公开(公告)号：US20220348932A1

公开(公告)日：2022-11-03

申请号：US17516424

申请日：2021-11-01

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N15/52 ,  C12N9/88 ,  C12N9/90 ,  C12N9/04 ,  C12N9/10 ,  C12P7/04

Abstract: The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

公开(公告)号：US11447804B2

公开(公告)日：2022-09-20

申请号：US16178432

申请日：2018-11-01

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P17/10 ,  C12P7/44 ,  C12P7/24 ,  C12P13/00 ,  C12N9/04 ,  C08G63/78 ,  C08G73/02 ,  C08G69/16 ,  C08G69/08 ,  A23L29/00 ,  C12N15/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

公开(公告)号：US11434512B2

公开(公告)日：2022-09-06

申请号：US16234315

申请日：2018-12-27

Applicant: GENOMATICA, INC.

Inventor： Fernando Sanchez-Riera ,  Stephen Del Cardayre ,  Fernando Valle

IPC: C12P7/64 ,  C12P7/06 ,  C12N1/21 ,  C12N1/19 ,  C12N9/18 ,  C12N9/00 ,  C12N9/10 ,  C12P7/649 ,  C12N9/02 ,  C12N9/52 ,  C12N9/04

Abstract: Methods of producing fatty acid esters, such as fatty acid ethyl esters, from genetically engineered microorganisms are described.

公开(公告)号：US11421206B2

公开(公告)日：2022-08-23

申请号：US16661667

申请日：2019-10-23

Applicant: Genomatica, Inc.

Inventor： Baolong Zhu ,  Andreas W. Schirmer ,  Cindy Chang

IPC: C12N9/02 ,  C12N15/62 ,  C12P7/6409

Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.

公开(公告)号：US20220235385A1

公开(公告)日：2022-07-28

申请号：US17605196

申请日：2020-04-24

Applicant: Genomatica, Inc.

Inventor： Amit M. Shah ,  Harish Nagarajan

IPC: C12P13/00 ,  C12P17/08 ,  C12P17/10 ,  C12P7/18 ,  C12P7/44 ,  C12P7/62 ,  C12N9/02

Abstract: Disclosed are biosynthetic methods and engineered microorganism that enhance or improve the biosynthesis of hexamethylenediamine, caproic acid or caprolactam. The engineered microorganisms include selected aldehyde dehydrogenase activity.

公开(公告)号：US11384340B2

公开(公告)日：2022-07-12

申请号：US16792592

申请日：2020-02-17

Applicant: Genomatica, Inc.

Inventor： Stefan Andrae ,  Michael Patrick Kuchinskas ,  Jingyi Li ,  Harish Nagarajan ,  Priti Pharkya

IPC: C12N9/04 ,  C12P7/24 ,  C12P7/18

Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

公开(公告)号：US20220177858A1

公开(公告)日：2022-06-09

申请号：US17604722

申请日：2020-04-17

Applicant: Genomatica, Inc.

Inventor： Michael A. Noble ,  Russell Scott Komor ,  Jingyi Li

IPC: C12N9/10 ,  C12N15/52 ,  C12N1/20 ,  C12P7/22

Abstract: Described herein are non-natural olivetol synthase (OLS) variants, nucleic acids, engineered cells, method s for preparing cannabinoids, and compositions thereof. The non-natural olivetol OLS variants form desired cannabinoid precursor and products at increased rates, have higher affinity for pathway substrates, and/or byproducts are formed in lower amounts in their presence, as compared to wild type OLS. The OLS variants can be used to form linear polyketides, and can be expressed in an engineered cell having a pathway to form cannabinoids, which include CBGA, its analogs and derivatives. CBGA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.

公开(公告)号：US20220098625A1

公开(公告)日：2022-03-31

申请号：US17426973

申请日：2020-01-30

Applicant: Genomatica, Inc.

Inventor： Lauri H. Suominen ,  Jefferson Lievense ,  Aiguo Liu ,  Jorge Jesus Martinez-Gonzalez ,  Myong Kon Ko ,  Wei Nan

IPC: C12P7/22 ,  B01D11/02 ,  C12M1/26

Abstract: Methods of recovering cannabinoids from cell cultures include methods comprising steps of separating the cell culture at a temperature above the melting point of the cannabinoid to separate a light phase comprising liquid state cannabinoid from a heavy phase; and methods comprising treating the cell culture at a temperature below the melting point of the cannabinoid to separate a light phase from a heavy phase comprising solid state cannabinoid. Other methods include contacting the culture with a water-miscible solvent to form a water-miscible phase and an aqueous phase, separating the two phases and recovering the cannabinoid. Other methods include contacting the culture with a water-immiscible solvent to form a water-immiscible phase and an aqueous phase, separating the two phases, and recovering the cannabinoid. Other methods include washing the inner surface of a fermentation vessel with alkaline solution to recover cannabinoid attached to the vessel surface. Various methods make use of aqueous solvent systems comprising no organic solvent, aqueous solvent systems comprising added water-miscible organic solvent, and dual-phase aqueous/water-immiscible solvent systems.

公开(公告)号：US20220002356A1

公开(公告)日：2022-01-06

申请号：US17271442

申请日：2019-08-29

Applicant: Genomatica, Inc.

Inventor： Stephen Thomas PAYNE ,  Scott Allen FRYKMAN ,  Bernardo Moura Torres DA COSTA ,  Isolde CALLIHAN ,  Sankaranarayanan VENKITESWARAN ,  Leland Ken WONG

IPC: C07K14/245 ,  C12P7/64 ,  C12N1/22 ,  C12N9/02 ,  C12N9/06 ,  C12N9/10 ,  C12N9/18

Abstract: The disclosure relates to mutant gene(s) that confer upon microorganisms that express them an improved capacity to utilize xylose and improved capacity to co-utilize glucose and xylose thereby resulting in improved growth of the microorganism. Further encompassed are methods of producing fatty acids and fatty acid derivatives from cellulosic biomass, xylose, and/or a glucose/xylose mix by employing the host cells expressing the engineered XylR variants and compositions of biologically produced fatty acids and fatty acid derivatives.

公开(公告)号：US20210371836A1

公开(公告)日：2021-12-02

申请号：US17204449

申请日：2021-03-17

Applicant: Genomatica, Inc.

Inventor： Louis HOM ,  Na TRINH ,  Murtaza ALIBHAI ,  Zhihao HU ,  Eli GROBAN ,  Vikranth ARLAGADDA ,  Elizabeth CLARKE

IPC: C12N9/16 ,  C12N9/18 ,  C12N15/00 ,  C12N15/63

Abstract: The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.