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    Full-Length Infectious Cdna Clone for Porcine Reproductive and Respiratory Syndrome Virus(Prrsv) and Uses Thereof
    14.
    发明申请
    Full-Length Infectious Cdna Clone for Porcine Reproductive and Respiratory Syndrome Virus(Prrsv) and Uses Thereof 审中-公开
    猪繁殖与呼吸综合征病毒（Prrsv）全长感染性Cdna克隆及其用途

    公开(公告)号：US20080131459A1

    公开(公告)日：2008-06-05

    申请号：US11571878

    申请日：2005-07-09

    申请人： Seung Han Lee ,  Young-Min Lee

    发明人： Seung Han Lee ,  Young-Min Lee

    IPC分类号： A61K39/12 ,  C07H21/02 ,  C12N15/00 ,  C12N5/06 ,  C12Q1/68 ,  A61K48/00 ,  C07H21/04 ,  C12N7/00

    CPC分类号： C07K14/005 ,  A61K2039/5256 ,  A61K2039/53 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/10021 ,  C12N2770/10022 ,  C12N2770/10043 ,  C12N2800/204 ,  C12N2840/203

    摘要： The present invention relates to a novel full-length infectious cDNA clone for Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), derivatives therefrom and uses thereof Particularly, the present invention relates to a full-length PRRSV genomic RNA represented by SEQ. ID. No 27 and the genetically stable full-length infectious PRRSV cDNA clone thereof. The PRRSV genomic RNA and infectious PRRSV cDNA clone of the present invention can be used not only for the identification of the PRRSV viral genes, but also for the molecular biological studies including viral replication, transcription and translation. Moreover, it can also be applied to the development of the therapeutic agents, vaccines, diagnostic reagents and diagnostic devices for porcine reproductive and respiratory syndrome and can be used as a novel expression vector for a variety of heterologous genes of interest.

    摘要翻译： 本发明涉及一种用于猪繁殖与呼吸综合征病毒（PRRSV）的新型全长感染性cDNA克隆及其衍生物及其用途。特别地，本发明涉及SEQ ID NO：1所示的全长PRRSV基因组RNA。 ID。 27号和遗传稳定的全长感染性PRRSV cDNA克隆。 本发明的PRRSV基因组RNA和感染性PRRSV cDNA克隆不仅可用于鉴定PRRSV病毒基因，而且可用于分子生物学研究，包括病毒复制，转录和翻译。 此外，它还可以用于开发用于猪繁殖和呼吸综合征的治疗剂，疫苗，诊断试剂和诊断装置，并且可以用作各种感兴趣的异源基因的新型表达载体。

    PRRSV vaccines
    15.
    发明申请
    PRRSV vaccines 审中-公开
    PRRSV疫苗

    公开(公告)号：US20030049274A1

    公开(公告)日：2003-03-13

    申请号：US09948747

    申请日：2001-09-07

    发明人： Janneke Meulenberg ,  Helene Verheije

    IPC分类号： C12Q001/70 ,  G01N033/53 ,  C07H021/04 ,  C12P021/06 ,  C12P019/34 ,  A61K039/12 ,  A61K039/21 ,  A61K039/155

    CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K2039/51 ,  A61K2039/5256 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/10022 ,  C12N2770/10034 ,  C12N2770/10043 ,  C12N2770/10061

    摘要： The invention relates to the field of PRRS viruses and infectious clones obtained from PRRS viruses. Furthermore, the invention relates to vaccines and diagnostic assays obtainable by using and modifying such infectious clones of PRRS viruses. The invention provides a porcine reproductive and respiratory syndrom virus (PRRSV) replicon having at least some of its original PRRSV nucleic acid deleted, said replicon capable of in vivo RNA replication, said replicon further having been deprived of at least some of its original PRRSV nucleic acid and/or having been supplemented with nucleic acid derived from a heterologous microorganism.

    摘要翻译： 本发明涉及从PRRS病毒获得的PRRS病毒和感染性克隆的领域。 此外，本发明涉及通过使用和修饰PRRS病毒感染性克隆而获得的疫苗和诊断测定法。 本发明提供了具有其原始PRRSV核酸的至少一些缺失的猪繁殖和呼吸综合征病毒（PRRSV）复制子，所述复制子能够进行体内RNA复制，所述复制子还被剥夺了其原始PRRSV核酸的至少一些 酸和/或已经补充有源自异源微生物的核酸。

    Infectious cDNA clone of North American porcine reproductive and respiratory syndrome (PRRS) virus and uses thereof
    16.
    发明授权
    Infectious cDNA clone of North American porcine reproductive and respiratory syndrome (PRRS) virus and uses thereof 有权
    北美猪繁殖与呼吸综合征（PRRS）病毒的传染性cDNA克隆及其用途

    公开(公告)号：US06500662B1

    公开(公告)日：2002-12-31

    申请号：US09470661

    申请日：1999-12-22

    申请人： Jay G. Calvert ,  Michael G. Sheppard ,  Siao-Kun W. Welch

    发明人： Jay G. Calvert ,  Michael G. Sheppard ,  Siao-Kun W. Welch

    IPC分类号： C12N700

    CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K2039/525 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/552 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/10021 ,  C12N2770/10034 ,  C12N2770/10043 ,  C12N2770/10061 ,  C12N2770/10062

    摘要： The invention provides isolated polynucleotide molecules, including plasmids; viral vectors; and transfected host cells that comprise a DNA sequence encoding an infectious RNA sequence encoding a North American PRRS virus; and also North American PRRS viruses encoded thereby. The invention further provides isolated infectious RNA molecules encoding a North American PRRS virus. The invention also provides isolated polynucleotide molecules, infectious RNA molecules, viral vectors, and transfected host cells encoding genetically-modified North American PRRS viruses; and genetically-modified North American PRRS viruses encoded thereby. The invention also provides vaccines comprising such plasmids, RNA molecules, viral vectors, and North American PRRS viruses, and methods of using these vaccines in swine and in other animals. Also provided are isolated polynucleotide molecules, viral vectors, and transfected host cells that comprise a nucleotide sequence encoding a peptide of a North American PRRS virus. These viral vectors and transfected host cell lines are useful in providing peptides to compensate for mutated peptide coding sequences of DNA sequences encoding genetically-modified North American PRRS viruses so that functional virions can be generated.

    摘要翻译： 本发明提供分离的多核苷酸分子，包括质粒; 病毒载体; 和包含编码编码北美PRRS病毒的感染性RNA序列的DNA序列的转染宿主细胞; 以及由此编码的北美PRRS病毒。 本发明还提供编码北美PRRS病毒的分离的感染性RNA分子。 本发明还提供分离的多核苷酸分子，感染性RNA分子，病毒载体和编码经遗传修饰的北美PRRS病毒的转染宿主细胞; 和由此编码的转基因北美PRRS病毒。 本发明还提供包含这样的质粒，RNA分子，病毒载体和北美PRRS病毒的疫苗，以及在猪和其他动物中使用这些疫苗的方法。 还提供了分离的多核苷酸分子，病毒载体和包含编码北美PRRS病毒的肽的核苷酸序列的转染宿主细胞。 这些病毒载体和转染的宿主细胞系可用于提供肽以补偿编码经遗传修饰的北美PRRS病毒的DNA序列的突变的肽编码序列，从而可以产生功能性病毒体。