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公开(公告)号：US08865459B2
公开(公告)日：2014-10-21
申请号：US12173472
申请日：2008-07-15
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
摘要： The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.
摘要翻译： 本发明涉及用于通过荧光测定分析样品中的核酸的核酸分析装置，其中通过光照射进行局部表面等离子体激光，并且其中用于核酸分析的核酸探针或核酸合酶 在样品中放置在产生表面等离子体的区域中。 本发明允许有效地产生表面等离子体激元的荧光增强效果，并且还能够在发挥荧光增强效果的区域中固定DNA探针或核酸合酶，从而可以进行 基本伸长反应的测量，而不用荧光分子去除未反应的底物。
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公开(公告)号：US20110081655A1
公开(公告)日：2011-04-07
申请号：US12997469
申请日：2009-05-13
CPC分类号： G01N21/648
摘要： An object of the present invention relates to distinguishing, from a fluorophore of an unreacted substrate, a single fluorophore attached to a nucleotide that is incorporated into a probe by a nucleic acid synthesis. The present invention relates to a nucleic acid analyzing device that analyzes a nucleic acid in sample by fluorescence, wherein a localized surface plasmon is generated by illumination, and a probe for analyzing the nucleic acid in the sample is on the site where the surface plasmon is generated. According to the present invention, since it is possible to efficiently produce fluorescence intensifying effects due to the surface plasmon and to immobilize the probe to a region within the reach of the fluorescence intensifying effects, it becomes possible to measure a nucleic acid synthesis without removing unreacted nucleotide to which fluorophores are attached.
摘要翻译： 本发明的目的在于从未反应的底物的荧光团区分与通过核酸合成并入探针中的核苷酸连接的单个荧光团。 本发明涉及通过荧光分析样品中的核酸的核酸分析装置，其中通过照射产生局部表面等离子体激元，并且用于分析样品中的核酸的探针位于表面等离子体为 生成。 根据本发明，由于可以有效地产生由于表面等离子体激元引起的荧光增强作用并且将探针固定在荧光增强作用范围内的区域，因此可以测量核酸合成而不去除未反应的 附着有荧光团的核苷酸。
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公开(公告)号：US20060134667A1
公开(公告)日：2006-06-22
申请号：US11274314
申请日：2005-11-16
CPC分类号： C12Q1/6806 , C12Q1/6827 , C12Q1/6834 , C12Q1/6886 , C12Q2600/158 , C12Q2525/143 , C12Q2521/119 , C12Q2525/149
摘要： The present invention relates to a method for detecting fusion gene transcripts resulting from chromosomal translocation. Specifically, the method of the present invention comprises allowing at least two or more probes, each of which contains a partial base sequence of exons which sandwich the breakpoint of a fusion gene or complementary base sequence thereof, and each of which immobilized on a support, to hybridize with a sample containing a nucleic acid derived from a fusion gene, thereby allowing the detection of two or more fusion genes at a time.
摘要翻译： 本发明涉及一种检测由染色体易位引起的融合基因转录物的方法。 具体地说，本发明的方法包括允许至少两个或更多个探针，每个探针含有夹心融合基因或其互补碱基序列的断点的外显子的部分碱基序列，并且每个探针固定在载体上， 与含有衍生自融合基因的核酸的样品杂交，从而一次检测两个或更多个融合基因。
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公开(公告)号：US08865403B2
公开(公告)日：2014-10-21
申请号：US12997469
申请日：2009-05-13
CPC分类号： G01N21/648
摘要： An object of the present invention relates to distinguishing, from a fluorophore of an unreacted substrate, a single fluorophore attached to a nucleotide that is incorporated into a probe by a nucleic acid synthesis. The present invention relates to a nucleic acid analyzing device that analyzes a nucleic acid in sample by fluorescence, wherein a localized surface plasmon is generated by illumination, and a probe for analyzing the nucleic acid in the sample is on the site where the surface plasmon is generated. According to the present invention, since it is possible to efficiently produce fluorescence intensifying effects due to the surface plasmon and to immobilize the probe to a region within the reach of the fluorescence intensifying effects, it becomes possible to measure a nucleic acid synthesis without removing unreacted nucleotide to which fluorophores are attached.
