores. In general, arresting constructs are used to modify an analyte such that the modified analyte pauses in the opening of a nanopore. During such a pause, an ion current level is obtained that corresponds to a unit of the analyte. After altering the modified analyte such that the modified analyte advances through the opening, another arresting construct again pauses the analyte, allowing for a second ion current level to be obtained that represents a second unit of the analyte. This process may be repeated until each unit of the analyte is sequenced. Systems for performing such methods are also disclosed.

公开(公告)号：US20240085372A1

公开(公告)日：2024-03-14

申请号：US18475534

申请日：2023-09-27

申请人： University of Washington

发明人： Jens H. Gundlach ,  Ian Michael Derrington ,  Andrew Laszlo ,  Jonathan Craig ,  Henry Brinkerhoff

IPC分类号： G01N27/447 ,  G01N33/487 ,  G01N33/68

CPC分类号： G01N27/44743 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/6818

摘要： Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

公开(公告)号：US11821033B2

公开(公告)日：2023-11-21

申请号：US17111167

申请日：2020-12-03

申请人： University of Washington through its Center for Commercialization

发明人： Jens H. Gundlach ,  Andrew Laszlo

IPC分类号： C12Q1/6869

CPC分类号： C12Q1/6869 ,  C12Q1/6869 ,  C12Q2523/31 ,  C12Q2565/631

摘要： The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.