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公开(公告)号：US20170015988A1
公开(公告)日：2017-01-19
申请号：US15208849
申请日：2016-07-13
发明人： Alexandra Merkx-Jacques , David Woodhall , Mark Scaife , Roberto E. Armenta , Denise Muise , Holly Rasmussen , Jeremy Benjamin
CPC分类号： C12N9/92 , C07K14/40 , C07K2319/21 , C12N9/1205 , C12Y207/01017 , C12Y503/01005
摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.
摘要翻译： 本文提供了具有两个或多个拷贝的编码木糖异构酶的核酸序列的重组微生物，其中编码木糖异构酶的核酸是外源核酸。 任选地，重组微生物包括编码木酮糖激酶和/或编码木糖转运蛋白的至少一个核酸序列的至少一个核酸序列。 所提供的重组微生物能够在作为碳源的木糖上生长。
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公开(公告)号：US20160281054A1
公开(公告)日：2016-09-29
申请号：US15079735
申请日：2016-03-24
发明人： Laura Purdue , Michael Milway , Kevin Berryman , Mercia Valentine , Zhiyong Sun , Roberto E. Armenta
CPC分类号： C12N1/32 , C12N1/12 , C12P7/6427 , C12P7/6472
摘要： Provided is a method of culturing one or more microorganisms. The method includes culturing one or more microorganisms in a medium comprising crude glycerol at a first concentration level, feeding to the media an additional amount of crude glycerol, once the first concentration of glycerol is reduced to a first threshold level, at a concentration sufficient to achieve the first concentration level, monitoring the crude glycerol concentration until the first concentration level of the crude glycerol is reduced to the first threshold level. The steps may be repeated until a desired microorganism cell density is achieved.
摘要翻译： 提供培养一种或多种微生物的方法。 该方法包括在包含粗甘油的培养基中培养一种或多种微生物，所述培养基以第一浓度水平，一旦甘油的第一浓度降低至第一阈值水平，向培养基中加入额外量的粗甘油，浓度足以 达到第一浓度水平，监测粗甘油浓度，直到粗甘油的第一浓度水平降至第一阈值水平。 可以重复这些步骤，直到达到所需的微生物细胞密度。
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公开(公告)号：US20160108453A1
公开(公告)日：2016-04-21
申请号：US14882742
申请日：2015-10-14
CPC分类号： C12Q1/06 , C12N1/04 , C12N1/10 , C12P7/6409 , C12P7/6427
摘要： Provided herein are methods of culturing a microorganism. The method includes providing a container comprising one or more microorganisms and medium, wherein the microorganisms and medium form a start volume. The microorganisms and medium are cultured until the culture reaches a threshold indicator, wherein culturing comprises feeding one or more carbon sources to the culture and wherein the culture is at a threshold volume when the threshold indicator is reached. The method also includes harvesting a portion of the threshold volume to leave a residual volume that is 40% or less of the start volume and adding fresh medium to the container in an amount to return the volume of the culture to the start volume.
摘要翻译： 本文提供培养微生物的方法。 该方法包括提供包含一种或多种微生物和培养基的容器，其中微生物和培养基形成起始体积。 培养微生物和培养基直到培养物达到阈值指示剂，其中培养包括向培养物中加入一种或多种碳源，并且其中当达到阈值指示剂时，培养物处于阈值体积。 该方法还包括收获阈值体积的一部分以留下起始体积的40％或更小的残余体积，并将新鲜培养基以将培养物体积返回到起始体积的量添加到容器中。
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公开(公告)号：US20240182934A1
公开(公告)日：2024-06-06
申请号：US18525184
申请日：2023-11-30
IPC分类号： C12P7/6431 , C12N1/20 , C12N9/02 , C12N9/10 , C12N15/52 , C12P7/6432
CPC分类号： C12P7/6431 , C12N1/205 , C12N9/0071 , C12N9/1029 , C12N15/52 , C12P7/6432 , C12Y114/19001 , C12Y114/19006
摘要： Provided herein are engineered microorganisms comprising one or more genes involved in fatty acid metabolism. For example, provided is an engineered microorganism comprising a first nucleic acid sequence encoding an elongase and a second nucleic acid sequence encoding a desaturase, wherein the first and second nucleic acid sequences are operably linked to promoter. Also provided are methods of making and using the engineered microorganisms. Also provided are microbial oils comprising fatty acids, wherein the fatty acids comprise C20:3(n-6) (di-homo-γ-linoleic acid) and C20:5(n-3) eicosapentaenoic acid (EPA). In addition, provided herein are methods of promoting conversion of saturated fatty acids to unsaturated fatty acids by transforming microorganisms with nucleic acid encoding polypeptides involved in the fatty acid synthesis pathway to generated increased conversion of saturated fatty acids to unsaturated fatty acids.
