公开(公告)号：US20220112508A1

公开(公告)日：2022-04-14

申请号：US17555395

申请日：2021-12-18

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12N15/68 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20220112452A1

公开(公告)日：2022-04-14

申请号：US17555336

申请日：2021-12-17

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20220106589A1

公开(公告)日：2022-04-07

申请号：US17492435

申请日：2021-10-01

Applicant: Inscripta, Inc.

Inventor： Andrea HALWEG-EDWARDS ,  Thomas HRAHA ,  Krishna YERRAMSETTY ,  Shea LAMBERT ,  Miles GANDER ,  Matthew David ESTES ,  Chad Douglas SANADA ,  Isaac David WAGNER ,  Paul HARDENBOL

IPC: C12N15/10 ,  G16B35/10 ,  G16B40/20 ,  G16B35/20

Abstract: Disclosed systems and methods relate to predicting the relative representation of genomic variants in an edited cell population, based on the editing cassette design representation in an editing cassette design library used to generate the edited cell population. A library of editing cassette designs is generated, and a feature vector, or sequence embedding, is developed for each design using natural language processing techniques. The feature vector may be based upon sequence attributes and editing kinetics of each cassette design as well as attributes that describe the library context. Features may include sequence embeddings generated from a neural network, linguistic-type distances, and statistical distance summaries thereof. The feature vectors are classified using one or more machine learning models, and the classified feature vectors are used to predict the representation of each design an edited cell population.

公开(公告)号：US20220098562A1

公开(公告)日：2022-03-31

申请号：US17551391

申请日：2021-12-15

Applicant: Inscripta, Inc.

Inventor： Juhan Kim ,  Benjamin Mijts

IPC: C12N9/22

Abstract: The present disclosure provides a novel catalytically inactive MAD7 nuclease (dMAD7) that retains the ability to bind DNA in a sequence-specific manner. The MAD7 nuclease from which the dMAD7 has been derived was isolated from Eubacterium rectale.

公开(公告)号：US20220081693A1

公开(公告)日：2022-03-17

申请号：US17536067

申请日：2021-11-28

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome

公开(公告)号：US20220081687A1

公开(公告)日：2022-03-17

申请号：US17538922

申请日：2021-11-30

Applicant: Inscripta, Inc.

Inventor： Jorge Bernate ,  Kevin Ness ,  Phillip Belgrader ,  Don Masquelier ,  Ryan Gill

IPC: C12N15/10 ,  C40B40/02 ,  C12M1/26 ,  C12M1/42 ,  C12M1/34 ,  C12M3/00 ,  C12M1/12 ,  C12M1/36 ,  C12M1/00 ,  C12N9/22 ,  C12N15/11

Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

公开(公告)号：US20210388391A1

公开(公告)日：2021-12-16

申请号：US17387860

申请日：2021-07-28

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20210371799A1

公开(公告)日：2021-12-02

申请号：US17401362

申请日：2021-08-13

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Michael Graige ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M3/00 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US20210332389A1

公开(公告)日：2021-10-28

申请号：US17239540

申请日：2021-04-23

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Nathan Bade ,  Christian Siltanen ,  Aamir Mir ,  Xi-Jun Chen ,  Janine Mok ,  Burak Dura ,  Bruce Chabansky ,  David Stumbo ,  Eric Smith ,  Jorge Bernate

IPC: C12N15/90 ,  C12N15/10 ,  C12N5/074 ,  C12M1/00 ,  C12M3/00 ,  C12N15/88 ,  C12M3/04 ,  C12N15/11 ,  C12N9/22

Abstract: This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells.

公开(公告)号：US20210332388A1

公开(公告)日：2021-10-28

申请号：US17237747

申请日：2021-04-22

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Christian Siltanen ,  Burak Dura ,  Bruce Chabansky ,  David Stumbo ,  Eric Smith ,  Jorge Bernate

IPC: C12N15/90 ,  C12N15/10 ,  C12M3/06 ,  C12M1/00 ,  C12M1/32 ,  B01L7/00 ,  C12N15/11 ,  C12N9/22 ,  B01L3/00

Abstract: This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells.