公开(公告)号：US5998796A

公开(公告)日：1999-12-07

申请号：US84236

申请日：1998-05-26

Applicant: Changsheng Liu ,  Qingbo Li

Inventor： Changsheng Liu ,  Qingbo Li

IPC: G01N21/64 ,  G01N27/447

CPC classification number: G01N27/44721

Abstract: A detector for DNA sample identification is provided with a transmission grating beam splitter (TGBS). The TGBS split fluoresced light from a tagged DNA sample into 0th order and a 1st order components, both of which are detected on a two-dimensional detector array of a CCD camera. The 0th and 1st order components are detected along a column of pixels in the detector array, and are spaced apart from one another. The DNA samples are tagged with four fluorescent dyes, one dye specific for each nucleotide, and all four dyes responding in slightly different manner to the same monochromatic excitation signal. The TGBS splits fluoresced incoming light into 0th and 1st order components, which are then spread out among a number of pixels in the detector array. The 1st component of this light is received by pixels whose position relative to the 0th order component depends on the frequency of fluorescence. Thus, the position at which signal energy is detected on the array is indicative of the particular dye, and therefore, the corresponding nucleotide tagged by that dye. Monitoring signal energy at the 0th order pixel and selected 1st order pixels, provides a set of data from which one may then identify the particular nucleotide.

公开(公告)号：US08086410B1

公开(公告)日：2011-12-27

申请号：US12117911

申请日：2008-05-09

Applicant: Changsheng Liu ,  Fei Gao

Inventor： Changsheng Liu ,  Fei Gao

IPC: G06F19/00 ,  C12Q1/68

CPC classification number: G06F19/18 ,  G06F19/22

Abstract: A method for detecting DNA variation. First, by aligning trace data of a sample DNA sequence to trace data of a reference DNA sequence to produce an aligned sample DNA sequence. Then, inputting the trace data of the bases of both the reference DNA sequence and the aligned sample DNA sequence for a particular frame number into a non-linear mathematical function of an anti-correlation calculation scheme for all the frame numbers. Minimal values will be produced at the particular frame number for DNA base trace data of the aligned sample DNA sequence which are not a variation as compared to the reference DNA sequence. Values above the minimal values will be produced at the particular frame number for DNA base trace data of the aligned sample DNA sequence which are a variation as compared to the reference DNA sequence.

Abstract translation: 检测DNA变异的方法。 首先，通过将样本DNA序列的痕量数据对齐到追踪参考DNA序列的数据以产生排列的样品DNA序列。 然后，将用于特定帧号的参考DNA序列和对准样品DNA序列的碱基的痕迹数据输入到所有帧号的反相关计算方案的非线性数学函数中。 与对照样品DNA序列的DNA碱基痕量数据的特定帧编号产生最小值，与参考DNA序列相比不是变异。 高于最小值的值将在对准的样品DNA序列的DNA碱基痕量数据的特定帧数产生，这是与参考DNA序列相比的变体。

公开(公告)号：US20100116659A1

公开(公告)日：2010-05-13

申请号：US12495513

申请日：2009-06-30

Applicant: ChangSheng Liu ,  Kevin Le Van

Inventor： ChangSheng Liu ,  Kevin Le Van

IPC: B01D57/02 ,  G01N27/00

CPC classification number: G01N27/44782 ,  G01N27/44721 ,  G01N27/44743 ,  G01N27/44773

Abstract: The present invention relates to a method for separating components of a sample. The method includes obtaining a first separation of the sample components along a first dimension wherein the sample components are at least partially resolved, wherein the first separation can be performed in the absence of an electric field applied to the first dimension. An electric field is used to obtain a second separation of the sample components along a second dimension comprising a plurality of substantially isolated volumes. An intensity-time data record is obtained from each of the isolated volumes, the intensity-time data records containing peaks, each peak being indicative of a migration time. The migration time of a first peak is normalized with respect to a migration time of at least a second peak to correct for migration time differences between the isolated volumes.

