公开(公告)号：US20180088094A1

公开(公告)日：2018-03-29

申请号：US15716659

申请日：2017-09-27

Applicant: Waters Technologies Corporation

Inventor： Ying-Qing Yu ,  Henry Y. Shion ,  Scott J. Berger ,  Jing Fang ,  Liuxi Chen ,  Min Du

IPC: G01N30/72 ,  H01J49/00

CPC classification number: G01N30/72 ,  G01N33/6848 ,  G01N2030/062 ,  G01N2030/8813 ,  H01J49/009

Abstract: The present disclosure relates generally to a method of multiple attribute monitoring for biological and other complex compounds using a chromatography-optical detector-mass spectrometry method. The mass spectrometry method can use a high resolution mass spectrometer. The methodology utilizes similar analytical techniques and instruments for both the characterization and the monitoring of biological and other complex compounds.

公开(公告)号：US11448652B2

公开(公告)日：2022-09-20

申请号：US16562173

申请日：2019-09-05

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu ,  Matthew Lauber

IPC: G01N33/00 ,  G01N33/58 ,  C07D401/12 ,  C07D207/46 ,  C07C261/00 ,  G01N33/533

Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. In particular, compounds for use as a reagent for rapid fluorescence tagging of biomolecules and enhanced MS signaling are provided. The compounds may have optical centers and therefore may occur in different enantiomeric and diastereomeric configurations. These MS active, fluorescent compounds may have three functional components: (a) a tertiary amino group or other MS active atom; (b) a highly fluorescent moiety, and (c) a reactive group that rapidly reacts with amines. The reactive group provides rapid tagging of desired bio-molecules. The fluorescent moiety provides the fluorescent signal. The tertiary amino group provides the MS signal.

公开(公告)号：US20140350263A1

公开(公告)日：2014-11-27

申请号：US14458760

申请日：2014-08-13

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu ,  Matthew Lauber

IPC: G01N33/58 ,  C07D207/46 ,  C07C261/00 ,  C07D401/12

CPC classification number: G01N33/582 ,  C07C261/00 ,  C07D207/46 ,  C07D401/12 ,  G01N33/533 ,  G01N2400/00 ,  G01N2400/12 ,  G01N2458/15 ,  G01N2458/20

Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described.

Abstract translation: 教导并描述了包含具有用于与标记生物分子如N-聚糖有用的胺反应的活性官能团的活性荧光分子的试剂和其用途。

公开(公告)号：US20140179011A1

公开(公告)日：2014-06-26

申请号：US14193418

申请日：2014-02-28

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu

IPC: G01N33/58

CPC classification number: G01N33/58 ,  C07D207/46 ,  C09B19/00 ,  C09B57/02 ,  C09B57/08 ,  G01N33/582 ,  G01N2400/00 ,  G01N2400/12 ,  G01N2458/15 ,  G01N2458/20 ,  Y10T436/143333

Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described.

公开(公告)号：US20200174008A1

公开(公告)日：2020-06-04

申请号：US16562173

申请日：2019-09-05

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu ,  Matthew Lauber

IPC: G01N33/58 ,  C07D401/12 ,  C07D207/46 ,  C07C261/00 ,  G01N33/533

Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described.

公开(公告)号：US10436790B2

公开(公告)日：2019-10-08

申请号：US14458760

申请日：2014-08-13

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu ,  Matthew Lauber

IPC: G01N33/00 ,  G01N33/58 ,  C07D401/12 ,  C07D207/46 ,  C07C261/00 ,  G01N33/533

Abstract: Mass spectrometry (MS) active, fluorescent rapid tagging reagent is provided having three substituent groups: (a) a tertiary amino group or other MS active atom; (b) a highly fluorescent moiety; and (c) a reactive group that can react with an amine. The reactive group provides rapid tagging of desired bio-molecules. The fluorescent moiety provides the fluorescent signal. The tertiary amino group provides the MS signal.

公开(公告)号：US10416166B2

公开(公告)日：2019-09-17

申请号：US14342131

申请日：2012-09-28

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu

IPC: G01N33/00 ,  G01N33/58 ,  C07D207/46 ,  C09B19/00 ,  C09B57/02 ,  C09B57/08

Abstract: Reagents comprising MS active, fluorescent molecules with an activate functionality for reaction with amines useful in rapid tagging of biomolecules such as N-glycans, proteins, peptides, and amino acids, and uses thereof are taught and described. These MS active, fluorescent molecules may have three functional components such as a tertiary amino group or other MS active atom, a highly fluorescent moiety, and a functional group that rapidly reacts with amines.

公开(公告)号：US09772333B2

公开(公告)日：2017-09-26

申请号：US14193418

申请日：2014-02-28

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu

IPC: G01N33/00 ,  G01N33/58 ,  C07D207/46

CPC classification number: G01N33/58 ,  C07D207/46 ,  C09B19/00 ,  C09B57/02 ,  C09B57/08 ,  G01N33/582 ,  G01N2400/00 ,  G01N2400/12 ,  G01N2458/15 ,  G01N2458/20 ,  Y10T436/143333

Abstract: Mass spectrometry (MS) active, fluorescent rapid tagging reagent is provided having three substituent groups: (a) a tertiary amino group or other MS active atom; (b) a highly fluorescent moiety; and (c) a reactive group that can react with an amine. The reactive group provides rapid tagging of desired bio-molecules. The fluorescent moiety provides the fluorescent signal. The tertiary amino group provides the MS signal.

公开(公告)号：US09115178B2

公开(公告)日：2015-08-25

申请号：US14034754

申请日：2013-09-24

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Ying-Qing Yu ,  Jennifer Fournier ,  John E. O'Gara

IPC: C07K1/36 ,  G01N30/34 ,  G01N30/60 ,  C12Q1/37 ,  G01N1/40 ,  G01N35/10

CPC classification number: C07K1/36 ,  C12Q1/37 ,  G01N1/405 ,  G01N1/4055 ,  G01N30/34 ,  G01N30/6069 ,  G01N35/1097

Abstract: Embodiments of the present invention are directed to articles of manufacture, devices, methods and apparatus for performing liquid chromatography featuring a chromatographic sorbent having one or more pentafluorophenyl groups, wherein said one or more pentafluorophenyl groups are a bonded phase on a sorbent selected from the group comprising silica, organic polymers or hybrid organic silane material and said pentafluorophenyl groups are in a mono-, bi-, and tridentate forms.

Abstract translation: 本发明的实施方案涉及用于进行液相色谱的制品，装置，方法和装置，其特征在于具有一个或多个五氟苯基的色谱吸附剂，其中所述一个或多个五氟苯基是选自组中的吸附剂上的键合相 包括二氧化硅，有机聚合物或杂化有机硅烷材料，所述五氟苯基是单 - ，二 - 和三齿形式。

公开(公告)号：US20140242709A1

公开(公告)日：2014-08-28

申请号：US14342131

申请日：2012-09-28

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu

IPC: G01N33/58

CPC classification number: G01N33/58 ,  C07D207/46 ,  C09B19/00 ,  C09B57/02 ,  C09B57/08 ,  G01N33/582 ,  G01N2400/00 ,  G01N2400/12 ,  G01N2458/15 ,  G01N2458/20 ,  Y10T436/143333

Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described.