公开(公告)号：US20120325680A1

公开(公告)日：2012-12-27

申请号：US13584695

申请日：2012-08-13

申请人： Wataru Kubo ,  Tsuyoshi Nomoto

发明人： Wataru Kubo ,  Tsuyoshi Nomoto

IPC分类号： G01N27/26

CPC分类号： C12Q1/008 ,  C12Q1/26 ,  G01N33/573 ,  G01N33/6893 ,  G01N2800/30

摘要： The invention is to provide an information acquisition apparatus for acquiring information relating to at least one of an oxidized form concentration, a reduced form concentration and a ratio of the concentrations of thioredoxin, useful for judging a stress level, and a stress level information acquisition apparatus and a stress level judging method utilizing the same. A reaction by an enzyme or the like catalyzing an redox reaction of thioredoxins is used to measure at least one of an oxidized form concentration, a reduced form concentration and a concentration ratio of thioredoxins, and data of such measurement is used for judging the stress level of a subject person.

摘要翻译： 本发明提供一种信息获取装置，用于获取关于用于判断应力水平的硫氧还蛋白浓度的氧化形式浓度，浓缩形式浓度和浓度之比以及应力水平信息获取装置中的至少一种的信息 以及利用该判定方法的应力水平判断方法。 催化硫氧还蛋白的氧化还原反应的酶等的反应用于测定氧化形式浓度，还原形式浓度和硫氧还蛋白浓度比中的至少一种，并且使用这种测量的数据来判断应力水平 的主题人。

公开(公告)号：US20080102323A1

公开(公告)日：2008-05-01

申请号：US11837074

申请日：2007-08-10

申请人： Wataru Kubo ,  Tsuyoshi Nomoto

发明人： Wataru Kubo ,  Tsuyoshi Nomoto

IPC分类号： C12M1/40 ,  H01M8/02 ,  C12P21/04 ,  H01M4/00

CPC分类号： C12Q1/001 ,  H01M4/90 ,  H01M8/16 ,  Y02E60/527 ,  Y02P70/56

摘要： An amino acid capable of coordinately binding to a metallic complex is introduced to an active site within the enzyme or another controlled position near the active site. Therefore, when a mediator is introduced into the enzyme, a mediator-introduced position is controlled to be held at or near the active site.

摘要翻译： 能够与金属配合物配位结合的氨基酸被引入活性位点附近的酶或其他控制位置的活性位点。 因此，当将介体引入酶时，介导介质位置被控制为保持在活性位点处或附近。

公开(公告)号：US08329011B2

公开(公告)日：2012-12-11

申请号：US12297173

申请日：2007-06-18

申请人： Tsuyoshi Nomoto ,  Wataru Kubo

发明人： Tsuyoshi Nomoto ,  Wataru Kubo

IPC分类号： C12N11/00 ,  C12N11/06

CPC分类号： G01N27/447 ,  C12Q1/6869 ,  C12Q2565/607

摘要： The present invention is to reduce a false signal in an apparatus for electrochemically decoding a base sequence of DNA, which false signal is caused by a phenomenon that even unreacted nucleotide 5′-triphosphate derivatives remaining in the solution are electrochemically converted on an electrode. The present invention provides a polymerase-immobilized electrode that comprises an electroconductive substrate and a polymerase unit immobilized on the surface of the electroconductive substrate, wherein the polymerase unit comprises a polymerase part, an anchor part and an electroconductive part, which are linked in an order of the polymerase part, the anchor part and the electroconductive part, or in an order of the polymerase part, the electroconductive part and the anchor part; and the polymerase unit is immobilized on the electroconductive substrate by the anchor part; wherein a free end of the electroconductive part, not immobilized on the electroconductive substrate, is located in the vicinity of an active site of the polymerase part.

摘要翻译： 本发明是为了减少用于电化学解码DNA碱基序列的装置中的假信号，该伪信号是由残留在溶液中的未反应的核苷酸5'-三磷酸衍生物在电极上进行电化学转化的现象引起的。 本发明提供一种聚合酶固定化电极，其包含导电性基质和固定在导电性基质表面的聚合酶单元，其中聚合酶单元包含聚合酶部分，锚定部分和导电部分，其按顺序连接 聚合酶部分，锚定部分和导电部分，或按照聚合酶部分，导电部分和锚固部分的顺序; 并且聚合酶单元通过锚定部分固定在导电基底上; 其中未固定在导电性基材上的导电性部分的自由端位于聚合酶部分的活性部位附近。

公开(公告)号：US07816025B2

公开(公告)日：2010-10-19

申请号：US11837074

申请日：2007-08-10

申请人： Wataru Kubo ,  Tsuyoshi Nomoto

发明人： Wataru Kubo ,  Tsuyoshi Nomoto

IPC分类号： H01M4/00 ,  C12M1/40

CPC分类号： C12Q1/001 ,  H01M4/90 ,  H01M8/16 ,  Y02E60/527 ,  Y02P70/56

摘要： An amino acid capable of coordinately binding to a metallic complex is introduced to an active site within the enzyme or another controlled position near the active site. Therefore, when a mediator is introduced into the enzyme, a mediator-introduced position is controlled to be held at or near the active site.

