公开(公告)号：US5811236A

公开(公告)日：1998-09-22

申请号：US468524

申请日：1995-06-06

申请人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

发明人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

IPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76 ,  C07H21/04 ,  C07K15/28 ,  G01N23/53

CPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582 ,  G01N33/76 ,  G01N2458/30 ,  Y10S435/81

摘要： Electrochemiluminiscent moieties having the formula �Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.q (L.sup.5).sub.r (L.sup.6).sub.s !.sub.t (B).sub.u wherein P is a polydentate ligand of Re; L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 and L.sup.6 are ligands of Re, each of which may be the same as or different from each other ligand; B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 or L.sup.6 ; m is an integer equal to or greater than 1; each of n, o, p, q, r and s is zero or an integer; t is an integer equal to or greater than 1; and u is an integer equal to or greater than 1; P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5, L.sup.6 and B being of such composition and number that the chemical moiety can be induced to emit electromagnetic radiation and the total number of bonds to Re provided by the ligands of Re being equal to the coordination of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are disclosed. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

摘要翻译： 具有式[Re（P）m（L1）n（L2）o（L3）p（L4）q（L5）r（L6）s] t（B）u的电化学发光部分，其中P是Re的多齿配体; L1，L2，L3，L4，L5和L6是Re的配体，其各自可以彼此相同或不同; B是作为Re的配体或与P，L1，L2，L3，L4，L5或L6中的一个或多个相结合的物质; m是等于或大于1的整数; n，o，p，q，r和s中的每一个为零或整数; t是等于或大于1的整数; u是等于或大于1的整数; P，L1，L2，L3，L4，L5，L6和B具有这样的组成和数量，可以诱导化学部分发射电磁辐射，并且由Re的配体提供的Re的总数相等于 Re的协调被披露。 公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。

公开(公告)号：US5716781A

公开(公告)日：1998-02-10

申请号：US470247

申请日：1995-06-06

申请人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

发明人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

IPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76 ,  C12Q1/00 ,  C12Q1/70 ,  G01N33/48

CPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582 ,  G01N33/76 ,  G01N2458/30 ,  Y10S435/81

摘要： Electrochemiluminiscent moieties having the formula �Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.q (L.sup.5).sub.r (L.sup.6).sub.s !.sub.t (B).sub.u wherein P is a polydentate ligand of Re; L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 and L.sup.6 are ligands of Re, each of which may be the same as or different from each other ligand; B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 or L.sup.6 ; m is an integer equal to or greater than 1; each of n, o, p, q, r and s is zero or an integer; t is an integer equal to or greater than 1; and u is an integer equal to or greater than 1; P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5, L.sup.6 and B being of such composition and number that the chemical moiety can be induced to emit electromagnetic radiation and the total number of bonds to Re provided by the ligands of Re being equal to the coordination of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are disclosed. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

摘要翻译： 具有式[Re（P）m（L1）n（L2）o（L3）p（L4）q（L5）r（L6）s] t（B）u的电化学发光部分其中P是Re的多齿配体; L1，L2，L3，L4，L5和L6是Re的配体，其各自可以彼此相同或不同; B是作为Re的配体或与P，L1，L2，L3，L4，L5或L6中的一个或多个相结合的物质; m是等于或大于1的整数; n，o，p，q，r和s中的每一个为零或整数; t是等于或大于1的整数; u是等于或大于1的整数; P，L1，L2，L3，L4，L5，L6和B具有这样的组成和数量，可以诱导化学部分发射电磁辐射，并且由Re的配体提供的Re的总数相等于 Re的协调被披露。 公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。

公开(公告)号：US06468741B1

公开(公告)日：2002-10-22

申请号：US09157788

申请日：1998-09-21

申请人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

发明人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

IPC分类号： C12Q168

CPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582 ,  G01N33/76 ,  G01N2458/30 ,  Y10S435/81

摘要： A labeled substance comprising a biological substance linked to a luminescent rhenium-containing label. Qualitative and quantitative electrochemiluminescent assays using the same. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

摘要翻译： 包含与发光含铼标记物连接的生物物质的标记物质。 使用相同的定性和定量电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。

公开(公告)号：US5591581A

公开(公告)日：1997-01-07

申请号：US227898

申请日：1994-04-15

申请人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo D. Ciana

发明人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo D. Ciana

IPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76

CPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582 ,  G01N33/76 ,  G01N2458/30 ,  Y10S435/81

摘要： Electrochemiluminescent moieties having the formula(Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.t (B).sub.uwhereinP is a polydentate ligand of Re;L.sup.1, L.sup.2, L.sup.3 and L.sup.4 are ligands of Re, each of which may be the same as or not the same as each other ligand;B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3 and L.sup.4 ;m is an integer equal to or greater than 1;each of n, o, p, q, r and s is zero or an integer;t is an integer equal to or greater than 1; andu is an integer equal to or greater than 1;P, L.sup.1, L.sup.2, L.sup.3, L.sup.4 and B being of such composition and number that the chemical moiety can be induced to electrochemiluminesce and the total number of bonds to Re provided by the ligands of Re being equal to the coordination number of Re are disclosed.Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are also disclosed.

