公开(公告)号：US20080241868A1

公开(公告)日：2008-10-02

申请号：US12071333

申请日：2008-02-20

申请人： Leopoldo Della Ciana

发明人： Leopoldo Della Ciana

IPC分类号： C12Q1/28

CPC分类号： C12Q1/28 ,  G01N33/582 ,  Y10S435/975

摘要： Method for increasing the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and a mediator of electrons through the use of a hypernucleophilic acylation catalyst belonging to the class of 4-aminopyridines. It is also described the use in diagnostic assays of chemiluminescent substrates containing said catalysts.

摘要翻译： 通过使用属于4-氨基吡啶类的超亲核酰化催化剂来增加包括鲁米诺，过氧化物酶，氧化剂和电子介体的化学发光反应的光的发射的方法。 还描述了在含有所述催化剂的化学发光底物的诊断测定中的用途。

公开(公告)号：US20050106652A1

公开(公告)日：2005-05-19

申请号：US10274079

申请日：2002-10-18

申请人： Richard Massey ,  Michael Powell ,  Paul Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter Dressick ,  Mohindar Poonian

发明人： Richard Massey ,  Michael Powell ,  Paul Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter Dressick ,  Mohindar Poonian

IPC分类号： C07D473/08 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76 ,  C12Q1/70 ,  C12Q1/26 ,  C12Q1/37 ,  C12Q1/48

CPC分类号： G01N33/76 ,  C07D473/08 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582

摘要： Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety. Further provided are methods for detecting and identifying the presence of a multiplicity of analytes in a liquid food or food homogenate. These methods comprise immersing a diagnostic reagent holder, provided with a multiplicity of reagents, into the food or food homogenate, removing the diagnostic reagent holder from the liquid food or food homogenate, and detecting and identifying the presence of a multiplicity of analytes of interest bound to the diagnostic reagent holder, thereby detecting and identifying the presence of a multiplicity of analytes of interest in the food or food homogenate. The invention further provides an enzyme immunoassay for coliform bacteria. This assay comprises inoculating a sample into a suitable medium for coliform reproduction, immobilizing coliforms present in the medium to a suitable surface, treating the surface with an antibody directed to the immobilized coliforms and detecting the presence of the immobilized coliforms immobilized to a suitable surface.

摘要翻译： 提供了对多组分液体中存在感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与标示有电化学发光化学部分的试剂接触，并能够与感兴趣的分析物结合，将所得样品暴露于电化学能并检测由电化学发光化学部分发射的电磁辐射。 还提供了用于检测和鉴定液体食物或食物匀浆中多种分析物的存在的方法。 这些方法包括将设置有多种试剂的诊断试剂保持器浸入食物或食物匀浆中，从液体食物或食物匀浆中除去诊断试剂载体，以及检测和鉴定多种感兴趣分析物的存在 到诊断试剂保持器，从而检测和鉴定食物或食物匀浆中感兴趣的多种分析物的存在。 本发明还提供了大肠杆菌的酶免疫测定法。 该测定包括将样品接种到用于大肠杆菌繁殖的合适的培养基中，将存在于培养基中的大肠杆菌固定在合适的表面上，用针对固定的大肠菌群的抗体处理该表面并检测固定在合适的表面上的固定的大肠杆菌的存在。

公开(公告)号：US06747159B2

公开(公告)日：2004-06-08

申请号：US10038554

申请日：2002-01-02

申请人： Giuseppe Caputo ,  Leopoldo Della Ciana

发明人： Giuseppe Caputo ,  Leopoldo Della Ciana

IPC分类号： C07D40306

CPC分类号： G01N33/533 ,  C09B23/0008 ,  C09B23/0066 ,  C09B23/02 ,  C09B23/06 ,  C09B23/08

摘要： A symmetric cyanine of the formula: wherein: X is selected from the group consisting of O, S and C(CH3)2; W represents non-metal atoms required to form a benzo-condensed or a naphto-condensed ring; R1 is selected from the group consisting of (CH2)nCH3, (CH2)nSO3− and (CH2)nSO3H, wherein n is an integer selected from 0 to 6 when R1 is (CH2)nCH3, and n is an integer selected from 3 to 6 when R1 is (CH2)nSO3− or (CH2)nSO3H; R2 and R3 are independently selected from the group consisting of H, a sulphonic moiety and a sulphonate moiety and Q is a substituted polymethine bridge.

