公开(公告)号：US20250114786A1

公开(公告)日：2025-04-10

申请号：US18985183

申请日：2024-12-18

Applicant: Waters Technologies Corporation

Inventor： Mathew H. DeLano ,  Matthew Lauber ,  Andrew Wyatt Schmudlach ,  Daniel P. Walsh ,  Yeliz Tunc Sarisozen ,  Jonathan Belanger ,  Darryl W. Brousmiche ,  Andrew Bates-Harrison ,  Nicole L. Lawrence

IPC: B01L3/00 ,  C09D143/04 ,  C23C16/56 ,  G01N30/60

Abstract: The present disclosure is directed to a coating process for chromatographic surfaces. Embodiments of the present disclosure feature a two-step, vapor-liquid phase organosilane deposition method for creating a hydrophilic, non-ionic surface in a chromatographic system.

公开(公告)号：US12201975B2

公开(公告)日：2025-01-21

申请号：US17502483

申请日：2021-10-15

Applicant: Waters Technologies Corporation

Inventor： Mathew H. DeLano ,  Matthew Lauber ,  Andrew Wyatt Schmudlach ,  Daniel P. Walsh ,  Yeliz Tunc Sarisozen ,  Jonathan Belanger ,  Darryl W. Brousmiche ,  Andrew Bates-Harrison ,  Nicole L. Lawrence

IPC: G01N30/56 ,  B01L3/00 ,  C09D143/04 ,  C23C16/56 ,  G01N30/60

Abstract: The present disclosure is directed to a coating process for chromatographic surfaces. Embodiments of the present disclosure feature a two-step, vapor-liquid phase organosilane deposition method for creating a hydrophilic, non-ionic surface in a chromatographic system.

公开(公告)号：US20240367072A1

公开(公告)日：2024-11-07

申请号：US18654384

申请日：2024-05-03

Applicant: Waters Technologies Corporation

Inventor： Kevin Wyndham ,  Beatrice Muriithi ,  Oksana Tchoul ,  Yeliz Tunc Sarisozen ,  Mingcheng Xu ,  Matthew Lauber

IPC: B01D15/38 ,  B01J20/26 ,  B01J20/28

Abstract: The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 micrometers and being functionalized with an immunoglobulin-binding protein. The functionalized particles of the present disclosure can be used to purify a range of immunoglobulins with affinity to the immunoglobulin-binding protein.

公开(公告)号：US20240280450A1

公开(公告)日：2024-08-22

申请号：US18643216

申请日：2024-04-23

Applicant: Waters Technologies Corporation

Inventor： Xiaoxiao Liu ,  Matthew Lauber ,  Michael Donegan

IPC: G01N1/40 ,  B01D15/42 ,  B01J20/28 ,  B01J20/281 ,  B01J20/34 ,  G01N30/00 ,  G01N30/06 ,  G01N30/16

CPC classification number: G01N1/405 ,  B01D15/426 ,  B01J20/28052 ,  B01J20/281 ,  B01J20/3475 ,  G01N30/06 ,  G01N30/16 ,  B01J2220/54 ,  G01N2030/009 ,  G01N2030/067

Abstract: The present technology relates to a method for improved recovery of phosphorylated compounds (e.g., phosphorylated glycans). In particular, the present technology utilizes certain chelator additives in a solution to wash and elute the phosphorylated compounds from a solid phase extraction cartridge.

公开(公告)号：US20240240170A1

公开(公告)日：2024-07-18

申请号：US18414679

申请日：2024-01-17

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Matthew Lauber ,  Scott Mccall ,  Pamela Iraneta ,  Kerri Smith

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/101 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/315

Abstract: The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.

公开(公告)号：US20230258649A1

公开(公告)日：2023-08-17

申请号：US18167152

申请日：2023-02-10

Applicant: Waters Technologies Corporation

Inventor： Matthew Lauber ,  Wenjing Li

IPC: G01N33/68

CPC classification number: G01N33/6821 ,  G01N2333/976

Abstract: The present invention relates to a method for analyzing a sample, the method comprising: (a) incubating a sample comprising an analyte with at least one enzyme to produce a digestion mixture comprising fragments of the analyte; (b) loading the digestion mixture onto a reversed-phase chromatography column; and (c) performing reversed-phase chromatography on the digestion mixture, wherein the at least one enzyme is hydrophobically modified to increase a retention time of the at least one enzyme such that the at least one enzyme elutes from the reversed-phase chromatography column later than the fragments of the analyte.

公开(公告)号：US11448652B2

公开(公告)日：2022-09-20

申请号：US16562173

申请日：2019-09-05

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu ,  Matthew Lauber

IPC: G01N33/00 ,  G01N33/58 ,  C07D401/12 ,  C07D207/46 ,  C07C261/00 ,  G01N33/533

Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. In particular, compounds for use as a reagent for rapid fluorescence tagging of biomolecules and enhanced MS signaling are provided. The compounds may have optical centers and therefore may occur in different enantiomeric and diastereomeric configurations. These MS active, fluorescent compounds may have three functional components: (a) a tertiary amino group or other MS active atom; (b) a highly fluorescent moiety, and (c) a reactive group that rapidly reacts with amines. The reactive group provides rapid tagging of desired bio-molecules. The fluorescent moiety provides the fluorescent signal. The tertiary amino group provides the MS signal.

公开(公告)号：US20210171932A1

公开(公告)日：2021-06-10

申请号：US17112502

申请日：2020-12-04

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Matthew Lauber ,  Scott Mccall ,  Pamela Iraneta ,  Kerri Smith

IPC: C12N15/10 ,  C12N15/113

Abstract: The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.

公开(公告)号：US20140350263A1

公开(公告)日：2014-11-27

申请号：US14458760

申请日：2014-08-13

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu ,  Matthew Lauber

IPC: G01N33/58 ,  C07D207/46 ,  C07C261/00 ,  C07D401/12

CPC classification number: G01N33/582 ,  C07C261/00 ,  C07D207/46 ,  C07D401/12 ,  G01N33/533 ,  G01N2400/00 ,  G01N2400/12 ,  G01N2458/15 ,  G01N2458/20

Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described.

Abstract translation: 教导并描述了包含具有用于与标记生物分子如N-聚糖有用的胺反应的活性官能团的活性荧光分子的试剂和其用途。