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公开(公告)号：US11959120B2
公开(公告)日：2024-04-16
申请号：US17083045
申请日：2020-10-28
发明人： Zhiyong Sun , Alan Sothern , Kevin Berryman , Mercia Valentine , Michael Milway , Laura Purdue , Roberto E. Armenta
IPC分类号： C12P7/6427 , C12P7/6463
CPC分类号： C12P7/6427 , C12P7/6463
摘要： Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.
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公开(公告)号：US11746363B2
公开(公告)日：2023-09-05
申请号：US16989718
申请日：2020-08-10
IPC分类号： C11B1/10 , C12P7/6463 , C12P7/6472 , C12P7/6427
CPC分类号： C11B1/10 , C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.
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27.发明申请公开(公告)号：US20220177934A1
公开(公告)日：2022-06-09
申请号：US17533771
申请日：2021-11-23
IPC分类号： C12P7/6427 , A23K20/158 , C12N1/14 , C12P7/6472 , A23L33/115 , C12N1/10 , C12N1/12 , A23K20/147 , A23K50/00 , A23K10/18 , A23L33/135 , C12P7/6409 , C12P21/00 , C12P23/00
摘要： Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.
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公开(公告)号：US11345943B2
公开(公告)日：2022-05-31
申请号：US16864128
申请日：2020-04-30
发明人： Zhiyong Sun , Michael Milway , Kevin Berryman , Mercia Valentine , Roberto E. Armenta , Laura Purdue
IPC分类号： C12P7/64 , C12Q1/06 , C12P7/6463 , C12P7/6472 , C12N1/12 , C12N1/14 , C12N1/04 , C12P7/6409 , C12P7/6427
摘要： Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.
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公开(公告)号：US20210310032A1
公开(公告)日：2021-10-07
申请号：US17221810
申请日：2021-04-04
发明人： Joshua Lowrey , Zhiyong Sun , Roberto E. Armenta , Denise Muise
IPC分类号： C12P7/64
摘要： Provided herein are microbial oils and methods of making and using microbial oils with high levels of omega-3 fatty acids. Specifically, provided is a microbial oil comprising at least 85% total fatty acids, wherein the total fatty acids comprise at least 50% DHA. Also provided is a method of making a biomass comprising culturing an oil-producing microorganism in a culture medium comprising a fatty acid synthesis inhibitor, wherein the biomass comprises at least 500 mg/g oil.
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公开(公告)号：US10907115B2
公开(公告)日：2021-02-02
申请号：US16050857
申请日：2018-07-31
发明人： Xuan Jiang , Dorothy Dennis , Roberto E. Armenta
摘要： Provided herein are methods for winterizing oil. The methods include heating the oil to a first temperature and maintaining the oil at the first temperature for a first period of time; reducing the first temperature of the oil after the first period of time to a second temperature over a second period of time, wherein reducing the first temperature produces a first solid fraction and first liquid fraction of the oil; removing the first solid fraction from the oil; reducing the second temperature of the first liquid fraction of the oil over a third period of time to a third temperature, wherein reducing the second temperature of the oil produces a second solid fraction and second liquid fraction of the oil; removing the second solid fraction from the oil; and recovering the second liquid fraction of the oil.
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