摘要翻译： 一种下式的对称花青：其中：X选自O，S和C（CH 3）2; W表示形成苯并稠合或萘并稠环所需的非金属原子;选择R1 由（CH 2）n CH 3，（CH 2）n SO 3 - 和（CH 2）n SO 3 H组成的组，其中当R 1是（CH 2）n CH 3时，n是选自0至6的整数，n是选自3至 6，当R 1是（CH 2）n SO 3 - 或（CH 2）n SO 3 H时; R 2和R 3独立地选自H，磺酸基和磺酸酯部分，Q是取代的聚甲炔桥。

公开(公告)号：US5716781A

公开(公告)日：1998-02-10

申请号：US470247

申请日：1995-06-06

申请人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

发明人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

IPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76 ,  C12Q1/00 ,  C12Q1/70 ,  G01N33/48

CPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582 ,  G01N33/76 ,  G01N2458/30 ,  Y10S435/81

摘要： Electrochemiluminiscent moieties having the formula �Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.q (L.sup.5).sub.r (L.sup.6).sub.s !.sub.t (B).sub.u wherein P is a polydentate ligand of Re; L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 and L.sup.6 are ligands of Re, each of which may be the same as or different from each other ligand; B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 or L.sup.6 ; m is an integer equal to or greater than 1; each of n, o, p, q, r and s is zero or an integer; t is an integer equal to or greater than 1; and u is an integer equal to or greater than 1; P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5, L.sup.6 and B being of such composition and number that the chemical moiety can be induced to emit electromagnetic radiation and the total number of bonds to Re provided by the ligands of Re being equal to the coordination of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are disclosed. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

摘要翻译： 具有式[Re（P）m（L1）n（L2）o（L3）p（L4）q（L5）r（L6）s] t（B）u的电化学发光部分其中P是Re的多齿配体; L1，L2，L3，L4，L5和L6是Re的配体，其各自可以彼此相同或不同; B是作为Re的配体或与P，L1，L2，L3，L4，L5或L6中的一个或多个相结合的物质; m是等于或大于1的整数; n，o，p，q，r和s中的每一个为零或整数; t是等于或大于1的整数; u是等于或大于1的整数; P，L1，L2，L3，L4，L5，L6和B具有这样的组成和数量，可以诱导化学部分发射电磁辐射，并且由Re的配体提供的Re的总数相等于 Re的协调被披露。 公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。

公开(公告)号：US09040252B2

公开(公告)日：2015-05-26

申请号：US13167137

申请日：2011-06-23

申请人： Leopoldo Della Ciana ,  Federica Rodeghiero ,  Rossana Perciaccante

发明人： Leopoldo Della Ciana ,  Federica Rodeghiero ,  Rossana Perciaccante

IPC分类号： G01N21/76 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/28

CPC分类号： G01N33/582 ,  C12Q1/28 ,  G01N33/5306

摘要： Method for increasing and regulating the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and an electron mediator (primary enhancer) through the use of an acylation catalyst (secondary enhancer) belonging to the class of N-azoles, i.e., a class of five-membered nitrogen heteroaromatic ring compounds containing at least one other atom of nitrogen. N-azoles, which are especially useful as secondary enhancers are imidazole, 1-methylimidazole, 1,2,3-triazole and 1,2,4-triazole. The invention also describes the use in diagnostic assays of chemiluminescent substrates containing said N-azoles, as secondary enhancers.

