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    Preparation of high purity phenothiazine N-alkylsulfonates and their use in chemiluminescent assays for the measurement of peroxidase activity
    21.
    发明授权
    Preparation of high purity phenothiazine N-alkylsulfonates and their use in chemiluminescent assays for the measurement of peroxidase activity 有权
    高纯度吩噻嗪N-烷基磺酸盐的制备及其在化学发光测定中用于测定过氧化物酶活性的用途

    公开(公告)号：US07855287B2

    公开(公告)日：2010-12-21

    申请号：US12010306

    申请日：2008-01-23

    申请人： Leopoldo Della Ciana

    发明人： Leopoldo Della Ciana

    IPC分类号： C07D279/26

    CPC分类号： C07D279/22 ,  G01N33/581 ,  G01N2333/908

    摘要： A process is described for preparing, efficiently and with a high degree of purity N-alkylsulfonates of phenothiazine. The process consists in (a) the preparation of the phenothiazine anion, and (b) the reaction of said anion with cyclic alkyl sulfonates, such as 1,3-propane sultone and 1,4-butane sultone. This process is simpler, more direct, and more efficient than the procedures currently used for the synthesis of N-alkylsulfonates derivatives of phenothiazine. In addition, the products obtained with this process are far more pure than those obtained through current procedures and therefore ideal for bioanalytical applications that require high sensitivity, such as assays based on the measurement of peroxidase activity using chemiluminescence.

    摘要翻译： 描述了制备，有效和高纯度吩噻嗪的N-烷基磺酸盐的方法。 该方法包括（a）吩噻嗪阴离子的制备，和（b）所述阴离子与环烷基磺酸盐如1,3-丙磺酸内酯和1,4-丁烷磺内酯的反应。 该方法比目前用于合成吩噻嗪的N-烷基磺酸盐衍生物的方法更简单，更直接且更有效。 此外，通过该方法获得的产品比通过当前方法获得的产品纯得多，因此对于需要高灵敏度的生物分析应用是理想的，例如基于使用化学发光测量过氧化物酶活性的测定法。

    Preparation of high purity phenothiazine N-alkylsulfonates and their use in chemiluminescent assays for the measurement of peroxidase acitivity
    22.
    发明申请
    Preparation of high purity phenothiazine N-alkylsulfonates and their use in chemiluminescent assays for the measurement of peroxidase acitivity 有权
    高纯度吩噻嗪N-烷基磺酸盐的制备及其在化学发光测定中用于测定过氧化物酶活性的用途

    公开(公告)号：US20080176251A1

    公开(公告)日：2008-07-24

    申请号：US12010306

    申请日：2008-01-23

    申请人： Leopoldo Della Ciana

    发明人： Leopoldo Della Ciana

    IPC分类号： G01N33/53 ,  C07D279/22 ,  C12Q1/28

    CPC分类号： C07D279/22 ,  G01N33/581 ,  G01N2333/908

    摘要： A process is described for preparing, efficiently and with a high degree of purity N-alkylsulfonates of phenothiazine. The process consists in (a) the preparation of the phenothiazine anion, and (b) the reaction of said anion with cyclic alkyl sulfonates, such as 1,3-propane sultone and 1,4-butane sultone. This process is simpler, more direct, and more efficient than the procedures currently used for the synthesis of N-alkylsulfonates derivatives of phenothiazine. In addition, the products obtained with this process are far more pure than those obtained through current procedures and therefore ideal for bioanalytical applications that require high sensitivity, such as assays based on the measurement of peroxidase activity using chemiluminescence.

    摘要翻译： 描述了制备，有效和高纯度吩噻嗪的N-烷基磺酸盐的方法。 该方法包括（a）吩噻嗪阴离子的制备，和（b）所述阴离子与环烷基磺酸盐如1,3-丙磺酸内酯和1,4-丁烷磺内酯的反应。 该方法比目前用于合成吩噻嗪的N-烷基磺酸酯衍生物的方法更简单，更直接且更有效。 此外，通过该方法获得的产品比通过当前方法获得的产品纯得多，因此对于需要高灵敏度的生物分析应用是理想的，例如基于使用化学发光测量过氧化物酶活性的测定法。

    Electrochemiluminescent assays
    23.
    发明授权
    Electrochemiluminescent assays 失效
    电化学发光测定

