公开(公告)号：US07413882B2

公开(公告)日：2008-08-19

申请号：US11078921

申请日：2005-03-10

Applicant: Randy Berka ,  Joel Cherry

Inventor： Randy Berka ,  Joel Cherry

IPC: C12P19/02 ,  C12P7/06 ,  C12P21/06 ,  C12N9/24 ,  C12N7/00 ,  C12N1/12 ,  D21C3/00

CPC classification number: C12P19/14 ,  C12P5/023 ,  C12P7/00 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/26 ,  C12P7/28 ,  C12P7/40 ,  C12P13/04 ,  C12P17/04 ,  C12P19/02 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.

Abstract translation: 本发明涉及将植物细胞壁多糖转化为一种或多种产品的方法，其包括：用有效量的重组微生物的消耗的全发酵液处理植物细胞壁多糖，其中重组微生物表达一种或多种异源 编码将植物细胞壁多糖降解或转化成一种或多种产物的酶的基因。 本发明还涉及有机物质的制造方法，其特征在于，包括：（a）用有效量的重组微生物用的全部发酵肉汤糖化植物细胞壁多糖，其中所述重组微生物表达一种或多种编码酶的异源基因 其将植物细胞壁多糖降解或转化为糖化物质; （b）用一种或多种发酵微生物发酵步骤（a）的糖化物质; 和（c）从发酵中回收有机物质。

公开(公告)号：US20050069934A1

公开(公告)日：2005-03-31

申请号：US10950009

申请日：2004-09-24

Applicant: Randy Berka ,  Elena Bashkirova ,  Michael Rey

Inventor： Randy Berka ,  Elena Bashkirova ,  Michael Rey

IPC: C12M1/34 ,  C12Q1/68

CPC classification number: C12Q1/6895 ,  C12Q2600/158

Abstract: The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

Abstract translation: 本发明涉及使用含有里氏木霉EST或SSH克隆的微阵列来监测第一丝状真菌细胞中多个基因的差异表达相对于一个或多个第二丝状真菌细胞中相同基因表达的方法，或其组合 。 本发明还涉及包含用于监测丝状真菌细胞中多个基因的表达的这种阵列特征的计算机可读介质和底物。

公开(公告)号：US20150218567A1

公开(公告)日：2015-08-06

申请号：US14426557

申请日：2013-09-26

Applicant: Randy BERKA ,  Barbara CHERRY ,  NOVOZYMES, INC.

Inventor： Randy Berka ,  Barbara Cherry

IPC: C12N15/75 ,  C12P5/00 ,  C12P5/02 ,  C12P13/04 ,  C12P21/00 ,  C12P7/04

CPC classification number: C12N15/75 ,  C07K14/32 ,  C12P5/007 ,  C12P5/02 ,  C12P5/026 ,  C12P7/04 ,  C12P13/04 ,  C12P21/00 ,  C12P21/02 ,  Y02E50/343

Abstract: Provided herein are Bacillus mutants having improved transformation efficiency, comprising a disruption to an endogenous mecA gene and disruption to an endogenous sinI gene. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.

Abstract translation: 本文提供了具有改善的转化效率的芽孢杆菌突变体，包括内源性mecA基因的破坏和内源性sinI基因的破坏。 还描述了用于产生突变体的方法，使用突变体产生转化体的方法，以及使用突变体产生多肽或发酵产物的方法。

公开(公告)号：US07387875B2

公开(公告)日：2008-06-17

申请号：US10950009

申请日：2004-09-24

Applicant: Randy Berka ,  Elena Bachkirova ,  Michael Rey

Inventor： Randy Berka ,  Elena Bachkirova ,  Michael Rey

IPC: C12Q1/68 ,  C12N1/00 ,  C07H21/04

CPC classification number: C12Q1/6895 ,  C12Q2600/158

Abstract: The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

Abstract translation: 本发明涉及使用含有里氏木霉EST或SSH克隆的微阵列来监测第一丝状真菌细胞中多个基因的差异表达相对于一个或多个第二丝状真菌细胞中相同基因表达的方法，或其组合 。 本发明还涉及包含用于监测丝状真菌细胞中多个基因的表达的这种阵列特征的计算机可读介质和底物。

公开(公告)号：US20050233423A1

公开(公告)日：2005-10-20

申请号：US11078921

申请日：2005-03-10

Applicant: Randy Berka ,  Joel Cherry

Inventor： Randy Berka ,  Joel Cherry

IPC: C07G99/00 ,  C12P5/00 ,  C12P5/02 ,  C12P7/00 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/26 ,  C12P7/28 ,  C12P7/40 ,  C12P13/04 ,  C12P17/04 ,  C12P19/02 ,  C12P19/04 ,  C13B35/00

CPC classification number: C12P19/14 ,  C12P5/023 ,  C12P7/00 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/26 ,  C12P7/28 ,  C12P7/40 ,  C12P13/04 ,  C12P17/04 ,  C12P19/02 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.

Abstract translation: 本发明涉及用于将植物细胞壁多糖转化成一种或多种产品的方法，其包括：用有效量的重组微生物的消耗的全发酵液处理植物细胞壁多糖，其中所述重组微生物表达一种或多种异源 编码将植物细胞壁多糖降解或转化成一种或多种产物的酶的基因。 本发明还涉及有机物质的制造方法，其特征在于，包括：（a）用有效量的重组微生物用的全部发酵肉汤糖化植物细胞壁多糖，其中所述重组微生物表达一种或多种编码酶的异源基因 其将植物细胞壁多糖降解或转化为糖化物质; （b）用一种或多种发酵微生物发酵步骤（a）的糖化物质; 和（c）从发酵中回收有机物质。