摘要翻译： 通过使用属于N-吡咯类的酰化催化剂（二级增强剂）来增加和调节包括鲁米诺，过氧化物酶，氧化剂和电子介体（初级增强剂）的化学发光反应的光的发射的方法， 即一类含有至少一个其他氮原子的五元氮杂芳族环化合物。 作为二次增效剂特别有用的N-吡咯是咪唑，1-甲基咪唑，1,2,3-三唑和1,2,4-三唑。 本发明还描述了在含有所述N-唑类的化学发光底物的诊断测定中用作次级增强剂的用途。

公开(公告)号：US20070269478A1

公开(公告)日：2007-11-22

申请号：US11419611

申请日：2006-05-22

申请人： Corrado Piconi ,  Stefano Rinaldi ,  Leopoldo Della Ciana ,  Antonella Motta

发明人： Corrado Piconi ,  Stefano Rinaldi ,  Leopoldo Della Ciana ,  Antonella Motta

IPC分类号： A61F2/02 ,  B05D5/02

CPC分类号： A61L27/3683 ,  A61L2430/40

摘要： The present invention relates to a new method of preparing acellularized, biocompatible, implantation material starting from a biological material. In particular, the present invention relates to a method of preparing biological material for implantation comprising (a) fixation of the biological material with an aldehyde and (b) detoxification/anticalcification of the fixed biological material, wherein the detoxification/anticalcification step makes use of a novel class of substances of formula: NH2—(CH2)n—X   (I) wherein X is COOH, SO3H, PO3H2 and n is an integer from 2 to 6.

摘要翻译： 本发明涉及从生物材料开始制备非细胞化生物相容性植入材料的新方法。 特别地，本发明涉及一种制备用于植入的生物材料的方法，包括（a）将生物材料与醛固定，和（b）固定生物材料的解毒/抗化学，其中所述解毒/抗化学步骤利用 一类新型的物质：<？in-line-formula description =“In-line formula”end =“lead”？> NH 2 - （CH 2） ）-X（I）<？in-line-formula description =“In-line Formulas”end =“tail”？>其中X是COOH，SO 3， H，PO 3 H 2，n为2〜6的整数。

公开(公告)号：US06630989B1

公开(公告)日：2003-10-07

申请号：US09686219

申请日：2000-10-11

申请人： Giuseppe Caputo ,  Raffaele Castelli ,  Elena Comoglio ,  Leopoldo Della Ciana ,  Arnaldo Giannetti

发明人： Giuseppe Caputo ,  Raffaele Castelli ,  Elena Comoglio ,  Leopoldo Della Ciana ,  Arnaldo Giannetti

IPC分类号： G01N3349

CPC分类号： G01N33/5308 ,  G01N2400/40

摘要： A method for determining the concentration of heparin in a fluid sample comprising: (a) providing a fluid sample containing heparin; (b) adding to the fluid sample a solution of a dye to form a mixture of sample and dye, wherein the dye interacts with the heparin in the sample so that the absorption spectrum of the mixture of sample and dye in the visible range varies as a result of the interaction in a manner quantitatively dependent on the heparin concentration; (c) determining the absorption spectrum in the visible range of the mixture of sample and dye; and (d) calculating a spectral parameter representative of both the reduction in the absorption of the free dye in solution and the increase in the absorption of the dye-heparin complex, the value of the spectral parameter being indicative of the concentration of heparin present in the fluid sample, in order to determine the concentration of heparin present in the fluid sample. The relationship between the value of the spectral parameter and the concentration of the heparin present in the fluid sample has been previously determined by determining the absorption spectra, in the visible range, of a composition comprising the fluid and the dye, in the absence of heparin and in the presence of a plurality of concentrations of heparin, and calculating the relationship between the value of the spectral parameter and the concentration of heparin. A device for performing this method is also provided.

摘要翻译： 一种用于测定流体样品中肝素浓度的方法，包括：（a）提供含有肝素的流体样品; （b）向流体样品中加入染料溶液以形成样品和染料的混合物，其中染料与样品中的肝素相互作用，使得样品和染料在可见范围内的混合物的吸收光谱随着 以定量依赖于肝素浓度的方式相互作用的结果; （c）测定样品和染料混合物的可见范围内的吸收光谱; 和（d）计算代表溶液中游离染料的吸收减少和染料 - 肝素复合物吸收增加的光谱参数，光谱参数值表示存在于肝素中的肝素浓度 流体样品，以确定存在于流体样品中的肝素的浓度。 流体样品中存在的光谱参数的值与肝素的浓度之间的关系已经通过在不存在肝素的情况下通过确定包含流体和染料的组合物在可见范围内的吸收光谱来确定 并在多种浓度的肝素的存在下，计算光谱参数值与肝素浓度之间的关系。 还提供了用于执行该方法的装置。