摘要翻译： 具有式（Re（P）m（L1）n（L2）o（L3）p（L4）t（B）u的电化学发光部分，其中P是Re; L1，L2，L3和L4的多齿配体， Re，其各自可以与每个其它配体相同或不相同; B是作为Re的配体或与P，L1，L2，L3和L4中的一个或多个相结合的物质; m是 整数等于或大于1; n，o，p，q，r和s中的每一个为零或整数; t为等于或大于1的整数; u为等于或大于1的整数; P，L1，L2，L3，L4和B具有化学部分可以被诱导为电化学发光的组成和数量，并且Re的配体提供的Re的总数等于Re的配位数 还公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量电化学发光测定。

公开(公告)号：US5746974A

公开(公告)日：1998-05-05

申请号：US467028

申请日：1995-06-06

申请人： Richard J. Massey ,  Gary F. Blackburn ,  Elizabeth W. Wilkins ,  Jonathan K. Leland

发明人： Richard J. Massey ,  Gary F. Blackburn ,  Elizabeth W. Wilkins ,  Jonathan K. Leland

IPC分类号： C12Q1/68 ,  G01N1/40 ,  G01N21/66 ,  G01N21/69 ,  G01N21/76 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N21/69 ,  C12Q1/6825 ,  G01N21/76 ,  G01N33/54313 ,  G01N33/54326 ,  G01N33/54366 ,  G01N33/5438 ,  G01N33/582 ,  G01N1/40 ,  G01N2458/30

摘要： What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing said sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of coated magnetic particles capable of specifically binding with the analyte and/or said assay-performance-substance; incubating said composition to form a complex which includes a particle and said labeled component; magnetically collecting said complex at the surface of an electrode; inducing said label to luminesce by contacting it with a trigger, said trigger being formed in-situ by conversion of a precursor molecule upon introduction of electrochemical energy; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.

摘要翻译： 描述了用于对样品中存在的感兴趣分析物进行结合测定的方法和装置。 所述方法包括以下步骤：形成含有所述样品的组合物，包含与触发时能够化学发光的标记化合物连接的组分的测定性能物质，以及能够与分析物特异性结合的多个包被的磁性颗粒， /或所述测定性能物质; 孵育所述组合物以形成包含颗粒和所述标记组分的复合物; 在电极表面磁性收集所述复合物; 通过使其与触发器接触来诱导所述标签发光，所述触发器通过在引入电化学能量时转化前体分子而原地形成; 并测量发射的发光以测量样品中感兴趣的分析物的存在。

公开(公告)号：US06362011B1

公开(公告)日：2002-03-26

申请号：US09243215

申请日：1999-02-02

申请人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

发明人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

IPC分类号： G01N33551

CPC分类号： C01B31/0213 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/152 ,  C01B32/174 ,  C01B2202/34 ,  C01B2202/36 ,  C07K17/06 ,  D01F9/12 ,  D01F11/14 ,  G01N21/69 ,  G01N21/76 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/582 ,  G01N2030/8863 ,  G11C13/025 ,  H01M4/60 ,  Y10S435/968 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S977/737 ,  Y10S977/742

摘要： Graphitic nanotubes, which include tubular fullerenes (commonly called “buckytubes”) and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

摘要翻译： 石墨纳米管，其包括通过化学取代官能化的管状富勒烯（通常称为“巴氏管”）和原纤维，被用作电致化学发光测定中的固体支持物。 石墨纳米管在用于测定之前用官能团生物分子进行化学修饰。 电化学发光钌络合物与官能团生物分子修饰的纳米管的关联允许通过多种形式检测包括核酸，抗原，酶和酶底物的分子。

公开(公告)号：US5866434A

公开(公告)日：1999-02-02

申请号：US611347

申请日：1996-03-06

申请人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

发明人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

IPC分类号： B01J20/281 ,  A61K47/04 ,  B01J20/20 ,  B01J21/18 ,  B01J32/00 ,  C01B31/02 ,  C07K16/44 ,  C07K17/06 ,  D01F9/12 ,  D01F11/14 ,  G01N21/69 ,  G01N21/76 ,  G01N21/78 ,  G01N30/88 ,  G01N33/543 ,  G01N33/553 ,  G01N33/573 ,  G01N33/58 ,  H01M4/60 ,  G01N33/551 ,  C12Q1/32 ,  C12Q1/37

CPC分类号： C01B31/0213 ,  B82Y30/00 ,  B82Y40/00 ,  C01B31/0273 ,  C07K17/06 ,  D01F11/14 ,  D01F9/12 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/582 ,  H01M4/60 ,  C01B2202/34 ,  C01B2202/36 ,  G01N2030/8863 ,  G01N21/69 ,  G01N21/76 ,  G11C13/025 ,  Y10S435/968 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S977/737 ,  Y10S977/742

