Electrochemiluminescent assays
    2.
    发明授权
    Electrochemiluminescent assays 失效
    电化学发光测定

    公开(公告)号:US06916606B2

    公开(公告)日:2005-07-12

    申请号:US10274079

    申请日:2002-10-18

    摘要: Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.Further provided are methods for detecting and identifying the presence of a multiplicity of analytes in a liquid food or food homogenate. These methods comprise immersing a diagnostic reagent holder, provided with a multiplicity of reagents, into the food or food homogenate, removing the diagnostic reagent holder from the liquid food or food homogenate, and detecting and identifying the presence of a multiplicity of analytes of interest bound to the diagnostic reagent holder, thereby detecting and identifying the presence of a multiplicity of analytes of interest in the food or food homogenate.The invention further provides an enzyme immunoassay for coliform bacteria. This assay comprises inoculating a sample into a suitable medium for coliform reproduction, immobilizing coliforms present in the medium to a suitable surface, treating the surface with an antibody directed to the immobilized coliforms and detecting the presence of the immobilized coliforms immobilized to a suitable surface.

    摘要翻译: 提供了对多组分液体中存在感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与标示有电化学发光化学部分的试剂接触,并能够与感兴趣的分析物结合,将所得样品暴露于电化学能并检测由电化学发光化学部分发射的电磁辐射。 还提供了用于检测和鉴定液体食物或食物匀浆中多种分析物的存在的方法。 这些方法包括将设置有多种试剂的诊断试剂保持器浸入食物或食物匀浆中,从液体食物或食物匀浆中除去诊断试剂载体,以及检测和鉴定多种感兴趣分析物的存在 到诊断试剂保持器,从而检测和鉴定食物或食物匀浆中感兴趣的多种分析物的存在。 本发明还提供了大肠杆菌的酶免疫测定法。 该测定包括将样品接种到用于大肠杆菌繁殖的合适的培养基中,将存在于培养基中的大肠杆菌固定在合适的表面上,用针对固定的大肠杆菌的抗体处理该表面并检测固定在合适的表面上的固定的大肠杆菌的存在。

    Transition state analogs
    9.
    发明授权
    Transition state analogs 失效
    过渡状态类比

    公开(公告)号:US5952462A

    公开(公告)日:1999-09-14

    申请号:US333237

    申请日:1994-11-02

    摘要: Antigens capable of eliciting antibodies which can catalyze chemical reactions, in particular, the cleavage or formation of a peptide linkage, comprising a hapten or a hapten and a suitable carrier molecule are disclosed. Haptens include, among others, silicon and boron containing compounds. Antibodies which are catalytically active for chemical reactions, in particular, the cleavage or formation of a selected peptide linkage or an ester bond, and which are elicited by such antigens are disclosed as well as methods for producing the antibodies and methods for catalyzing the cleavage or formation of a peptide linkage or in ester bond in a molecule.

    摘要翻译: 公开了能够引发抗体的抗原,其可以催化化学反应,特别是包含半抗原或半抗原和合适的载体分子的肽键的切割或形成。 除了其他之外,半抗原包括含有硅和硼的化合物。 公开了对于化学反应具有催化活性的抗体,特别是选择的肽键或酯键的切割或形成,并且由这些抗原引发的抗体以及用于产生抗体的方法和用于催化切割的方法 在分子中形成肽键或酯键。