Abstract translation: 本发明涉及一种分离样品组分的方法。 该方法包括沿第一维度获得样品组分的第一分离，其中样品组分至少部分被分离，其中第一分离可以在没有施加到第一维度的电场的情况下进行。 电场用于沿着包括多个基本上隔离的体积的第二维度获得样品组分的第二分离。 从每个孤立的体积获得强度时间数据记录，强度 - 时间数据记录包含峰值，每个峰值表示迁移时间。 相对于至少第二峰的迁移时间，第一峰的迁移时间被归一化以校正隔离体积之间的迁移时间差。

公开(公告)号：US20090301884A1

公开(公告)日：2009-12-10

申请号：US12154598

申请日：2008-05-23

Applicant: Changsheng Liu ,  Hequan Zhao

Inventor： Changsheng Liu ,  Hequan Zhao

IPC: C07K1/26

CPC classification number: G06K9/00127 ,  G01N27/44721

Abstract: An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

Abstract translation: 具有多个分离通道的电泳系统设置有自动校准特征，其中每个通道被单独校准。 对于每个通道，校准系数将接收的通道强度的光谱映射到反映存在多种染料中的每一种的相对似然性的值。 反映单一染料的影响的各个峰从各种检测的光强度谱组中分离出来，并且这些峰可用于检测存在的染料成分的数量，并且还可以建立用于校准系数的示例性载体 然后可以将其聚类并进一步处理以得到系统的校准矩阵。 因此，该系统允许使用不同的染料组来标记在单独泳道中迁移的样品中的DNA核苷酸，并且还允许用新的先前未使用的染料组进行原位校准。

公开(公告)号：US07329333B2

公开(公告)日：2008-02-12

申请号：US10679514

申请日：2003-10-07

Applicant: Qingbo Li ,  Songsan Zhou ,  Changsheng Liu

Inventor： Qingbo Li ,  Songsan Zhou ,  Changsheng Liu

IPC: G01N27/26

CPC classification number: G01N27/44721 ,  G01N27/44782

Abstract: A capillary electrophoresis system comprises capillaries positioned in parallel to each other forming a plane. The capillaries are configured to allow samples to migrate. A light source is configured to illuminate the capillaries and the samples therein. This causes the samples to emit light. A lens is configured to receive the light emitted by the samples and positioned directly over a first group of the capillaries and obliquely over a second group of the capillaries. The light source is further configured to illuminate the second group of capillaries more than the first group of the capillaries such that amount of light received by the lens from the first group of capillaries is substantially identical to amount of light received from the second group of capillaries when an identical amount of the samples is migrating through the first and second group capillaries.

Abstract translation: 毛细管电泳系统包括彼此平行地形成平面的毛细管。 毛细管被配置为允许样品迁移。 光源被配置为照亮毛细管和其中的样品。 这样会使样品发光。 透镜被配置为接收由样品发射的光并且直接定位在第一组毛细管上并且倾斜地位于第二组毛细管上。 光源还被配置为比第一组毛细管照亮第二组毛细管，使得透镜从第一组毛细管接收的光量与从第二组毛细血管接收的光量基本相同 当相同量的样品通过第一和第二组毛细管迁移时。

公开(公告)号：US07094286B2

公开(公告)日：2006-08-22

申请号：US10468648

申请日：2001-02-26

Applicant: Changsheng Liu

Inventor： Changsheng Liu

IPC: A61L24/02

CPC classification number: G03G15/09 ,  A61L24/02 ,  A61L2430/02 ,  B82Y15/00 ,  G03G2221/183 ,  Y10S623/923

Abstract: The present invention discloses an inorganic bone adhesive and its use in human hard tissue repairs. The inorganic bone adhesive comprises basic compound, phosphate, calcium phosphate bone cement and retarder with the characteristics of rapid hydration rate and high early strength. Inorganic Bone adhesive can be widely used in the artificial joints fixation, screw fixation as well as comminuted fracture fixation. It is a kind of safe and effective adhesive material and beneficial for the fast postoperative recovery. The final hydration reaction products contains the composition of magnesium phosphate, bio-mineral containing ammonium and apatite-like materials, which has excellent biocompatibility and can be gradually absorbed by surrounding tissues after being implanted in vivo, which benefits the in-growth of the new bone.

Abstract translation: 本发明公开了一种无机骨粘合剂及其在人体硬组织修复中的应用。 无机骨粘合剂包括碱性化合物，磷酸盐，磷酸钙骨水泥和缓凝剂，具有水化速度快，早期强度高的特点。 无机骨粘合剂可广泛用于人造关节固定，螺钉固定以及粉碎骨折固定。 它是一种安全有效的粘合剂材料，有利于术后快速恢复。 最终的水化反应产物含有磷酸镁，含有矿物质和磷灰石的物质的生物矿物质组成，具有极好的生物相容性，植物体内可被周围组织逐渐吸收，有利于新生物的生长 骨。