摘要翻译： 本发明的目的在于从未反应的底物的荧光团区分与通过核酸合成并入探针中的核苷酸连接的单个荧光团。 本发明涉及通过荧光分析样品中的核酸的核酸分析装置，其中通过照射产生局部表面等离子体激元，并且用于分析样品中的核酸的探针位于表面等离子体为 生成。 根据本发明，由于可以有效地产生由于表面等离子体激元引起的荧光增强作用并且将探针固定在荧光增强作用范围内的区域，因此可以测量核酸合成而不去除未反应的 附着有荧光团的核苷酸。
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15.发明申请APPARATUS AND METHOD FOR DETECTING TARGET SUBSTANCE, OR DEVICE USED FOR THESE APPARATUS AND METHOD 有权用于检测目标物质的装置和方法或用于这些装置和方法的装置公开(公告)号：US20090079988A1
公开(公告)日：2009-03-26
申请号：US12233184
申请日：2008-09-18
CPC分类号： G01N21/648 , G01N21/6428
摘要： An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a martial other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.
摘要翻译： 本发明的目的是检测具有高对比度的目标物质。 本发明涉及使用透光基板和用于诱导等离子体共振的金属的目标物质的分析，并且还使用具有与基板形成界面的开口部分的低折射率层，并且具有较低的折射率 指数比底物。 从基板侧发射的光在界面处被全反射，以照射具有ev逝光的开口部中的金属。 检测由目标物质通过ev逝光的等离子体共振产生的光。 根据本发明，可以减少对目标物质以外的其他武器的ev逝光的照射，从而可以减少来自与目标物质以外的武装物（例如，悬浮在目标物质周围的分子）的发光。
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16.发明授权Photosensitive resin composition, and multilayer printed circuit board using the same 失效感光树脂组合物和使用其的多层印刷电路板公开(公告)号：US06190834B1
公开(公告)日：2001-02-20
申请号：US09073645
申请日：1998-05-06
申请人： Masatoshi Narahara , Mineo Kawamoto , Tokihito Suwa , Masao Suzuki , Satoru Amou , Akio Takahashi , Hiroyuki Fukai , Mitsuo Yokota , Shiro Kobayashi , Masashi Miyazaki
发明人： Masatoshi Narahara , Mineo Kawamoto , Tokihito Suwa , Masao Suzuki , Satoru Amou , Akio Takahashi , Hiroyuki Fukai , Mitsuo Yokota , Shiro Kobayashi , Masashi Miyazaki
IPC分类号： G03C173
CPC分类号： C08F290/144 , C08F290/14 , G03F7/0388 , H01L2224/16225 , H05K3/0023 , H05K3/4661 , H05K2201/0133 , Y10S522/905
摘要： The present invention provides a photosensitive resin composition capable of forming an insulating film, which is superior in both a roughening property and an adhesiveness, and a via-hole, which is highly reliable in connection, and a multilayer printed circuit board. The present invention provides a photosensitive resin composition containing a first resin, which is an epoxy resin, and a second resin having a N-substituted carbamic acid ester atomic group and a radical polymeric unsaturated bond in its side chain. The second resin is desirably an oligomer having a repeating unit expressed by the following general formula (chem. 1) or (chem. 3) by 3-10 units. Where, X is H or CH3, Y and Z is H or an alkyl group of carbon number 1-4, n is 0 or 1, a part of R1 is an atomic group expressed by the following general formula (chem. 2), the residual R1 is a hydroxyl group, and R2 is an alkylene group of carbon number 1-4.
摘要翻译： 本发明提供能够形成绝缘膜的感光性树脂组合物，其具有优异的粗糙性和粘合性，以及连接高度可靠的通孔以及多层印刷电路板。本发明 提供含有作为环氧树脂的第一树脂和在其侧链具有N-取代氨基甲酸酯原子团和自由基聚合不饱和键的第二树脂的感光性树脂组合物。 第二树脂理想地是具有由以下通式（化学式1）或（化学式3）表示的重复单元的低聚物3-10单元。 其中，X为H或CH3，Y和Z为H或碳数为1-4的烷基，n为0或1，R1的一部分为由以下通式（化学式2）表示的原子团， 残基R1为羟基，R2为碳数为1-4的亚烷基。
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