摘要： Graphitic nanotubes, which include tubular fullerenes (commonly called "buckytubes") and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

公开(公告)号：US5770459A

公开(公告)日：1998-06-23

申请号：US348749

申请日：1994-12-01

申请人： Richard J. Massey ,  Gary F. Blackburn ,  Elizabeth W. Wilkins ,  Jonathan K. Leland

发明人： Richard J. Massey ,  Gary F. Blackburn ,  Elizabeth W. Wilkins ,  Jonathan K. Leland

IPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N1/40 ,  G01N21/66 ,  G01N21/69 ,  G01N21/76 ,  G01N33/533 ,  G01N33/543 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76 ,  G01N33/53

CPC分类号： G01N21/69 ,  C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/6825 ,  C12Q1/686 ,  G01N21/76 ,  G01N33/533 ,  G01N33/54313 ,  G01N33/54326 ,  G01N33/54366 ,  G01N33/5438 ,  G01N33/569 ,  G01N33/582 ,  G01N33/585 ,  G01N33/76 ,  G01N1/40 ,  G01N2458/30

摘要： What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing said sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of coated magnetic particles capable of specifically binding with the analyte and/or said assay-performance-substance; incubating said composition to form a complex which includes a particle and said labeled component; magnetically collecting said complex at the surface of an electrode; inducing said label to luminesce by contacting it with a trigger, said trigger being formed in-situ by conversion of a precursor molecule upon introduction of electrochemical energy; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.

摘要翻译： 描述了用于对样品中存在的感兴趣分析物进行结合测定的方法和装置。 所述方法包括以下步骤：形成含有所述样品的组合物，包含与触发时能够化学发光的标记化合物连接的组分的测定性能物质，以及能够与分析物特异性结合的多个包被的磁性颗粒， /或所述测定性能物质; 孵育所述组合物以形成包含颗粒和所述标记组分的复合物; 在电极表面磁性收集所述复合物; 通过使其与触发器接触来诱导所述标签发光，所述触发器通过在引入电化学能量时转化前体分子而原地形成; 并测量发射的发光以测量样品中感兴趣的分析物的存在。

公开(公告)号：US07052861B2

公开(公告)日：2006-05-30

申请号：US10007526

申请日：2001-12-05

申请人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

发明人： Richard J. Massey ,  Mark T. Martin ,  Liwen Dong ,  Ming Lu ,  Alan Fischer ,  Fabian Jameison ,  Pam Liang ,  Robert Hoch ,  Jonathan K. Leland

IPC分类号： G01N33/573 ,  G01N33/533 ,  G01N33/553 ,  G01N21/76 ,  C12Q1/32

CPC分类号： C01B31/0213 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/152 ,  C01B32/174 ,  C01B2202/34 ,  C01B2202/36 ,  C07K17/06 ,  D01F9/12 ,  D01F11/14 ,  G01N21/69 ,  G01N21/76 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/582 ,  G01N2030/8863 ,  G11C13/025 ,  H01M4/60 ,  Y10S435/968 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S977/737 ,  Y10S977/742

摘要： Graphitic nanotubes, which include tubular fullerenes (commonly called “buckytubes”) and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

公开(公告)号：US5935779A

公开(公告)日：1999-08-10

申请号：US346832

申请日：1994-11-30

申请人： Richard J. Massey ,  Jonathan K. Leland ,  Haresh P. Shah ,  John H. Kenten ,  Jack E. Goodman ,  George E. Lowke ,  Yuzaburo Namba ,  Gary F. Blackburn

发明人： Richard J. Massey ,  Jonathan K. Leland ,  Haresh P. Shah ,  John H. Kenten ,  Jack E. Goodman ,  George E. Lowke ,  Yuzaburo Namba ,  Gary F. Blackburn

IPC分类号： C07H21/00 ,  C12Q1/68 ,  G01N1/40 ,  G01N21/66 ,  G01N21/69 ,  G01N21/76 ,  G01N33/543 ,  G01N33/58

CPC分类号： C07H21/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/686 ,  G01N21/66 ,  G01N21/69 ,  G01N21/76 ,  G01N33/54313 ,  G01N33/54326 ,  G01N33/54366 ,  G01N33/5438 ,  G01N33/582 ,  G01N1/40 ,  G01N2458/30

摘要： The invention relates to methods of performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence at an electrode of interest. Particles are employed in the method, which are then collected in a zone at which electrochemiluminescence can be induced, wherein the amount of induced electrochemilurninescence is related to the amount of analyte in the sample.

摘要翻译： 本发明涉及基于感兴趣的电极处的电化学发光对样品中存在的感兴趣分析物进行结合测定的方法。 在该方法中使用颗粒，然后将其收集在可以诱导电化学发光的区域中，其中诱导的电化学发光的量与样品中分析物的量相关。