公开(公告)号：US06974527B2

公开(公告)日：2005-12-13

申请号：US09874331

申请日：2001-06-06

Applicant: ChangSheng Liu ,  Kevin Le Van

Inventor： ChangSheng Liu ,  Kevin Le Van

IPC: G01N27/447 ,  G01N27/453 ,  G01N30/02

CPC classification number: G01N27/44782 ,  G01N27/44721 ,  G01N27/44743 ,  G01N27/44773

Abstract: The present invention relates to a method for separating components of a sample. The method includes obtaining a first separation of the sample components along a first dimension wherein the sample components are at least partially resolved, wherein the first separation can be performed in the absence of an electric field applied to the first dimension. An electric field is used to obtain a second separation of the sample components along a second dimension comprising a plurality of substantially isolated volumes. An intensity-time data record is obtained from each of the isolated volumes, the intensity-time data records containing peaks, each peak being indicative of a migration time. The migration time of a first peak is normalized with respect to a migration time of at least a second peak to correct for migration time differences between the isolated volumes.

公开(公告)号：US06953521B2

公开(公告)日：2005-10-11

申请号：US10011977

申请日：2001-12-11

Applicant: Changsheng Liu ,  Thomas E Kane ,  Qingbo Li

Inventor： Changsheng Liu ,  Thomas E Kane ,  Qingbo Li

IPC: G01N27/447 ,  G01N27/453

CPC classification number: C07K1/26 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  Y10T436/2575

Abstract: An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresced into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

Abstract translation: 公开了一种自动化毛细管区域电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有连接到毛细管的检测端的容器。 该容器设置有阀，其有助于清洁毛细管，将缓冲液加载到毛细管中，将待电泳的样品引入毛细管中，并在这样引入的样品上进行毛细管区带电泳。

公开(公告)号：US20050217996A1

公开(公告)日：2005-10-06

申请号：US11104327

申请日：2005-04-12

Applicant: ChangSheng Liu ,  Kevin Le Van

Inventor： ChangSheng Liu ,  Kevin Le Van

IPC: G01N27/447 ,  G01N27/453

CPC classification number: G01N27/44782 ,  G01N27/44721 ,  G01N27/44743 ,  G01N27/44773

Abstract: The present invention relates to a method for separating components of a sample. The method includes obtaining a first separation of the sample components along a first dimension wherein the sample components are at least partially resolved, wherein the first separation can be performed in the absence of an electric field applied to the first dimension. An electric field is used to obtain a second separation of the sample components along a second dimension comprising a plurality of substantially isolated volumes. An intensity-time data record is obtained from each of the isolated volumes, the intensity-time data records containing peaks, each peak being indicative of a migration time. The migration time of a first peak is normalized with respect to a migration time of at least a second peak to correct for migration time differences between the isolated volumes.

Abstract translation: 本发明涉及一种分离样品组分的方法。 该方法包括沿第一维度获得样品组分的第一分离，其中样品组分至少部分被分离，其中第一分离可以在没有施加到第一维度的电场的情况下进行。 电场用于沿着包括多个基本上隔离的体积的第二维度获得样品组分的第二分离。 从每个孤立的体积获得强度时间数据记录，强度 - 时间数据记录包含峰值，每个峰值表示迁移时间。 相对于至少第二峰的迁移时间，第一峰的迁移时间被归一化以校正隔离体积之间的迁移时间差。

公开(公告)号：US06929729B2

公开(公告)日：2005-08-16

申请号：US09757632

申请日：2001-01-11

Applicant: ChangSheng Liu ,  Kevin J. Levan

Inventor： ChangSheng Liu ,  Kevin J. Levan

IPC: G01N27/447 ,  G01N27/26 ,  B01D57/02

CPC classification number: G01N27/44747 ,  G01N27/44704 ,  G01N27/44743

Abstract: A method for conducting capillary zone electrophoresis in which a lubricating detergent is added to help prevent sample buildup on the inner walls of the capillary. In a preferred embodiment the lubricating detergent is sodium dodecylsulfate (SDS) in a concentration of about 3 mM added to a protein sample before the sample is introduced into one end of a capillary. The SDS may be added to the buffer, instead of the sample, before electrophoresis.

Abstract translation: 一种用于进行毛细管区带电泳的方法，其中加入润滑洗涤剂以帮助防止毛细管内壁上的样品积聚。 在优选的实施方案中，将样品引入毛细管的一端之前，将蛋白质样品中的浓度为约3mM的润滑洗涤剂加入到十二烷基硫酸钠（SDS）中。 在电泳之前，可以将SDS加入到缓冲液中而不是样品中。