公开(公告)号：US06316607B1

公开(公告)日：2001-11-13

申请号：US08472425

申请日：1995-06-07

申请人： Richard J. Massey ,  Michael J. Powell ,  Paul A. Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter J. Dressick ,  Mohindar S. Poonian

发明人： Richard J. Massey ,  Michael J. Powell ,  Paul A. Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter J. Dressick ,  Mohindar S. Poonian

IPC分类号： C12Q168

CPC分类号： G01N33/76 ,  C07D473/08 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582

摘要： Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

摘要翻译： 提供了对多组分液体中存在感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与标示有电化学发光化学部分的试剂接触，并能够与感兴趣的分析物结合，将所得样品暴露于电化学能并检测由电化学发光化学部分发射的电磁辐射。

公开(公告)号：US20120009603A1

公开(公告)日：2012-01-12

申请号：US13167137

申请日：2011-06-23

申请人： Leopoldo DELLA CIANA ,  Federica RODEGHIERO ,  Rossana PERCIACCANTE

发明人： Leopoldo DELLA CIANA ,  Federica RODEGHIERO ,  Rossana PERCIACCANTE

IPC分类号： G01N21/76

CPC分类号： G01N33/582 ,  C12Q1/28 ,  G01N33/5306

摘要： Method for increasing and regulating the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and an electron mediator (primary enhancer) through the use of an acylation catalyst (secondary enhancer) belonging to the class of N-azoles., i.e., a class of five-membered nitrogen heteroaromatic ring compounds containing at least one other atom of nitrogen. N-azoles, which are especially useful as secondary enhancers are imidazole, 1-methylimidazole, 1,2,3-triazole and 1,2,4-triazole. The invention also describes the use in diagnostic assays of chemiluminescent substrates containing said N-azoles, as secondary enhancers.

摘要翻译： 通过使用属于N-唑类的酰化催化剂（二级增强剂）来增加和调节包括鲁米诺，过氧化物酶，氧化剂和电子介体（初级增强剂）在内的化学发光反应的光的发射的方法。 ，即一类含有至少一个其他氮原子的五元氮杂芳族环化合物。 作为二次增效剂特别有用的N-吡咯是咪唑，1-甲基咪唑，1,2,3-三唑和1,2,4-三唑。 本发明还描述了在含有所述N-唑类的化学发光底物的诊断测定中用作次级增强剂的用途。

公开(公告)号：US20110092676A1

公开(公告)日：2011-04-21

申请号：US12908145

申请日：2010-10-20

申请人： Leopoldo DELLA CIANA

发明人： Leopoldo DELLA CIANA

IPC分类号： C07K1/13 ,  G01N1/28 ,  C07H1/00

CPC分类号： G01N33/582 ,  C07H1/00 ,  C07K1/13 ,  C12Q1/6806 ,  G01N33/533 ,  Y10T436/25

摘要： The present invention relates to a kit for the labelling of biomolecules bearing reactive amino or hydroxyl groups. The kit consists of a Reagent A and a Reagent B, individually packaged, comprising: a mixture of a carboxylated labelling compound and a tertiary amine (Reagent A); and a coupling reagent (Reagent B). Upon contacting Reagent A with Reagent B, the carboxylated labelling compound is activated in-situ by the coupling reagent (a guanidinium, or uranium salt) in the presence of the tertiary amine. The active form of the carboxylated labelling compound is reacted with a biomolecules bearing reactive amino or hydroxyl groups, with formation of a stable covalent bond between the carboxylated labelling compound and the biomolecule.

摘要翻译： 本发明涉及用于标记具有反应性氨基或羟基的生物分子的试剂盒。 试剂盒由试剂A和试剂B单独包装，包括：羧化标记化合物和叔胺（试剂A）的混合物; 和偶联试剂（试剂B）。 当试剂A与试剂B接触时，羧化标记化合物在叔胺存在下通过偶联试剂（胍或铀盐）原位活化。 羧化标记化合物的活性形式与带有反应性氨基或羟基的生物分子反应，在羧化标记化合物和生物分子之间形成稳定的共价键。