摘要翻译： 能够与金属配合物配位结合的氨基酸被引入活性位点附近的酶或其他控制位置的活性位点。 因此，当将介体引入酶时，介导介质位置被控制为保持在活性位点处或附近。

公开(公告)号：US20090134042A1

公开(公告)日：2009-05-28

申请号：US12297173

申请日：2007-06-18

申请人： Tsuyoshi Nomoto ,  Wataru Kubo

发明人： Tsuyoshi Nomoto ,  Wataru Kubo

IPC分类号： G01N27/327 ,  G01N27/26

CPC分类号： G01N27/447 ,  C12Q1/6869 ,  C12Q2565/607

摘要： The present invention is to reduce a false signal in an apparatus for electrochemically decoding a base sequence of DNA, which false signal is caused by a phenomenon that even unreacted nucleotide 5′-triphosphate derivatives remaining in the solution are electrochemically converted on an electrode. The present invention provides a polymerase-immobilized electrode that comprises an electroconductive substrate and a polymerase unit immobilized on the surface of the electroconductive substrate, wherein the polymerase unit comprises a polymerase part, an anchor part and an electroconductive part, which are linked in an order of the polymerase part, the anchor part and the electroconductive part, or in an order of the polymerase part, the electroconductive part and the anchor part; and the polymerase unit is immobilized on the electroconductive substrate by the anchor part; wherein a free end of the electroconductive part, not immobilized on the electroconductive substrate, is located in the vicinity of an active site of the polymerase part.

摘要翻译： 本发明是为了减少用于电化学解码DNA碱基序列的装置中的假信号，该伪信号是由残留在溶液中的未反应的核苷酸5'-三磷酸衍生物在电极上进行电化学转化的现象引起的。 本发明提供一种聚合酶固定化电极，其包含导电性基质和固定在导电性基质表面的聚合酶单元，其中聚合酶单元包含聚合酶部分，锚定部分和导电部分，其按顺序连接 聚合酶部分，锚定部分和导电部分，或按照聚合酶部分，导电部分和锚固部分的顺序; 并且聚合酶单元通过锚定部分固定在导电基底上; 其中未固定在导电性基材上的导电性部分的自由端位于聚合酶部分的活性部位附近。

公开(公告)号：US20080138663A1

公开(公告)日：2008-06-12

申请号：US11947271

申请日：2007-11-29

申请人： Wataru Kubo ,  Tsuyoshi Nomoto

发明人： Wataru Kubo ,  Tsuyoshi Nomoto

IPC分类号： H01M8/16 ,  C25B11/06

CPC分类号： H01M8/16 ,  C12N11/16 ,  C12N13/00 ,  C12Q1/001 ,  C12Q1/02 ,  Y02E60/527

摘要： The present invention is directed to overcoming a problem of rate-limiting diffusion of a substrate toward an enzyme, which is a constituent of a microorganism, while ensuring proliferation of the microorganism, in a microbial electrode using a microorganism as an electrode catalyst. A microbial electrode is prepared using a microorganism expressing an enzyme on the surface layer of the cell membrane or cell wall thereof.

摘要翻译： 本发明旨在克服在使用微生物作为电极催化剂的微生物电极的同时确保微生物的增殖的基板向作为微生物的成分的酶的扩散速率的限制的问题。 使用表达酶的微生物在细胞膜或其细胞壁的表面层上制备微生物电极。

公开(公告)号：US20130051534A1

公开(公告)日：2013-02-28

申请号：US13591616

申请日：2012-08-22

申请人： Kohei Okamoto ,  Wataru Kubo ,  Hirokatsu Miyata

发明人： Kohei Okamoto ,  Wataru Kubo ,  Hirokatsu Miyata

IPC分类号： G21K1/00

CPC分类号： G21K1/062 ,  B82Y10/00

摘要： An X-ray waveguide for propagation of an X-ray therethrough includes a core and a cladding. The core has a periodic structure in which plural substances having different refractive-index real parts are periodically arrayed in a direction perpendicular to an X-ray guiding direction. Given that a maximum length of the core in the X-ray guiding direction is l, a maximum thickness of the core is t, and a Bragg angle of the periodic structure for the X-ray is θB(°), at least one end surface of a core region in the X-ray guiding direction is inclined at an inclination angle φ(°), which satisfies tan−1(t/l)