    公开(公告)号：US06916606B2

    公开(公告)日：2005-07-12

    申请号：US10274079

    申请日：2002-10-18

    申请人： Richard J. Massey ,  Michael J. Powell ,  Paul A. Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter J. Dressick ,  Mohindar S. Poonian

    发明人： Richard J. Massey ,  Michael J. Powell ,  Paul A. Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter J. Dressick ,  Mohindar S. Poonian

    IPC分类号： C07D473/08 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76 ,  C12Q1/70 ,  A61K38/00 ,  G01N33/53 ,  G01N33/566

    CPC分类号： G01N33/76 ,  C07D473/08 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582

    摘要： Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.Further provided are methods for detecting and identifying the presence of a multiplicity of analytes in a liquid food or food homogenate. These methods comprise immersing a diagnostic reagent holder, provided with a multiplicity of reagents, into the food or food homogenate, removing the diagnostic reagent holder from the liquid food or food homogenate, and detecting and identifying the presence of a multiplicity of analytes of interest bound to the diagnostic reagent holder, thereby detecting and identifying the presence of a multiplicity of analytes of interest in the food or food homogenate.The invention further provides an enzyme immunoassay for coliform bacteria. This assay comprises inoculating a sample into a suitable medium for coliform reproduction, immobilizing coliforms present in the medium to a suitable surface, treating the surface with an antibody directed to the immobilized coliforms and detecting the presence of the immobilized coliforms immobilized to a suitable surface.

    摘要翻译： 提供了对多组分液体中存在感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与标示有电化学发光化学部分的试剂接触，并能够与感兴趣的分析物结合，将所得样品暴露于电化学能并检测由电化学发光化学部分发射的电磁辐射。 还提供了用于检测和鉴定液体食物或食物匀浆中多种分析物的存在的方法。 这些方法包括将设置有多种试剂的诊断试剂保持器浸入食物或食物匀浆中，从液体食物或食物匀浆中除去诊断试剂载体，以及检测和鉴定多种感兴趣分析物的存在 到诊断试剂保持器，从而检测和鉴定食物或食物匀浆中感兴趣的多种分析物的存在。 本发明还提供了大肠杆菌的酶免疫测定法。 该测定包括将样品接种到用于大肠杆菌繁殖的合适的培养基中，将存在于培养基中的大肠杆菌固定在合适的表面上，用针对固定的大肠杆菌的抗体处理该表面并检测固定在合适的表面上的固定的大肠杆菌的存在。

    Method and device for determining the concentration of heparin in a sample of fluid
    24.
    发明授权
    Method and device for determining the concentration of heparin in a sample of fluid 失效
    用于测定液体样品中肝素浓度的方法和装置

    公开(公告)号：US06731379B2

    公开(公告)日：2004-05-04

    申请号：US10634169

    申请日：2003-08-05

    申请人： Giuseppe Caputo ,  Raffaele Castelli ,  Elena Comoglio ,  Leopoldo Della Ciana ,  Arnaldo Giannetti

    发明人： Giuseppe Caputo ,  Raffaele Castelli ,  Elena Comoglio ,  Leopoldo Della Ciana ,  Arnaldo Giannetti

    IPC分类号： G01N3349

    CPC分类号： G01N33/5308 ,  G01N2400/40

    摘要： A device for determining the concentration of heparin in a fluid sample comprising: a container for holding the fluid sample; a container for holding the dye solution; a mixer for mixing the fluid sample and the dye solution; an illumination source for illuminating a mixture comprising the fluid sample and the dye solution with electromagnetic radiation having a substantially continuous range of wavelengths in the visible range; a detector for detecting the absorption spectrum of the mixture within the substantially continuous range of wavelengths; a recorder for recording the absorption spectrum of the mixture within the substantially continuous range of wavelengths; and a calculator for calculating a spectral parameter.

    摘要翻译： 一种用于确定流体样品中肝素浓度的装置，包括：用于保持流体样品的容器; 用于保持染料溶液的容器; 用于混合流体样品和染料溶液的混合器; 照明源，用于通过电磁辐射照射包含流体样品和染料溶液的混合物，所述电磁辐射具有在可见光范围内的基本上连续的波长范围; 检测器，用于在基本连续的波长范围内检测混合物的吸收光谱; 用于在基本上连续的波长范围内记录混合物的吸收光谱的记录器; 以及用于计算光谱参数的计算器。