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21.
公开(公告)号:US07456014B2
公开(公告)日:2008-11-25
申请号:US11476510
申请日:2006-06-28
申请人: Anthony J. Kinney , Kevin L. Stecca
发明人: Anthony J. Kinney , Kevin L. Stecca
CPC分类号: C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8246 , C12N15/8247 , C12N15/825 , C12N15/8251
摘要: Recombinant constructs useful for reducing the expression of endogenous mRNA and any substantially similar endogenous mRNA are disclosed. In particular, a recombinant construct comprising, inter alia, a suitable nucleic acid sequence and its reverse complement can be used to alter the expression of any homologous, endogenous RNA (i.e., the target RNA) which is in proximity to this suitable nucleic acid sequence.
摘要翻译: 公开了可用于减少内源性mRNA的表达和任何基本上相似的内源mRNA的重组构建体。 特别地,包含尤其是合适的核酸序列及其反向互补序列的重组构建体可用于改变任何同源的内源RNA(即靶RNA)的表达,其接近该合适的核酸序列 。
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公开(公告)号:US20080209596A1
公开(公告)日:2008-08-28
申请号:US11970805
申请日:2008-01-08
CPC分类号: C07K14/415 , C12N15/8245
摘要: This invention relates to an isolated nucleic acid fragment encoding a sugar transport protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sugar transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sugar transport protein in a transformed host cell.
摘要翻译: 本发明涉及编码糖转运蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分糖转运蛋白的嵌合基因,其中嵌合基因的表达导致转化的宿主细胞中糖转运蛋白的水平的改变 。
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公开(公告)号:US07365242B2
公开(公告)日:2008-04-29
申请号:US10757155
申请日:2004-01-14
申请人: Anthony J. Kinney , Gary M. Fader
发明人: Anthony J. Kinney , Gary M. Fader
CPC分类号: C12N15/8216 , C07K14/415 , C12N15/8218 , C12N15/8247 , C12N15/8251
摘要: This invention concerns the construction of transgenic soybean lines wherein the expression of genes encoding seed storage proteins are modulated to effect a change in seed storage protein profile of transgenic plants. Modification of the seed storage protein profile can result in the production of novel soy protein products with unique and valuable functional characteristics.
摘要翻译: 本发明涉及转基因大豆品系的构建,其中编码种子储存蛋白的基因的表达被调节以影响转基因植物的种子储存蛋白谱的变化。 种子储存蛋白质谱的修饰可以产生具有独特且有价值的功能特征的新型大豆蛋白产品。
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公开(公告)号:US07157621B2
公开(公告)日:2007-01-02
申请号:US10183687
申请日:2002-06-27
申请人: Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
发明人: Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
CPC分类号: C12N15/8247 , C07K14/415
摘要: The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.
摘要翻译: 公开了可用于改变植物油表型的核酸片段的制备和使用。 掺入此类核酸片段和合适调节序列的嵌合构建体可用于产生具有改变的脂质分布的转基因植物。 还公开了使用这种核酸片段改变植物油表型的方法。
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公开(公告)号:US06900369B2
公开(公告)日:2005-05-31
申请号:US10233926
申请日:2002-09-03
申请人: Stephen M. Allen , Karlene H. Butler , Saverio Carl Falco , Anthony J. Kinney , Kevin L. Stecca
发明人: Stephen M. Allen , Karlene H. Butler , Saverio Carl Falco , Anthony J. Kinney , Kevin L. Stecca
CPC分类号: C12N9/1241 , C12N15/8243
摘要: This invention relates to an isolated nucleic acid fragment encoding a cholinephosphate cytidylyltransferase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the cholinephosphate cytidylyltransferase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the cholinephosphate cytidylyltransferase in a transformed host cell.
摘要翻译: 本发明涉及编码胆碱磷酸胞苷酰转移酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分胆碱磷酸胞苷酰基转移酶的嵌合基因,其中嵌合基因的表达导致转化宿主细胞中胆碱磷酸胞苷酰转移酶水平的改变。
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公开(公告)号:US06864077B1
公开(公告)日:2005-03-08
申请号:US09857524
申请日:1999-12-02
CPC分类号: C12N9/0083
摘要: This invention relates to an isolated nucleic acid fragment encoding a delta-6 desaturase or sphingolipid desaturase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the delta-6 desaturase or sphingolipid desaturase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the delta-6 desaturase or sphingolipid desaturase in a transformed host cell.
摘要翻译: 本发明涉及编码δ-6去饱和酶或鞘脂脱氢酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码Δ-6去饱和酶或鞘脂脱氢酶的全部或部分的嵌合基因,其中嵌合基因的表达导致产生改变的δ-6去饱和酶水平 或鞘脂脱氢酶在转化的宿主细胞中。
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公开(公告)号:US06849783B2
公开(公告)日:2005-02-01
申请号:US09740288
申请日:2000-12-19
CPC分类号: C12N15/52
摘要: This invention relates to an isolated nucleic acid fragment encoding a biotin synthases. The invention also relates to the construction of a chimeric gene encoding all or a portion of the biotin synthases, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the biotin synthases in a transformed host cell.
摘要翻译: 本发明涉及编码生物素合酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或一部分生物素合酶的嵌合基因,其中嵌合基因的表达导致转化宿主细胞中生物素合酶水平的改变。
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公开(公告)号:US06838594B1
公开(公告)日:2005-01-04
申请号:US09664840
申请日:2000-09-19
CPC分类号: C07H21/04 , C12N9/0083 , C12N9/1029 , C12N15/8247
摘要: This invention relates to an isolated nucleic acid fragment encoding an enzyme involved in lipid biosynthesis. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme involved in lipid biosynthesis, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme involved in lipid biosynthesis in a transformed host cell.
摘要翻译: 本发明涉及编码参与脂质生物合成的酶的分离的核酸片段。 本发明还涉及在正义或反义方向上编码参与脂质生物合成的全部或部分酶的嵌合基因的构建,其中嵌合基因的表达导致生产参与脂质生物合成的酶水平的变化 转化的宿主细胞。
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公开(公告)号:US06570064B1
公开(公告)日:2003-05-27
申请号:US09645168
申请日:2000-08-24
IPC分类号: A01H300
CPC分类号: C12N9/0071 , C12N15/8243 , C12N15/825 , C12Q1/26 , G01N2500/00
摘要: This invention relates to an isolated nucleic acid fragment encoding a flavanone-3-hydroxylase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the flavanone-3-hydroxylase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the flavanone-3-hydroxylase in a transformed host cell.
摘要翻译: 本发明涉及编码黄烷酮-3-羟化酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分黄烷酮-3-羟化酶的嵌合基因,其中嵌合基因的表达导致黄酮酮-3-羟化酶水平的改变在 转化的宿主细胞。
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公开(公告)号:US06451581B1
公开(公告)日:2002-09-17
申请号:US09173300
申请日:1998-10-15
申请人: Saverio Carl Falco , Rebecca E. Cahoon , William D. Hitz , Anthony J. Kinney , J. Antoni Rafalski
发明人: Saverio Carl Falco , Rebecca E. Cahoon , William D. Hitz , Anthony J. Kinney , J. Antoni Rafalski
IPC分类号: C12N120
CPC分类号: C12N15/8251 , C12N9/1096 , C12N9/88 , Y10S435/948
摘要: This invention relates to an isolated nucleic acid fragment encoding a branched-chain biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the branched-chain biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the branched-chain biosynthetic enzyme in a transformed host cell.
摘要翻译: 本发明涉及编码支链生物合成酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分支链生物合成酶的嵌合基因,其中嵌合基因的表达导致支链生物合成酶水平的改变在 转化的宿主细胞。
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