摘要翻译： 用于传播X射线的X射线波导包括芯和包层。 芯具有周期性结构，其中具有不同折射率实部的多个物质在与X射线引导方向垂直的方向上周期性排列。 假设在X射线引导方向上的芯的最大长度为l，则芯的最大厚度为t，并且X射线的周期性结构的布拉格角为至少; B（°）至少 在X射线引导方向上的芯区域的一个端面以倾斜角度（°）倾斜，其满足tan-1（t / l）<＆phgr; <90°-θ; B，相对于 在包含平行于X射线引导方向的方向和垂直于芯和包层之间的界面的方向的平面中的芯和包层之间的界面。

公开(公告)号：US20120328082A1

公开(公告)日：2012-12-27

申请号：US13582578

申请日：2010-06-01

申请人： Wataru Kubo ,  Kohei Okamoto ,  Atsushi Komoto ,  Hirokatsu Miyata

发明人： Wataru Kubo ,  Kohei Okamoto ,  Atsushi Komoto ,  Hirokatsu Miyata

IPC分类号： G21K1/06 ,  B05D5/00

CPC分类号： G21K1/062 ,  B05D5/00 ,  G21K1/06 ,  G21K2201/061 ,  H01J2235/168

摘要： Provided is an X-ray mirror, a method of producing the X-rat mirror, and an X-ray apparatus. The X-ray mirror comprises: a substrate; and an X-ray reflecting structure formed of multiple regions present on the substrate, in which the X-ray reflecting structure comprises a mesostructured film that has the multiple regions having different structural periods in a normal direction of the substrate. Thus, there can be reduced the absorption loss of an X-ray of the mirror that reflects X-rays having different energies.

摘要翻译： 本发明提供一种X射线镜，X射线镜的制造方法以及X射线装置。 X射线镜包括：基片; 以及由存在于基板上的多个区域形成的X射线反射结构，其中，X射线反射结构包括具有在基板的法线方向上具有不同结构周期的多个区域的介孔结构膜。 因此，可以减少反射具有不同能量的X射线的反射镜的X射线的吸收损失。

公开(公告)号：US20120269327A1

公开(公告)日：2012-10-25

申请号：US13443054

申请日：2012-04-10

申请人： Kohei Okamoto ,  Atsushi Komoto ,  Hirokatsu Miyata ,  Wataru Kubo ,  Takashi Noma

发明人： Kohei Okamoto ,  Atsushi Komoto ,  Hirokatsu Miyata ,  Wataru Kubo ,  Takashi Noma

IPC分类号： G21K1/00

CPC分类号： G21K1/067 ,  G21K1/062

摘要： An X-ray waveguide includes a cladding and a core. The core has a periodic structure formed in at least one period direction. The periodic structure includes periodically arranged members made of material having different refractive index real parts. The core is surrounded by the cladding in the plane perpendicular to a wave-guiding direction. The Bragg angle obtained from the periodicity of the periodic structure is smaller than the total reflection critical angle at which X-rays are incident on the interface between the cladding and the core. The at least one period direction is the direction of at least one fundamental vector expressing the periodicity of the periodic structure in a plane of the core perpendicular to the wave-guiding direction.

摘要翻译： X射线波导包括包层和芯。 芯具有在至少一个周期方向上形成的周期性结构。 周期性结构包括由具有不同折射率实部的材料制成的周期性布置的构件。 芯在垂直于波导方向的平面中被包层包围。 从周期结构的周期性获得的布拉格角小于X射线入射在包层和芯之间的界面上的全反射临界角。 至少一个周期方向是表示在垂直于波导方向的芯的平面中表示周期性结构的周期性的至少一个基本矢量的方向。

公开(公告)号：US20110129065A1

公开(公告)日：2011-06-02

申请号：US12916025

申请日：2010-10-29

申请人： Atsushi Komoto ,  Hirokatsu Miyata ,  Wataru Kubo

发明人： Atsushi Komoto ,  Hirokatsu Miyata ,  Wataru Kubo

IPC分类号： G21K1/06 ,  B05D5/06

CPC分类号： G21K1/06

摘要： An X-ray monochromator including: a substrate having a concave surface; and an inorganic oxide film formed on the concave surface and having a plurality of pores, in which the plurality of pores of the inorganic oxide film being laid periodically in a stacked manner in the normal directions of the concave surface, and in which the plurality of pores being cylindrical is provided. The X-ray monochromator shows an excellent X-ray spectroscopic performance.

摘要翻译： 一种X射线单色仪，包括：具有凹面的基板; 以及形成在所述凹面上并具有多个孔的无机氧化物膜，其中，所述无机氧化物膜的多个孔沿着所述凹面的法线方向以层叠的方式周期性地放置，并且其中， 提供了圆柱形的孔。 X射线单色仪显示出优异的X射线光谱性能。